Common-use Data System for Food
     Animal Genetics in Taiwan



                                  Ming-Che Wu
          ...
A reservoir of genetic adaptability acts a buffer
against potentially harmful environmental and
economic change.




     ...
System for Food Animal Genetics
               in Taiwan
                                  Phase I (1987~1992):
    Conser...
Common-use
• DNA in food chain
• Biogenetic banks in
  supporting scientific
  research
• Genomic selection on
  farm leve...
乳 Milk




DNA in food chain
                    Meat 肉



           Egg 蛋
Origin
assurance
- market to farm
Web site: www.angrin.tlri.gov.tw
BioUtilization

    Conservation           Biodiversity
                 Species
                                         ...
(source:FAO, 2007)

                   Global Movement of AnGR
     (milk)
 (pork)
(pork)
    (pork)
   (milk)
   (milk)

...
Biogenetic resources are parts of our life.
          Genetic diversity is threatened by
          - genetic erosion
     ...
Genetic
  Banking
   Living form
    Cell/tissue
DNA/RNA/mRNA/
  cDNA/protein
Development of miniature pig for biomedical research in Taiwan
                                                      Ming-...
Development of miniature pig for biomedical research in Taiwan
                                                           ...
Mitasi minipig was developed from sib-mating on hybrid of Duroc
and Lanyu breed.

                                        ...
Fullsib Mating on
coat color selection




                 19
Development of miniature pig for biomedical research in Taiwan
                                                         Mi...
Semen collection and storage
Ex situ conservation of minipig
• Cell Bank
   - Sperm bank, Oocyte bank, Embryo bank
• DNA Bank

    Taiwan Animal Germplasm Center
        http://www.an...
Taiwan Animal Germplasm Center
  http://www.angrin.tlri.gov.tw/
CryoArk for Taiwan




 Cryotanks: 24K*5 + 38K*17 + 80K*2 = 926K
(CryoArk for Taiwan: 46,000 sp. in total)
Oocyte
          Sperm


Embryo



Cell Bank
•K Series - Liquid nitrogen storage with controllable
                      temperatures between (-100°C to -196°C)
  Smal...
Bio-security
          -185℃ Vapor
          storage
                •designed for large
                vial capacities u...
Gene Banking
DNA Banking
2007                                 Living form/Cell/Tissue
                                  DN...
Landrace          Lanyu            Duroc             Taoyuan

            Lee-Sung Inbreeding Mitsae           TLRI Black
...
Impact of common-use breeding data on production efficiency
    2.20

                    Duroc          Landrace       Yo...
Growth performance test for breeding pig
Auction
Elite animal labeled with the breeder name
prior to auction
Performance data of individual pig with its own pedigree along with genotype
information




         Auction price of eac...
On-line Statistics for Auction Data of Elite Pig




Auction                                                 Price (NT$)
 ...
Cow Milk Production in Dairy Herd Improvement System
Regional Statistics on Dairy Farming Sector




                          Cow Milking Performance
         No. of Farmers
...
Profiles of daily milk yield per cow in average and number of DHI farms
(1990 ~ 2008)
                                    ...
Regulation for genomic breeding - Animal Industry Act Article 17
The competent authority may dispatch inspector to examine...
DNA barcoding for pigs
                                                          DNA
Hal-1843 gene for stress (AB), ESR ge...
Native Chicken Barcode
 MMFFAARR
   2-3-5-Z                                       (Red feather)
 PRL-ESR-HSP70-PRLR
  NNEE...
Common-use
• DNA in food chain
• Biogenetic banks in
  supporting scientific
  research
• Genomic selection on
  farm leve...
Acknowledgements        •   Wu Lab
                             –   Mr. Yun-Yu Lai
                             –   Dr. Re...
Common Use Data System For Food Animal Genetics In Taiwan
Common Use Data System For Food Animal Genetics In Taiwan
Common Use Data System For Food Animal Genetics In Taiwan
Common Use Data System For Food Animal Genetics In Taiwan
Common Use Data System For Food Animal Genetics In Taiwan
Common Use Data System For Food Animal Genetics In Taiwan
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Common Use Data System For Food Animal Genetics In Taiwan

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Common Use Data System For Food Animal Genetics In Taiwan

  1. 1. Common-use Data System for Food Animal Genetics in Taiwan Ming-Che Wu Taiwan Livestock Research Institute
  2. 2. A reservoir of genetic adaptability acts a buffer against potentially harmful environmental and economic change. Food security and mileage (Sustainability)
  3. 3. System for Food Animal Genetics in Taiwan Phase I (1987~1992): Conservation farms ● Conservation farms Phase II(1993~1998): ● Web site www.angrin.tlri.gov.tw Web site Phase III(1999~2004): Cryopreservation Bank ● Conservation center Phase IV(2005~2008): Molecular techniques ● Research and Utilization Genetic resources Phase V(2009~2012): ● exchange Global Bioidentification System
  4. 4. Common-use • DNA in food chain • Biogenetic banks in supporting scientific research • Genomic selection on farm level • Web-based barcode system with food mileage
  5. 5. 乳 Milk DNA in food chain Meat 肉 Egg 蛋
  6. 6. Origin assurance - market to farm
  7. 7. Web site: www.angrin.tlri.gov.tw
  8. 8. BioUtilization Conservation Biodiversity Species Species verification Value add Selection Demand New breed/line Tracking Export Genetic improvement Genomic breeding
  9. 9. (source:FAO, 2007) Global Movement of AnGR (milk) (pork) (pork) (pork) (milk) (milk) (chicken meat) (chicken egg) (fur sheep)
  10. 10. Biogenetic resources are parts of our life. Genetic diversity is threatened by - genetic erosion - habitat loss - social value sinking - economic setback - food print mileage From Farm to DNA Bank
  11. 11. Genetic Banking Living form Cell/tissue DNA/RNA/mRNA/ cDNA/protein
  12. 12. Development of miniature pig for biomedical research in Taiwan Ming-Che Wu, PhD Taiwan Livestock Research Institute www.angrin.tlri.gov.tw mcwu@mail.tlri.gov.tw Taiwan Lanyu
  13. 13. Development of miniature pig for biomedical research in Taiwan Ming-Che Wu, PhD Taiwan Livestock Research Institute www.angrin.tlri.gov.tw mcwu@mail.tlri.gov.tw Lanyu 200 Hybrid Hybrid Inbreeding Duroc GPI-CRC-PGD Genotyped Mitsae (Lanyu 50) Spotty Lanyu Line (Lanyu 300) (Lanyu 100) (50% Lanyu + 50% Duroc)
  14. 14. Mitasi minipig was developed from sib-mating on hybrid of Duroc and Lanyu breed. 1989 1990 1991 1992 1993 1994 1995 (Lanyu 50) 50
  15. 15. Fullsib Mating on coat color selection 19
  16. 16. Development of miniature pig for biomedical research in Taiwan Ming-Che Wu, PhD Taiwan Livestock Research Institute Lanyu 300 www.angrin.tlri.gov.tw mcwu@mail.tlri.gov.tw Lanyu 50 Lanyu 100 Lanyu 200 Conservation to Bio- utilization (1987-2008) Lanyu 300 Lanyu 100 Lanyu Lanyu 100 Lanyu 400 Lanyu 50 Black 1980 Lanyu White Spotty Lanyu 2005 1993
  17. 17. Semen collection and storage Ex situ conservation of minipig
  18. 18. • Cell Bank - Sperm bank, Oocyte bank, Embryo bank • DNA Bank Taiwan Animal Germplasm Center http://www.angrin.tlri.gov.tw/
  19. 19. Taiwan Animal Germplasm Center http://www.angrin.tlri.gov.tw/
  20. 20. CryoArk for Taiwan Cryotanks: 24K*5 + 38K*17 + 80K*2 = 926K (CryoArk for Taiwan: 46,000 sp. in total)
  21. 21. Oocyte Sperm Embryo Cell Bank
  22. 22. •K Series - Liquid nitrogen storage with controllable temperatures between (-100°C to -196°C) Small LN Tank -185℃ Vapor storage •designed for large vial capacities up to 6000 vials (2 ml vial) in box type racks. -80℃ Freezer
  23. 23. Bio-security -185℃ Vapor storage •designed for large vial capacities up to 6000 vials (2 ml vial) in box type racks.
  24. 24. Gene Banking DNA Banking 2007 Living form/Cell/Tissue DNA/RNA/mRNA/cDNA/Protein Cattle 6180 Water Buffalo 58 Goat 3127 Oocyte Sheep 14 Horse 14 Rabbit 88 Swine 25129 Chicken 11650 Muscovy 151 Embryo Duck 689 Hybrid Duck 128 Goose 431 Turkey 14 Black Swan 135 Quail 50 Total 47858
  25. 25. Landrace Lanyu Duroc Taoyuan Lee-Sung Inbreeding Mitsae TLRI Black Spotty Meishing Yorkshire Hampshire Berkshire LY Pig Breeding LYD Hog
  26. 26. Impact of common-use breeding data on production efficiency 2.20 Duroc Landrace Yorkshire 2.15 Feed efficiency(Feed/Gain) from 40 to 110 kg of body weight 2.10 2.05 2.00 2000 2001 2002 2003 2004 2005 2006 2007 Birth year of boar
  27. 27. Growth performance test for breeding pig
  28. 28. Auction
  29. 29. Elite animal labeled with the breeder name prior to auction
  30. 30. Performance data of individual pig with its own pedigree along with genotype information Auction price of each pig and buyer soon after sold
  31. 31. On-line Statistics for Auction Data of Elite Pig Auction Price (NT$) No. of Breeder and Buyer Year
  32. 32. Cow Milk Production in Dairy Herd Improvement System
  33. 33. Regional Statistics on Dairy Farming Sector Cow Milking Performance No. of Farmers County
  34. 34. Profiles of daily milk yield per cow in average and number of DHI farms (1990 ~ 2008) 400 25 Daily Milk Yield (kg) 24 DHI Farm (N) 350 23 22 300 21 250 20 19 4.6 4 3.8 4.4 200 3.6 4.2 3.4 18 3.2 4 3 3.8 2.8 Profile on milk quality in Protein % Protein % (milk yield adjusted) 2.6 3.6 2.4 17 protein percentage from 2.2 3.4 150 those farms having more 2 3.2 1.8 than 22 kg of milk yield 1.6 3 16 1.4 recorded in 2007. 2.8 1.2 1 2.6 .1 .2 .07 .14 .32 .37 .39 .59 .77 .86 .15 .39 .45 .52 .55 .76 .81 .04 .11 .24 .36 .62 .69 .79 .92 .07 .17 .24 .52 .97 .28 .51 .57 .28 .42 .71 .74 .05 .16 .51 23 29 22 22 22 22 22 22 22 22 23 23 23 23 23 23 23 24 24 24 24 24 24 24 24 25 25 25 25 25 26 26 26 27 27 27 27 28 30 34 15 100 07 11 03 07 11 03 07 11 03 07 11 03 07 11 03 07 11 03 07 11 03 07 11 03 07 11 03 99 99 00 00 00 01 01 01 02 02 02 03 03 03 04 04 04 05 05 05 06 06 06 07 07 07 08 19 19 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
  35. 35. Regulation for genomic breeding - Animal Industry Act Article 17 The competent authority may dispatch inspector to examine or test the breeding flock, breeding stock, facilities, pedigree registry and related records of breeders, and the breeders shall not evade, interfere with or object to such examination or testing. Breeding flock or breeding stock found to have contracted notified disease or have hereditary disease during the aforesaid examination or testing shall be banned from breeding. The aforesaid inspectors shall present their identification when carrying out their duty. Pig Cow Goat Chicken Duck Goose Deer Rabbit Hal-1843 BLAD G6S HSP70 PRL PRL IGF2 GPI ESR DUMP IGF2 PRLR ESR ESR XY PGD Gene HFABP-H CITR mtDN PRL ZW ZW D5FAD XY A HFABP-ad CVM ESR mtDNA mtDNA mtDNA IGF2 IGF2-In3 XY mtDNA mtDNA IGF2-In7 GH ZW D5FAD mtDNA D-Loop SW18 SW1943 SW2588 PRLR
  36. 36. DNA barcoding for pigs DNA Hal-1843 gene for stress (AB), ESR gene for prolificacy (MN) and 16,613bp mtDNA for maternal origin, especially on D-loop region (1,175bp) of 29 SNPs and UTRn (tacacgtgcg 10bp)17~27 in various breeds of pigs. AB+MN+18SNP+UTR+11SNP mtDNA D-Loop AAMNcaattgctcgtttctcag24tgctcgaaacc A0021-02 Lanyu T0596-01 Taoyuan AAMMcaattgcttgttttccag24tgctcgaaact L0074-10 Landrace AAMNttgccattcaccctccaa27tgcttaaaatt Y0150-03 Yorkshire AAMNcaattgcctgttttccag25tccccgaaacc AANNttgccattcaccctccaa26tgcttaaaatc D0167-05 Duroc
  37. 37. Native Chicken Barcode MMFFAARR 2-3-5-Z (Red feather) PRL-ESR-HSP70-PRLR NNEEBBPP Chicken genome (Black feather) (Silky feather/Black meat) 2-3-5-Z-mtDNA PRL-ESR-HSP70-PRLR-CoI MMEEAAPP CCGGTCC Broiler
  38. 38. Common-use • DNA in food chain • Biogenetic banks in supporting scientific research • Genomic selection on farm level • Web-based barcode system with food mileage
  39. 39. Acknowledgements • Wu Lab – Mr. Yun-Yu Lai – Dr. Ren-Bao Liaw • – Funding Sponsors Dr. Yuchia Huang – – COA Dr. Ter-Yu Lin – – TLRI Ms. Hsiu-Rong Tsai – Mr. Hsenn-Ben Chu – NAIF • Collaborators – Prof. Hsiu-Luan Chang – Dr. Lih-Ren Chen – Prof. Feng-Pang Cheng – Prof. Larry Schook, UIUC – Prof. Harris Lewin, UIUC – Dr. Le Thi Thuy, Vietnam – Prof. Rangsun Parnpai, Thailand – Dr. Libertado C. Cruz, Philippines – Prof. Chis Maxwell, Australia – Prof. Locksley E. McGann, Canada – Prof. Hoon Taek Lee, Korea

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