Horse Health – Hydrolyzed CollagenWhat is Collagen?                               What is Rousselot ASF?• Collagen is the ...
How is Hydrolyzed Collagen used?                                 Performance Nutrition – Connective Tissue                ...
Hydrolyzed Collagen – Performance Nutrition                      Contains Amino Acids                    critical to maint...
How does Hydrolyzed Collagen Work?                                       Contains Amino Acids                             ...
How is Hydrolyzed Collagen used?                                 Performance Nutrition – Connective Tissue                ...
Hydrolyzed Collagen – Joint HealthCollagen Peptides “Preferentially Accumulate” inCartilageA study by Oesser showed that m...
Hydrolyzed Collagen – Joint Health                                                                                        ...
Hydrolyzed Collagen – Osteoarthritis StudyCollagen Peptides Improve Mobility in Osteoarthritic DogsIn 2009 Beynen carried ...
How is Hydrolyzed Collagen used?                                 Performance Nutrition – Connective Tissue                ...
Hydrolyzed Collagen – Gastric Ulcers                   Frequency of Gastric Ulcers in Horses                              ...
Hydrolyzed Collagen – Gastric Ulcers                                                                                      ...
Hydrolyzed Collagen – Gastric Ulcers                                                                                      ...
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Horse Health - Joint and Gastrointestinal

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This is a brief presentation about Hydrolyzed Collagen, which summarizes studies on Joint Health, Gastrointestinal Health and the incidence of ulcers in horses.

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Horse Health - Joint and Gastrointestinal

  1. 1. Horse Health – Hydrolyzed CollagenWhat is Collagen? What is Rousselot ASF?• Collagen is the most abundant protein • Rousselot ASF is Hydrolyzed Collagen in the body of mammals (25 – 30%). (Hydrolyzed Gelatin).• It is the main structural component of connective tissue (skin, cartilage, • Rousselot ASF contains collagen peptides of ligaments, tendons and bones). low molecular weight (2 – 5,000 Da).• It provides connective tissue with its • Rousselot ASF is easily digestible and ability to withstand great force and metabolizable. stress without breaking down or tearing. • Rousselot ASF has been shown to have a unique and varied role in maintaining the• As people and animals age, the ability health of connective tissue. of the body to produce and maintain collagen progressively declines shortly after reaching adulthood. 1
  2. 2. How is Hydrolyzed Collagen used? Performance Nutrition – Connective Tissue • Collagen Peptides Preferentially Accumulate in Cartilage • Collagen Peptides Induce Production of Type II Collagen • Collagen Peptides Reduce the Production of Catabolic Enzyme • Collagen Peptides Reduce Catabolic Activity in Cartilage • Osteoarthritis Study with Dogs • Summary Hydrolyzed Collagen is used in joint, ligament,tendon, ulcer, GI Health and performance recovery products. Gastrointestinal Health • Frequency of Ulcers in Horses • Recreational Horse Study • Effect on Gastric Lesions • Reduction in Platelet Aggregation • Chemotactic Attraction of Fibroblasts • Summary 2
  3. 3. Hydrolyzed Collagen – Performance Nutrition Contains Amino Acids critical to maintaining and repairing connective tissue. 3
  4. 4. How does Hydrolyzed Collagen Work? Contains Amino Acids critical to maintaining and Amino Acids % Predigested Collagen repairing connective Di- and Tri-Peptides of Protein tissue. (2,000 – 5,000 Dalton) Glycine 20 Hydroxyproline 12 Proline 13Rapidly absorbed intobloodstreamPreferentially accumulates in Chemotactic Effectconnective tissue for on collagenextended period of time. producing cells 80 fibroblasts/20 OIF Chemotactic activity, 60 40 20 0 Control Test 4
  5. 5. How is Hydrolyzed Collagen used? Performance Nutrition – Connective Tissue • Collagen Peptides Preferentially Accumulate in Cartilage • Collagen Peptides Induce Production of Type II Collagen • Collagen Peptides Reduce the Production of Catabolic Enzyme • Collagen Peptides Reduce Catabolic Activity in Cartilage • Osteoarthritis Study with Dogs • How Tendons Work Hydrolyzed Collagen is used injoint, ligament, tendon, ulcer, GI Health and performance recovery products. Gastrointestinal Health • Frequency of Ulcers in Horses • Recreational Horse Study • Effect on Gastric Lesions • Reduction in Platelet Aggregation • Chemotactic Attraction of Fibroblasts • Summary 5
  6. 6. Hydrolyzed Collagen – Joint HealthCollagen Peptides “Preferentially Accumulate” inCartilageA study by Oesser showed that more than 95% of C-labeled collagen peptides taken orally are absorbedand accumulated in the cartilage of mice as early as 12hours after ingestion. The radioactivity was twice ashigh in the group receiving hydrolyzed collagen vs. thecontrol group, demonstrating that collagen peptides“preferentially accumulate” in cartilage. Collagen Peptides Induce Production of Type II Collagen (Cartilage) Another study showed that applying collagen peptides to the cartilage producing cells (chondrocytes) lead to a dose-dependent production of Type II Collagen, reinforcing collagen’s role in the maintenance of connective tissue. 6
  7. 7. Hydrolyzed Collagen – Joint Health 40Collagen Peptides Reduce Blood Concentrations of 35 ng/ml of MMP-3Catabolic Enzyme 30 25In a study by Weide (2004) where hydrolyzed collagen 20 Before 15was fed to dogs, there were corresponding increases 10 Afterin the blood concentration of glycine, proline and 5hydroxyproline, as well as decreases in the enzyme 0MMP-3, which is believed to be the key enzymeinvolved in cartilage matrix degradation. Dogs w/ Joint Problems Healthy Dogs 250 Collagen Peptides Reduce Catabolic Activity in Cartilage 200 µmol/dl of Keratan Sulphate In a study by Appelt 2005 hydrolyzed collagen was 150 Fed Hydrolyzed Gelatin fed to horses over a period of 10 weeks, with Keratan Sulphate being periodically measured during 100 Control Group the study. Sulphate is released during the breakdown of proteoglycans in cartilage, and are a 50 marker of catabolic activity in cartilage. 0 Week 0 Week 5 Week 10 7
  8. 8. Hydrolyzed Collagen – Osteoarthritis StudyCollagen Peptides Improve Mobility in Osteoarthritic DogsIn 2009 Beynen carried out a double blind placebo-controlled trial with privately owned osteoarthritic dogs(N=30). During 8 weeks the dogs daily received eitherRousselot ASF or a placebo (Soy Protein Hydrolysate) as atop dressing over their meal. Dog owners were asked toscore their dogs every 8 weeks on a scale of 0 to 10 fordifferent criteria. Dogs receiving Rousselot ASF showedsignificant improvements in activity, stiffness and lamenees. Potential Mechanisms for Supporting Joint Health • Preferential Accumulation in Cartilage • Collagen Peptides Stimulate Type II Collagen Growth • Reduce Blood Concentrations of Catabolic Enzyme • Reduce Catabolic Activity in Cartilage 8
  9. 9. How is Hydrolyzed Collagen used? Performance Nutrition – Connective Tissue • Collagen Peptides Preferentially Accumulate in Cartilage • Collagen Peptides Induce Production of Type II Collagen • Collagen Peptides Reduce the Production of Catabolic Enzyme • Collagen Peptides Reduce Catabolic Activity in Cartilage • Osteoarthritis Study with Dogs • How Tendons Work • Tendon & Ligament Injuries and Healing Process • Summary Hydrolyzed Collagen is used injoint, ligament, tendon, ulce r, GI Health and performance recovery products. Gastrointestinal Health • Frequency of Ulcers in Horses • Recreational Horse Study • Effect on Gastric Lesions • Reduction in Platelet Aggregation • Chemotactic Attraction of Fibroblasts • Summary 9
  10. 10. Hydrolyzed Collagen – Gastric Ulcers Frequency of Gastric Ulcers in Horses % of Horses with Ulcer 90 80 In 2005 Pellegrini did a large-scale necroscopic study of 70 both recreational and performance horses. The results 60 were published in the Journal of Equine Veterinary 50 Science. There were 365 horses in the recreational 40 group and 180 horses in the performance group. 55% of 30 the recreational horses and 87% of the performance 20 horses were found to have gastric ulcers. 10 0 Recreational Performance Recreational Horse Study 7 In 2005 McClure did a study observing the the effect that# of Horses with Ulcer 6 weekend show travel can have on a horse’s gastro-intestinal 5 health. There were 10 horses in both the control and test 4 group. Endoscopic analysis was used to determine that all horses in the study had no gastric ulcers at the beginning of 3 the study. The control group remained on-site, while the test 2 group was transported 4 hours away by trailer on Day 1, then 1 returned back to original site on Day 4. 0 Control Test 10
  11. 11. Hydrolyzed Collagen – Gastric Ulcers 30 Effect of Hydrolyzed Collagen on Gastric Lesions 25 Damage area, mm2 20 In a study by Samonina in 2000, Hydrolyzed Collagen’s effect on gastric 15 lesions was measured. Gastric mucosal damage was induced in live rats by 10 oral administration of ethanol. For seven days the rats were fed a supplement containing Hydrolyzed Collagen, while the control group was 5 fed their usual diet. At the end of the study, the gastric lesions of all the 0 mice were measured. Control Test Test group given Hydrolyzed Collagen for 7 days 10 Hydrolyzed Collagen – Platelet Aggregation 8Platelet Aggregation 6 In the same study by Samonina, platelet aggregation was measured Index in the blood plasma. Platelet aggregation is the clumping together of 4 platelets in the blood, and is part of the sequence leading up to the 2 formation of clots in the blood. Reduced platelet aggregation is one of the factors which improves microcirculation, which improves 0 gastric mucosal blood flow. Control Test Test group given Hydrolyzed Collagen for 10 days 11
  12. 12. Hydrolyzed Collagen – Gastric Ulcers 70Fibroblast Chemotactic Response to Hydrolyzed 60 OIF activity, fibroblasts/20 ChemotacticCollagen 50 40In a study by Postlethwaite in 1976, collagen producing cells 30(fibroblasts) were shown to have a chemotactic response tohydrolyzed collagen. Chemotaxis is when organisms direct their 20movement according to certain chemicals in their environment. In 10the experiment, fibroblast migration was measured within in vitro 0chemotaxis chambers. Control Test Fibroblast activity measured after 150 minutes of incubation in the chamberMechanisms for Supporting Gastric Mucosal Health• Essential building blocks for maintenance and repair of connective tissue.• Reduces blood plasma levels of Gastrin (Castro 2010). Gastrin is the peptide hormone which stimulates acid secretion in the stomach.• Reduces blood plasma platelet aggregation, improving microcirculation, improving gastric mucosal blood flow.• Induces chemotactic response on collagen producing cells (fibroblasts). 12

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