sensit   TB MPT 64Antigen Rapid test         ubio Biotechnology Systems Pvt Ltd, Cochin                 © 2008 ubio. All r...
TB one-third of the worlds population is currently infected with  the TB bacillus An estimated 1.7 million people died f...
TB Diagnostics Antibody Screening tests are discontinued due to poor reliability. Currently available tests include   • ...
TB Antigen rapid TestsMPT 64TB LAM Test          © 2008 ubio. All rights reserved.   4
About MPT64 The MPT64 antigen is the secretory  protein (24 kDa) that is one of the  secreted major antigens from  tuberc...
Advantages of MPT 64 testDifferentiation between M. tuberculosis and NTMResults in 20 minutes.No need of PCR or Biochem...
MPT 64 Rapid test Sensit TB Ag MPT64 Rapid Test Kit is a  qualitative Immunochromatographic  assay for the detection M. t...
Test Principle Sensit TB Ag MPT64 Rapid Test works on chromatographic  immunoassay. B Basic components of test strip inc...
Sample Preparation Liquid Cultures: One drop of liquid culture can  be directly added to the sample well of the test  dev...
Test Procedure Take out the test card from the foil pouch and place it  on a horizontal surface. Add 25 ul (using pipett...
Test Result Interpretation          © 2008 ubio. All rights reserved.   11
For further details and Orderingubio Biotechnology Systems Pvt LtdXII-111-E/F, Technology Incubation CentreKINFRA Hi-Tech ...
www.ubio.incontact@ubio.in   © 2008 ubio. All rights reserved.   13
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Sensit MPT 64 TB Antigen rapid test

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Sensit MPT 64 TB Antigen rapid test

  1. 1. sensit TB MPT 64Antigen Rapid test ubio Biotechnology Systems Pvt Ltd, Cochin © 2008 ubio. All rights reserved. 1
  2. 2. TB one-third of the worlds population is currently infected with the TB bacillus An estimated 1.7 million people died from TB in 2009(WHO) Africa and Asia account for 65% of total TB cases 3.2% of the worlds estimated new tuberculosis cases were MDR-TB, In India 3% of New TB cases are MDR TB MDR-TB cases in India represent over 20% of the global burden of MDR-TB, with an estimated 131,000 cases in 2007 WHO estimates over 500,000 cases of multidrug-resistant tuberculosis (MDR-TB) occur annually worldwide © 2008 ubio. All rights reserved. 2
  3. 3. TB Diagnostics Antibody Screening tests are discontinued due to poor reliability. Currently available tests include • Sputum Microscopy • Tuberculin test • Interferon Gamma Assay • PCR • TB antigen tests © 2008 ubio. All rights reserved. 3
  4. 4. TB Antigen rapid TestsMPT 64TB LAM Test © 2008 ubio. All rights reserved. 4
  5. 5. About MPT64 The MPT64 antigen is the secretory protein (24 kDa) that is one of the secreted major antigens from tuberculosis bacteria, MPT64 has been shown to differentiate the MTC from other bacterial species © 2008 ubio. All rights reserved. 5
  6. 6. Advantages of MPT 64 testDifferentiation between M. tuberculosis and NTMResults in 20 minutes.No need of PCR or Biochemical tests.Results as early as 2-3 weeks of culture.Low cost and no need of equipments © 2008 ubio. All rights reserved. 6
  7. 7. MPT 64 Rapid test Sensit TB Ag MPT64 Rapid Test Kit is a qualitative Immunochromatographic assay for the detection M. tuberculosis complex in liquid and solid culture © 2008 ubio. All rights reserved. 7
  8. 8. Test Principle Sensit TB Ag MPT64 Rapid Test works on chromatographic immunoassay. B Basic components of test strip includes: a) Conjugate pad, which contains Monoclonal Anti-MPT64 antibody, colloidal gold conjugated; b) a nitrocellulose membrane strip containing two lines a) T: Monoclonal Anti-MPT64 antibody and b) C: Goat Anti Mouse. © 2008 ubio. All rights reserved. 8
  9. 9. Sample Preparation Liquid Cultures: One drop of liquid culture can be directly added to the sample well of the test device. If the culture is too turbid, adjust the opacity with McFarland No 1 using PBS. · Solid cultures: Pick 3-4 colonies from solid culture and suspend in 200 ul of Assay buffer. Treat the specimen as infectious and handle with standard biosafety measures. © 2008 ubio. All rights reserved. 9
  10. 10. Test Procedure Take out the test card from the foil pouch and place it on a horizontal surface. Add 25 ul (using pipette)/one drop (using dropper provided) of the liquid or solid culture (processed as above) to the sample well, followed by the addition of one drop of assay diluent. Wait for 20 minutes and interpret results. The result is considered invalid after 30 minutes. All results where control band does not appear are considered invalid. © 2008 ubio. All rights reserved. 10
  11. 11. Test Result Interpretation © 2008 ubio. All rights reserved. 11
  12. 12. For further details and Orderingubio Biotechnology Systems Pvt LtdXII-111-E/F, Technology Incubation CentreKINFRA Hi-Tech Park, KalamasseryCochin, Kerala, India 683503Ph: +91-9744122269, +91-484-2532966http://www.ubio.in © 2008 ubio. All rights reserved. 12
  13. 13. www.ubio.incontact@ubio.in © 2008 ubio. All rights reserved. 13

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