Transcription ppt BIOCHEMISTRY

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Transcription ppt BIOCHEMISTRY

  1. 1. TRANSCRIPTION Dr. Vijay Marakala, MBBS, MD. Assistant professor BIOCHEMISTRY SIMS & RC
  2. 2. TRANSCRIPTION Definition Similarity and Differentiation Basic requirements Stages Post- transcriptional processing
  3. 3. TRANSCRIPTION [RNA SYNTHESIS] Synthesis of RNA using DNA as a template
  4. 4. TRANSCRIPTION mRNA rRNA tRNA SnRNA
  5. 5. TRANSCRIPTION Similarities • 3 stages • Synthesis occurs in the 5’→3’ direction • Follows Watson-Crick base pairing Differences • Ribonucleotides are used in RNA synthesis • Uracil replaces Thymine • Primer is not required • Only a very small portion of genome is transcribed
  6. 6. TRANSCRIPTION BASIC REQUIREMENTS Template Substrate Enzyme
  7. 7. TRANSCRIPTION
  8. 8. TRANSCRIPTION ATP GTP CTP UTP
  9. 9. TRANSCRIPTION - Basic requirements -ENZYME DNA dependent RNA polymerase or RNA polymerase Three RNA polymerase RNAP-I RNA-II RNA-III EUKARYOTIC Single RNA polymerase PROKARYOTIC
  10. 10. TRANSCRIPTION PROKARYOTIC RNA POLYMERASE
  11. 11. TRANSCRIPTION - STAGES INITIATION ELONGATION TERMINATION
  12. 12. TRANSCRIPTION – STAGES: PROMOTOR REGION
  13. 13. TRANSCRIPTION – STAGES:
  14. 14. TRANSCRIPTION – STAGES:
  15. 15. TRANSCRIPTION – STAGES:
  16. 16. TRANSCRIPTION – STAGES:
  17. 17. TRANSCRIPTION-STAGES:
  18. 18. TRANSCRIPTION-STAGES:
  19. 19. TRANSCRIPTION-STAGES:
  20. 20. TRANSCRIPTION-STAGES: Rho- dependent termination Rho- independent termination 2 mechanisms
  21. 21. TRANSCRIPTION-STAGES: 2 mechanisms
  22. 22. TRANSCRIPTION-STAGES: 2 mechanisms
  23. 23. TRANSCRIPTION IN EUKARYOTES Main differences RNA Polymerase Promoter sites Enhancers, Silencers and TF
  24. 24. TRANSCRIPTION IN EUKARYOTES RNA polymerase-I RNA polymerase-II RNA polymerase-III EUKARYOTIC RNA POLYMERASE
  25. 25. TRANSCRIPTION IN EUKARYOTES
  26. 26. TRANSCRIPTION IN EUKARYOTES ENHANCER

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