DNA replication ppt BIOCHEMISTRY

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DNA replication ppt BIOCHEMISTRY

  1. 1. - - Dr. Vijay Marakala M.D Biochemistry SIMS&RC
  2. 2. CONTENTS Central Dogma Mechanisms Basic requirements Stages Inhibitors - -
  3. 3. RNA PROTEIN DNA Central Dogma - -
  4. 4. - -
  5. 5. DNA structure - -
  6. 6. Semi- conservative mechanism - -
  7. 7. - -
  8. 8. - - Basic requirements Requirements Substrates Template Enzymes Primer
  9. 9. Requirements - - Basic requirements Substrates • dATP dGTP dCTP dTTP
  10. 10. Requirements - - Basic requirements TEMPLATE
  11. 11. - - ENZYMES DNA A Protein DNA Helicase Primase DNA Polymerase SSB DNA Topoisomerase DNA Ligase Ter binding protein Enzymes and Proteins
  12. 12. STAGES Initiation Elongation Termination - -
  13. 13. Unwinding and formation of replication fork - - Replication bubble
  14. 14. - - DNA Replication is Bidirectional
  15. 15. - - Helicase unwinds DNA
  16. 16. - - 5’ 3’ 5’ Primase synthesizes PRIMER Single stranded binding protein
  17. 17. - - 5’ 5’ 3’ 3’ 5’ 3’ Parental DNA Leading strand Laging strand 5’ 3’ 5’ Okazaki fragments Primer Gap filled byDNA Ligase Elongation by DNA Polymerase IIIPrimer is removed by DNA Polymerase I
  18. 18. - -
  19. 19. - -
  20. 20. - -
  21. 21. DNA Ligase - -
  22. 22. Termination sequences “Ter” direct termination - -
  23. 23. - -
  24. 24. DNA →DNA Bidirectional Primers • Semiconservative mechanism • Begins at origin • Synthesis5→3’ • Read 3’→5’ • Leading strand • Laging strand - -
  25. 25. Differences between prokaryotic and eukaryotic DNA replication - - Features Prokaryotes Eukaryotes RNA primer length 50 nucleotides 9 nucleotides DNA polymerase I,II,III α,β,γ,δ,ε Number of origins Single Multiple Nucleotide length of Okazaki 1000-2000 nucleotides 200 nucleotides Rate of replication 500 nucleotides/sec 50 nucleotides/sec
  26. 26. - -
  27. 27. - -
  28. 28. - - Nalidixic acid Novobiocin Ciprofloxacin
  29. 29. - - Adriyamycin Etoposide Doxorubicin
  30. 30. - - THANK YOU

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