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Surf stem cell pipeline


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Surf stem cell pipeline

  1. 1. City  College  of  San  Francisco   Stem  Cell  Pipeline   Stem  Cell  and  Cell  Culture  Program  for  High  School  Students  
  2. 2. Stem  Cell  Pipeline  Program  •  Classes  are  designed  for  high  school  students  •  BTEC  5–  seminar  class  •  BTEC  6–  stem  cell  class  •  Students  start  earning  credit  to  cer=ficates   and  AS  degrees  •  Students  are  recruited  by  their  high  schools.  •  High  schools  are  recruited  by  the  community   college.  
  3. 3. What’s  new  •  Classes  offered  at  Aus=n  Community  College  TX   and  Alamance  Community  College  NC  •  New  ar=cula=on  with  Sequoia  &  San  Mateo  High   School  districts  •  Summer  classes  for  distance  schools  •  Curriculum  on  Bio-­‐Link  Clearinghouse  •  Driving  Biotech  programs  at  other  HS  like  Burton   &  Leadership  •  Collabora=on  with  Gladstone  Ins=tutes  for   summer  Biotech  “Boot-­‐camp”  
  4. 4. BTEC  6   Basics  of  Cell  Culture  •  9  weeks  long  •  Class  meets  once  a  week  •  4  hours  each  session  •  Both  lecture  and  laboratory  components  
  5. 5. Introduc5on  to  the  cell  culture  equipment  
  6. 6. •  Basic  cell  culture  techniques:  •  Defros=ng  cells  from  frozen  stocks  •  Feeding  and  passaging  cells  in  culture  •  Coun=ng  cells  and  calcula=ng  concentra=ons  •  Serial  dilu=on  and  pla=ng  cells  •  Freezing  and  storage  •  Experiments:  •  Asep=c  technique  and  serial  dilu=on  prac=ce  •  Count  and  plate  specific  number  of  cells  •  Live  and  dead  cell  iden=fica=on  using  fluorophores  •  Survival  assay  •  Transfec=on  •  Stem  cell  differen=a=on  
  7. 7. Iden5fica5on  of  live  and   Transfec5on  of  cells  by   dead  cells  using  PI  and   pDsRed2-­‐Mito     Calcein  AM  
  8. 8. 0.5   IC50 Survival  Assay  
  9. 9. Differentiation of embryonic stem cells into neurons Pluripotent ESCs ESCs in suspension Week 1- Floating EBs + RA ImmunocytochemistryMap 2 protein (red) in differentiated neurons Week 2-Differentiated neurons
  10. 10. What  is  your  level  of  interest  in  con=nuing  your  educa=on  in  the  fields  of  health  sciences  and/or  biology?   6   5   4  #  of  Students   Pre   3   post   2   1   0   Very high High Moderate Low Very low
  11. 11. Students’  Comments  •  “Personally  I  am  .red  a0er  a  day  of  school.   There  is  only  so  much  lecturing  I  can  take.”  •  “Lectures  were  boring,  but”  •  “Students  want  prac.ce  more  than  lecture  lectures  makes  us  bored.”  Ideas  to  make  lectures  more  interac5ve??  
  12. 12. More  Student  Comments:  •  “It  was  to  see  the  cardiomyocytes  beat.  It’s   amazing  that  we  preEy  much  made  our  own  neurons/   cardiomyocytes.”  •  “I  think  this  class  is  a  very  good  class  experience  to  have.   I  don’t  know  any  other  high  schooler  who  has  seen  fluorescent  cells.”  •  “This  was  an  amazing  experience.”  •  “I  enjoyed  doing  the  labs  and  wish  that  the  class  lasted   longer  so  we  could  do  more  labs.”  •  “I  learned  a  lot.  Should  offer  more  classes  for  high  school   students.”  
  13. 13. Our  Partners  •  San  Francisco  High  schools:  Lincoln  Wallenberg  Galileo  Leadership  Mission  •  Alamance  Community  College,  NC  •  Aus=n  Community  College,  TX  
  14. 14. Available  Class  materials  on  Bio-­‐Link   Clearinghouse  •  Student  manual  •  Instructor’s  manual  •  Quizzes  and  exams  •  Prac=ce  problem  sets  •  Lectures  
  15. 15. Challenges:   1.  Equal  opportunity  for  students  at   partner  high  schools  (11  high  schools,   8  ISDs)   2.  Poli=cal  climate  toward  human  ES  cells   3.  Student  transporta=on  to  campus  
  16. 16. Challenge:  Picking  par=cipants  Solu=on:  •  Created  a  panel  to  address  logis=cs  :    ACC  representa=ves  and  high  school  teachers  •  Designed  and  implemented  an  applica=on   process  to  select  par=cipants  from  interested   students  Results:  •   Class  of  10  students  and  a  high  school  teacher-­‐   represen=ng  three  high  schools  and  2  ISDs  
  17. 17. Challenge:  Poten=ally  conten=ous  poli=cs  Solu=on:  •  Used  mouse  iPS  cells  rather  than  human  ES   cells  Results:  •  Protocols  are  having  to  be  developed  for  the   course  using  this  alterna=ve  cell  line.  
  18. 18. Challenge:  Transporta=on  Solu=on:  •  Required  students  to  provide  their  own  means  of   transporta=on.    Results:  •   Many  of  the  students  from  lower  socioeconomic   areas  or  from  areas  far  from  the  campus  were  not   able  to  par=cipate.  
  19. 19. Current/Future  work:   1.  Begin  offering  in  the  summer  to  increase   availability.   2.  Develop  an  ebook  for  the  course  to  make   it  more  interac=ve  and  accessible  to  other   schools.   3.  Adapt  the  course  to  be  taught  in  the  high   school  with  a  corresponding  stem  cell   workshop  at  ACC.  
  20. 20. First  Year  •  Recruited  from  5  high  schools  and  the  middle   college  on  ACC  campus  •  11  fall  semester,  8  spring  semester  •  Mix  of  juniors  and  seniors  •  Background  of  basic  algebra  &  biology  
  21. 21. Things  That  Didn’t  Work  •  Extracurricular,  no  school  credit   –  Selling  point:  what  makes  you  stand  out  in  the   field  of  applicants  to  your  school  of  choice  •  Resolu=on:  students  get  a  Cer=ficate  of   Comple=on  •  Future  direc=on:  extend  the  program  to  full   semester  with  more  microbiology,  sterility   basics   –  Becomes  dual  enrollment  with  credit  
  22. 22. Things  That  Didn’t  Work  •  Working  around  two  different  school   schedules,  breaks,  holidays,  etc.   –  Some=mes  2  weeks  between  mee=ngs   –  Cells  need  care  •  Arranged  ac=vi=es  to  allow  for  =me  to   complete  experiments  without  long  breaks  
  23. 23. Things  That  Didn’t  Work  •  Not  able  to  get  ESC  for  the  first  semester  –   numerous  technical  difficul=es   –  Obtained  for  the  second  semester   –  Not  the  same  as  the  mESC  line  used  at  CCSF,  some   results  were  slightly  different   •  No  bea=ng  cardiomyocytes,  did  get  neuron   differen=a=on  
  24. 24. What  Worked  •  Started  with  basics  of  bacteria  (ubiquity),   sterility  and  contamina=on  •  Surprisingly  adept  with  cell  cultures  (CHO-­‐K)  •  Able  to  develop  asep=c  techniques  quickly  •  Successful  ac=vi=es  included:   –  All  but  the  bea=ng  cells  •   Added  a  tour  of  the  Wake  Forest  Ins=tute  for   Regenera=ve  Medicine   –  Mega  hit  with  the  students  
  25. 25. Our  first  partner  high  school:  Lincoln  
  26. 26. Ques=ons?