City College of San Francisco Stem Cell Certification


Published on

Stem Cell Technology Certification at City College of San Francisco.
presenter: Dr. Paul Raju

Published in: Education
  • Be the first to comment

  • Be the first to like this

No Downloads
Total views
On SlideShare
From Embeds
Number of Embeds
Embeds 0
No embeds

No notes for slide

City College of San Francisco Stem Cell Certification

  1. 1. Biotechnology Program <br />Stem Cell Technology Certification<br />Paul A. Raju, D.Phil<br />
  2. 2. Objective<br />Educate and train students with the specialized skills required for jobs in the lucrative and rapidly growing field of stem cell research<br />Launched in 2006<br />
  3. 3. Course Structure<br />BTEC21A: Mammalian Cell Culture <br />(6 weeks/9 hours per week)<br />BTEC21B: Fluorescent Cell Technology <br />(6 weeks/9 hours per week)<br />BTEC21C: Stem Cell Technology <br />(8 weeks/9 hours per week)<br />Pre-requisite - 1 semester of Biology with lab and 2 semesters of Chemistry including Organic with lab<br />BTEC24 & 25: Introductory and Advanced PCR <br />BTEC22: ELISA<br />BTEC23: Western Blotting<br />Text - Culture of Animal Cells: A manual of basic technique (Ian Freshney)<br /> Fall, Spring, and Summer. Day and Night Classes ~1 calendar year to complete<br />
  4. 4. CCSF Cell Culture Facility<br />
  5. 5. Instrumentation<br />2 - Flow Cytometers (BD FACScans)<br />6 - Epifluorscence Inverted Microscopes<br />10 - Inverted Microscopes<br />6 - CO2 Incubators & 6 Laminar Flow Hoods<br />
  6. 6. Main Skills Acquired<br /><ul><li> Understand the principles of stem cell biology
  7. 7. Critically and independently assess the reality and future </li></ul> of applied stem cell biology<br /><ul><li> Differentiate between different types of stem cells in terms </li></ul> of their function, use and care<br /><ul><li> Isolate mesenchymal and hematopoietic stem cells from </li></ul> mouse bone marrow<br /><ul><li> Culture and differentiate mouse Embryonic Stem Cells
  8. 8. Understand (principles) and operate a flow cytometry
  9. 9. Analyze flow cytometry data</li></li></ul><li>Internship Training - in various stem cell research labs in the SF Bay Area (UCSF, Genentech)To date, over 150 students have received their certificatesApproximately half of our graduates have been hired at UCSF, Stanford, Gladstone Institutes, Stem Cell Inc., Geron, Progenitor, Genentech, etc<br />
  10. 10. Evolution of the Program<br />2002 NSF-ATE funded “Fix a Gene” <br />2004 Prop 71 - $3 billion for Stem Cell Research <br />( California Initiative for Regenerative Medicine, CIRM)<br />2005 WIA CA Governor’s grant<br />2005 Creation of Cell Culture Laboratory<br />2006 Launching of Stem Cell Certificate Program<br />2007 First human ES cell training at UCSF<br />2009 CIRM Grant <br />
  11. 11. A Novel Curriculum for Training in Stem Cell Research<br />NSF CCLI Grant 0920799: Workforce Development in STEM Cell Research<br /> Christopher Thomas Scott, Stanford University, PI<br />Aims<br /> Develop a multidisciplinary undergraduate curriculum for community and four year colleges for workforce training in stem cell research. <br /> Cross-disciplinary design: basic and applied science, lab skills, and ethics, law, and policy <br /> Pilot first module in four colleges (SPRING, 2011)<br /> Deploy suite of measurements (summative and formative assessements)<br />
  12. 12. NSF CCLI Grant: Workforce Development in STEM Cell Research<br />Content Developers* and Instructors<br />DritanAgilou* Stanford University<br />Paul AnandaRaju City College of San Francisco<br />Mariluci Blandon Middlesex Community College<br />Jessica Byrne* Art and Illustration<br />KatayounChamany Eugene Lang College<br />Lily Cheng San Francisco State College<br />Eric Chiao* Roche Biosciences<br />Tyler Cutforth* University of California, Santa Cruz<br />Nicole Holthius* Assessment Development<br />Thorsten Schlager* Harvard University<br />Carin Zimmerman* City College of San Francisco<br />
  13. 13. NSF CCLI Grant: Workforce Development in STEM Cell Research<br />Year 1 Progress: > 80 hours of Instruction and Materials<br /> Primers: Short topical introductions for students<br /> Slide Presentations: Fully-illustrated slide sets with teacher’s notes and image library<br /> Activities: Cross-topic, multi-week activities test essential concepts and reflective learning<br /> Integrated Laboratories: Fully immersive labs teaching essential skills in stem cell biology<br /> Assessment Suite: Embedded, formative, and summative measurements <br />
  14. 14. Acknowledgements<br />CCSF<br />Phil Jardim<br />Kristin Hershbell<br />Dr. Carin Zimmerman<br />Dr. GolnarAfshar<br />Dr. Alex Zambon<br />Dr. Jennifer Ng<br />Dr. Edith Kaeuper<br />Dr. Wing Tsao<br />Dr. Carole Toebe<br />Dr. Elaine Johnson<br />UCSF/Gladstone<br />Dr. Mort Cowan<br />Ms. Elizabeth Dunn<br />Dr. Bruce Conklin<br />Dr. Jennifer Ng<br />Dr. Susan Fisher<br />MiltenyiBiotech<br />Mr. Thad Baker<br />Ms. Julie Rae<br />Dr. Shane Oram<br />