Novel Resistance to Rust Pathogens in Bread Wheat

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Li Huang, Montana State University

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Novel Resistance to Rust Pathogens in Bread Wheat

  1. 1. Novel resistance to rust pathogens in bread wheat Li Huang Montana State University
  2. 2. Mutagenesis Alpowa--Susceptible line Leaf rust screening Treat seeds with EMS Select for resistance ones Mutation density: 1/12 kb (Feiz et al., 2009, Genetics)
  3. 3. Al-180 Al-220 Al-268 Al-271 Al-535 Alpowa MNs MNs MNs MNs MNs APRPt S R R R R R S R R R S SPgt R R R R R SPst APR S R R S S S
  4. 4. Al-220‘Alpowa’ MNs
  5. 5. Infection types of the mutant and the wild type with Pt race PBJL 3 dpi 6 dpi Al-220 Al-220 ‘Alpowa’ MNs ‘Alpowa’ MNs
  6. 6. Infection types of the mutant and the wild type with Pt race PBJL at seedling stage Al-220 ‘Alpowa’ MNs dpi 12 15 18 21 12 15 18 21
  7. 7. Infection types with Pt race PBJL at adult stage Al-220 ‘Alpowa’ MNsdpi 18 21 24 18 21 24
  8. 8. Infection types with Pt race PBJL at seedling stage Susceptible Resistance8 11 14 17 20 8 11 14 17 20 Chinese Spring x MNsAl-220
  9. 9. Infection types with Pgt race TMLK at seedling stage Al-220 ‘Alpowa’ MNsdpi 18 21 24 18 21 24
  10. 10. Al-220Infection types of MNs and ‘Alpowa’ with races of three rust pathogens Lines Pathogens Races Stage Al-220 ‘Alpowa’ MNs PBJL Seedling 3~4 ;~2 Adult S R Pt SBGD Adult S I BBBDD Adult S I 78 Seedling 8 7 127 Seedling 8 8 Pst Adult R R 101/102 Adult S R TLMK Seedling 3-~4 ;1+ QFCS 3~4 ;1+ TTKSK 3+ ;1+ TTKST 3+ ;1+ Pgt TTTSK 3+ ; TPMK 4 ; TTTT 4 ;1+ QTHJ 3+ ;1+
  11. 11. Infection types with a local Egt race at seedling stage Al-220 ‘Alpowa’ MNsdpi 18 21 24 18 21 24
  12. 12. Seedling stagePR Genes Enzyme Actvity PR1 PR2 β-1,3-Glucanase PR3 Endochitinase PR4 Endochitinase PR5 Thaumatin-like PR9 Lignin-forming Peroxidase PR10 RNase Adult stage
  13. 13. Al-220MNs‘Alpowa’
  14. 14. Al-220 Genetic analysis of resistance in the MNs F2 individuals Expected Crosses Ratio X2 Resistant Susceptible Al-220 MNs / ‘Alpowa’ 210 71 3:1 0.67 Al-220‘Chinese Spring’ / MNs 100 36 3:1 0.69
  15. 15. 2BS cM CentromereGenetics map of Lr48 Chinese Spring 2B Bin MapBansal et al., 2008, TAG Genetic map of MNsAL220 GrainGenes
  16. 16. AcknowledgementThe Huang Lab Jackie Campbell Dr. Hongtao ZhangCollaborators Dr. Mike Giroux MSU Dr. Yue Jin USDA-ARS, Cereal Disease Lab Dr. Meinan Wang WSU Dr. Xianming Chen WSU Dr. Deven See WSUFunding

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