Sensako Rust Evaluation Strategy

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Francis Koekemoer

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Sensako Rust Evaluation Strategy

  1. 1. SENSAKO RUST EVALUATION STRATEGY Francois Koekemoer South Africa
  2. 2. National breeding programs •  Sensako (private company ± 75% market share) •  Pannar (private company ±7% market share) •  Small Grain Institute – ARC-SGI (para-statal company ± 18% market share)
  3. 3. National pre-breeding programs •  Free State University •  Stellenbosch University •  CenGen •  ARC-SGI
  4. 4. National monitoring program •  ARC-SGI
  5. 5. Wheat  Produc-on  Areas   - Irrigation areas - Dryland Winter wheat - Dryland Spring Wheat
  6. 6. TTKSF  
  7. 7. PDRS   CCPS   MCDS  
  8. 8. New wheat rust races in SA since 2000 2000: P. graminis f.sp. tritici TTKSF (2SA88) Ug99 race group 2005: P. graminis f.sp. tritici BPGSC (2SA105) Virulence for SrSatu in triticale P. triticina (3SA144) Virulence for Lr32 P. striiformis f. sp. tritici 6E22A+ Virulence for YrA 2007: P. graminis f.sp. tritici TTKSP (2SA106) Ug99 race group, virulence for Sr24 2009: P. graminis f.sp. tritici PTKST (2SA107) Ug99 race group, virulence for Sr24+Sr31 P. triticina CCPS (3SA145) Virulence for Lr12+13+37 2010: P. graminis f.sp. tritici TTKSF+ Ug99 race group, virulence for SrWeb P. triticina MCDS (3SA146) Virulence for Lr12+13+37 P. triticina FBPT (3SA147) Virulence for Lr12+13+37
  9. 9. Pre-breeding University of Stellenbosch •  MS-MARS •  Since 1998 •  Specific genes via MAS •  APR •  Distribute rust resistance material –  Nurseries (8 editions since 2006) –  Lines together with molecular info –  Expanded to include replicated plots and 3m rows –  Supply data regarding CIMMYT nurseries
  10. 10. Introduction of new sources •  BC component •  Introduction of new sources of resistance – T. dicoccoides: Lr53/Yr35 (6B) – T. kotschyi: Lr54/Yr37 (2D) – T. sharonense: Lr56/Yr38 (6A) – T. peregrinum (UUSS): Lr59 – T. neglecta: Lr62/Yr42 (6A) •  Facilitated via MAS (and seedling tests)
  11. 11. Distribu1on  of  resistant  material   0   5   10   15   20   25   1   2   3   4   5   6   7   8   9   13   Gene  combina-ons  –  2012  Nursery    
  12. 12. Evaluation and selection strategy •  Obtain sources for resistance •  CIMMYT, Europe, Australia, Argentina, pre-breeding programmes •  Crosses in greenhouse •  Normally one backcross (BC1) •  Double haploids on F1 seeds
  13. 13. Evaluation and selection strategy •  Evaluate advance breeding lines and new DH lines under field inoculated conditions and conduct selections in early generation nurseries –  Stem and leaf rust •  Makhathini (artificially inoculated pathotypes) and Napier (natural occurring pathotypes) –  Stripe rust •  Napier (artificially inoculated pathotypes)
  14. 14. Bethlehem   Johannesburg   Makathini   Napier  
  15. 15. Breeding strategy •  Stem rust - Sr2 as pivot gene combined with other genes •  Leaf Rust – Lr34 as pivot gene combined with other genes •  Stripe rust – Yr18 as pivot gene combines with other genes
  16. 16. Stem rust progress suscep1ble   Sr2  intermediate   Sr2  +  ?  genes   Sr2  +  ?  genes  
  17. 17. Successes and set backs Cultivar Stem Rust Leaf Rust Stripe Rust SST 88 S S MR SST 015 S MS R SST 027 MS MS R SST 047 R R R SST 056 MS MR MR SST 087 S R R SST 096 MR MR MR SST 017 MR MR R SST 0127 MR MR R SST 0137 MR MR MR
  18. 18. Tools to expedite breeding –  Double haploids –  Shuttle breeding –  Marker assisted selection (cost?)
  19. 19. Interaction and stewardship –  Southern African Breeders Association (SAPBA) –  Winter Cereal Trust (WCT) –  Rust Workshop –  Try to avoid releasing single dominant genes –  Increase frequency of various genes in wheat backgrounds
  20. 20. Acknowledgements –  BGRI –  Prof Zakkie Pretorius –  Dr Tarekegne Tarefe –  Mr Willem Botes –  Sensako team

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