Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
Presented by….
BALRAM SOLUNKE
balram2424@gmail.com
Introduction To Bio
Control
Laboratory
Location of BCL:-
 Name of department :-Agril. Entomology
 Name of college :- K. K. Wagh college of agril.
Nashik-03
 N...
External view of bio control
laboratory
Experiential learning Module Activity
Course No. AEL-ENT-486
Title : Mass production of bioagent and biopesticides
Credits...
No. of cells of Biocontrol lab :-
1) Sterilization room
2) Media room
3) Inoculation room
4) Growth room 1
5) Growth room ...
STERILIZATION ROOM :
1) use of this room for
sterilization through the
Autoclave.
2) This room should be
well ventilated b...
For maximum
flexibility, autoclave
space is
recommended on
each floor, or at a
minimum in a
convenient location
in each bu...
Media preparation room :
This room should get a sufficient & continuous
supply of single & double-distillation unit ,press...
Inoculation room :
Inoculation room :
1) In this room the inoculation cabinet laminar air flow can be
kept.
2) The floor of this room should ...
GROWTH ROOM :
GROWTH ROOM :
1)In this room all inoculated bottles with spores
are kept.
2)This room should be in sterile condition
3)Air...
STORE ROOM :
1) In this room store in Chemicals
2) Then Chemicals are Agar agar,
ethyl alcohol, peptone,
streptomycine, ta...
MIXING ROOM
1) Grinding of different
fungus like
trichoderma,verticilium,
beauveria,metarhizium
etc.
2) Mixing the myceliu...
INSECT REARING ROOM :
1) In this room rearing of
helicoverpa larvae &
spodoptera .
2) Rearing of moth of
helicoverpa & spo...
 MICROVAVE OVEN :
 Microwave oven use to heat the content of
artificial diet.
NPV ROOM :
1) This room is also called as virus
room.
2) All viral processed taken in this
room.
3) NPV made in this room....
WASHING ROOM :
1) The room should be
provided with necessary
service lines as water
,cleaning materials i.e.
soap, sodium ...
THANK YOU
Introduction to bio control lab for the production of bio pesticide and bio agents.
Introduction to bio control lab for the production of bio pesticide and bio agents.
Upcoming SlideShare
Loading in …5
×

Introduction to bio control lab for the production of bio pesticide and bio agents.

3,181 views

Published on

Introduction to bio control lab for the production of bio pesticide and bio agents.

Published in: Education

Introduction to bio control lab for the production of bio pesticide and bio agents.

  1. 1. Presented by…. BALRAM SOLUNKE balram2424@gmail.com
  2. 2. Introduction To Bio Control Laboratory
  3. 3. Location of BCL:-  Name of department :-Agril. Entomology  Name of college :- K. K. Wagh college of agril. Nashik-03  Name of farm area:- Puriya park, Saraswati nagar Panchvati Nashik-03  Name of university :- M.P.K.V ,Rahuri
  4. 4. External view of bio control laboratory
  5. 5. Experiential learning Module Activity Course No. AEL-ENT-486 Title : Mass production of bioagent and biopesticides Credits=0+20 Major Department: Agril. Entomology Associated subjects: Economics, Extension Education No. of students Registered: 14 Activities carried out by students: Rearing of host insect like Corcyra cephalonica, Mealy bug Rearing of Bioagents like Trichogramma, Cryptolaemus, Zygogrmma etc. Mass production of HaNPV and SlNPV Mass production of Metarhzium, Nomuraea, Verticelium, Beavaria,Trichoderma & Psudomonus etc.
  6. 6. No. of cells of Biocontrol lab :- 1) Sterilization room 2) Media room 3) Inoculation room 4) Growth room 1 5) Growth room 2 6) Store room 7) Mixing room1 8)Mixing room2 9) Insect rearing room1 10) Insect rearing room2 11)Bio agent room1 12)Bio agent room2 13)NPV room 14)Washing room
  7. 7. STERILIZATION ROOM : 1) use of this room for sterilization through the Autoclave. 2) This room should be well ventilated by fitting exhaust fan. 3) In this room should be 2 autoclave, a horizontal & a vertical autoclave & Hot air oven.
  8. 8. For maximum flexibility, autoclave space is recommended on each floor, or at a minimum in a convenient location in each building, where microbiological research is performed.AUTOCLAVE
  9. 9. Media preparation room : This room should get a sufficient & continuous supply of single & double-distillation unit ,pressure cooker , Exhust fan.
  10. 10. Inoculation room :
  11. 11. Inoculation room : 1) In this room the inoculation cabinet laminar air flow can be kept. 2) The floor of this room should be covered with linoleum or tiles to facilitate proper cleaning. 3) It is essential that the atmosphere in this room should be free from contaminants. 4) This room should preferably be air-conditioned because it ensures good air-circulation & helps in maintaining constant temp.
  12. 12. GROWTH ROOM :
  13. 13. GROWTH ROOM : 1)In this room all inoculated bottles with spores are kept. 2)This room should be in sterile condition 3)Air condition should be there to control temperature. 4)The following fungi are grow in this room : a)Verticillium lecanii b)Beauveria bassiana c)Nomuraea rileyi d)Metarrhizium anisopliae e)Trichodemma spp.
  14. 14. STORE ROOM : 1) In this room store in Chemicals 2) Then Chemicals are Agar agar, ethyl alcohol, peptone, streptomycine, talc powder etc. 3) The chemical rack should be arrange with A to Z alphabetical latters so its easy to find chemicals.
  15. 15. MIXING ROOM 1) Grinding of different fungus like trichoderma,verticilium, beauveria,metarhizium etc. 2) Mixing the mycelium culture with talcum powder 3) Drying of blended culture 4) Equipment: laboratory blander two types of microscope I. Steriozoom microscope II. binocular microscope
  16. 16. INSECT REARING ROOM : 1) In this room rearing of helicoverpa larvae & spodoptera . 2) Rearing of moth of helicoverpa & spodoptera moth in glass chamber. 3) Rearing of mealy bug on pumpkin as a food for cryptolamous its use in off season when the mealy bug can not be available.
  17. 17.  MICROVAVE OVEN :  Microwave oven use to heat the content of artificial diet.
  18. 18. NPV ROOM : 1) This room is also called as virus room. 2) All viral processed taken in this room. 3) NPV made in this room. 4) The Infected food i.e. .gram, A.diet given to the helicoverpa larvae given in this room. 5) Equipment : centrifugal, laboratory blender, rack, exhaust, fan & furniture. 6) ventilation system provide in this room for exhaust of dirty smell.
  19. 19. WASHING ROOM : 1) The room should be provided with necessary service lines as water ,cleaning materials i.e. soap, sodium chloride . 2) In two side of the room a washing sink can be fitted. 3) The drainage & drying racks could be accommodated in this room.
  20. 20. THANK YOU

×