Dna replication review for matching worksheet final

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Dna replication review for matching worksheet final

  1. 1. By: Monique Hernandez& Trevor Dunbar
  2. 2. Why does DNA Replicate? To make an identical copy of DNA for cell division in Mitosis andMeiosis Meiosis is a specialized cell division process for sexualreproduction Cell division for cells other than sex cells, is known as Mitosis
  3. 3. First Step: Open Up the Double Helix Where: Origin of Replication on DNA strand
  4. 4. Key Players: DNA helicase: A protein that works to “unzip” the DNAand separate the antiparallel strands Single stranded binding proteins: keep the newly singlestranded DNA stable and work to prevent hairpin heliceson lagging strand
  5. 5. How do chromosomes not gettightly tangled? Topoisomerase 1: works to relieve tension made inDNA strand by making a break in the backbone of asingle strand. Topoisomerase 2: works to relieve tension during the“unzipping” of DNA by making a double strandedbreak. http://www.youtube.com/watch?v=EYGrElVyHnU
  6. 6. Second Step: Prime the DNA Strand Leading Strand: Strand on which there is continuoussynthesis of DNA http://www.youtube.com/watch?v=dsoOPDgV0wc
  7. 7. Leading Strand Key Players: Primase will add an RNA primer to the DNA strand. DNA polymerase will bind to the RNA primer andbegin synthesizing DNA in the 5’3’ direction The clamp protein binds to DNA polymerase andprevents it from dissociating from the DNA templatestrand
  8. 8. Lagging Strand Discontinuous synthesis of DNA; backstitches in the5’3’ direction The segments synthesized by DNA polymerase arecalled “Okazaki fragments” http://www.youtube.com/watch?v=H_l0rnvPcTA
  9. 9. Lagging Strand Key Players: Every ~100 nucleotides, RNA primers are added by aDNA primase DNA polymerase will let go of DNA once it meets thenext RNA primer Exonuclease will recognize RNA primer and cut it out. DNA polymerase will fill in the missing gap withnucleotides DNA ligase will seal the nicks in the strand

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