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ANGIOFLUORESCEINOGRAFIA_PARTE 1

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ANGIOFLUORESCEINOGRAFIA_PARTE 1

  1. 1. PRINCIPIOS DE LAANGIOGRAFÍA CON FLUORESCEÍNA (Parte I) Carlos Azañero Inope Residente - Oftalmología
  2. 2. PRINCIPIOS DE LA ANGIOGRAFÍA CON FLUORESCEÍNAFluorescence is a form ofluminescence, or cold-bodyradiation, characterized by the excitationof a material with a high energy (shortwavelength) photon with subsequentrelease of a lower energy (longerwavelength) photon. Dr.Carlos Augusto Azañero Inope www.carlosvirtual.com
  3. 3. PRINCIPIOS DE LA ANGIOGRAFÍA CON FLUORESCEÍNASodium fluorescein, NaC20H12O5, is ahydrocarbon that is excited bywavelengths from 465 to 490 nm (blue)and emits light at a wavelength of 520530 nm (green–yellow) Dr.Carlos Augusto Azañero Inope www.carlosvirtual.com
  4. 4. PRINCIPIOS DE LA ANGIOGRAFÍA CON FLUORESCEÍNA Dr.Carlos Augusto Azañero Inope www.carlosvirtual.com
  5. 5. PRINCIPIOS DE LA ANGIOGRAFÍA CON FLUORESCEÍNASuccessful fluorescein angiography relies upon two interferencefilters, which allow only a narrow bandwidth of light to pass, anexcitation and a barrier filter. Dr.Carlos Augusto Azañero Inope www.carlosvirtual.com
  6. 6. PRINCIPIOS DE LA ANGIOGRAFÍA CON FLUORESCEÍNASodium fluorescein is a low molecularweight molecule (367 Da) and readilydiffuses through the fenestrated vesselsof the choriocapillaris, but does notpass through healthy endothelial cellsof nonfenestrated retinal vessels orthrough the retinal pigment epithelium(RPE); this property is usefuldiagnostically. Dr.Carlos Augusto Azañero Inope www.carlosvirtual.com
  7. 7. PRINCIPIOS DE LA ANGIOGRAFÍA CON FLUORESCEÍNAIn humans, only one- fifth of sodiumfluorescein remains unbound in thebloodstream and is available forimaging; the rest remains protein-bound, primarily to albumin. Dr.Carlos Augusto Azañero Inope www.carlosvirtual.com

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