2009StanfordE25-Alex Avery- PCR video

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2009StanfordE25-Alex Avery- PCR video

  1. 1. Polymerase Chain Reaction: Just Another High School Memory By Alex Avery
  2. 2. Introduction This slide show will hopefully help students remember the always confusing process of PCR, or polymerase chain reaction. Underlined words in my brief high school drama are key words that represent key steps in the scientific process of PCR. I hope this can be used as one more tool to help students out in years to come.
  3. 3. Ingredients  DNA Template = High School Class  DNA Primers = First School Dance  Taq Polymerase = Usher’s “Nice & Slow”  Nucleotides-A, T, G, C = Students
  4. 4. Base Pairing Athletic = Tall QuickTimeª and a TIFF (Uncompressed) decompressor are needed to see this picture.
  5. 5. Base Pairing Tattooed = Artsy QuickTimeª and a TIFF (Uncompressed) decompressor are needed to see this picture.
  6. 6. Base Pairing Charming = Girly
  7. 7. Base Pairing Gentlemen = Christian
  8. 8. Step 1 This step does not work with my metaphor, so you are going to have to simply remember: Mix ingredients at room temperature (25 degrees Celcius)
  9. 9. Step 2 You show up to your first day of school. Whether you are a rising freshman, sophopmore, junior, or senior, all you have to remember is the word rising, because that is what the temperature is doing in this step. You have all broken off your summer flings and are now single just like the DNA.
  10. 10. Step 2 (cont) In other words Heat to 94 degrees Celcius to melt dsDNA into ssDNA
  11. 11. Step 3 Before going to the dance, you take a shower and put on some deodorant to keep your cool. Only then can you go to the dance.
  12. 12. Step 3 (cont) In other words Cool to 45-65 degrees Celcius to allow primers to anneal to DNA template
  13. 13. Step 4 In 72 minutes, you can play Usher’s hit song “Nice & Slow” 19 times, and by then, you are sure to have maximized student pairing.
  14. 14. Step 4 (cont) In other words Heat to 72 degrees Celcius for best base pairing by Taq polymerase
  15. 15. Repeat Repeating this process doubles the previous amount of dsDNA. Repeat steps until desired amount is produced (usually 30-40 times).

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