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Chromatography
Chromatography
Definition:
“A technique by which we can separate components of a mixture due
to their different distributi...
History of Chromatography
• It was first employed by Russian scientist Mikhail Tsvet in 1900
• It was primarily for the se...
Components
Components of Chromatography:
 Mixture containing Analyte
 Stationary Phase
 Mobile Phase
Principle of Chromatography
A component which is quite soluble in the stationary phase will take
longer to travel through ...
Types of Chromatography
Adsorption Chromatography
It utilizes a mobile liquid or gaseous phase that is adsorbed onto the
s...
Uses of Chromatography
 It can be used in crime scene investigations
 In hospitals it can be used to detect alcohol leve...
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Chromatography

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Chromatography

  1. 1. Chromatography
  2. 2. Chromatography Definition: “A technique by which we can separate components of a mixture due to their different distribution between stationary and mobile phase is called Chromatography” Chromatography, literally meaning "color writing"
  3. 3. History of Chromatography • It was first employed by Russian scientist Mikhail Tsvet in 1900 • It was primarily for the separation of plant pigments such as chlorophyll, carotenes, and xanthophyll • New types of chromatography developed during the 1930s and 1940s made the technique useful for many separation processes • Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s • Advances are continually improving the technical performance of chromatography
  4. 4. Components Components of Chromatography:  Mixture containing Analyte  Stationary Phase  Mobile Phase
  5. 5. Principle of Chromatography A component which is quite soluble in the stationary phase will take longer to travel through it than a component which is not very soluble in the stationary phase but very soluble in the mobile phase
  6. 6. Types of Chromatography Adsorption Chromatography It utilizes a mobile liquid or gaseous phase that is adsorbed onto the surface of a stationary solid phase Partition Chromatography This form of chromatography is based on a thin film formed on the surface of a solid support by a liquid stationary phase. Solute equilibrates between the mobile phase and the stationary liquid
  7. 7. Uses of Chromatography  It can be used in crime scene investigations  In hospitals it can be used to detect alcohol levels in a patient's blood stream  It can be used for environmental agencies to determine the level of pollutants in water supplies  It can be used to purify chemicals needed to make a product in a manufacturing plant  It can be used by pharmacists to determine the amount of each chemical found in each product

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