Citrus

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Citrus

  1. 1. Citrus-A case study<br />Chinju Maria Mathew<br />008315<br />
  2. 2. Citrus…???<br />No 1 : Nutritional value and productivity.<br />Origin : 4000 BC in South-East Asia.<br />Family : Rutaceae (Evergreen Trees)<br />Nature : Acidic.<br />Reservoir : Vitamin C, Vitamin B9 and Dietary Fibers.<br />Phytochemicals : Reduces risk of many Chronic diseases.<br />
  3. 3. Millennium Break Through !!!<br />4 decades of research – Poor Practical Results.<br />Micro grafting.<br />Nucellar Embryogenesis.<br />Shoot Meristem Regeneration.<br />My pick…<br />“ Tissue Culture of Citrus Cultivars”<br />
  4. 4. At a glance…<br />Nuclear Institute for Agriculture and Biology.<br />Kinnow-Mandarin, rough Lemon, Sweet Lime,<br /> Gada-dehi, Washington Navel, Grapefruit.<br />Aims :<br /> Production of healthy and vigorous calli.<br /> Regeneration from calli.<br /> Production of Somatic Hybrids.<br />
  5. 5. In Lab…<br />Explants :<br /> Nucellus, Under developed seeds, Seedling leaf, Basal node and Ovary.<br />Cultured on MS medium, supplemented with<br /> BA + Kn + 2,4-D + NAA + IAA+ (Glutamine/Malt extract)<br />Reciprocal Crosses.<br />Seed Characteristics. <br />
  6. 6. To the world…?<br />Success lies on Explant and Medium.<br />Only 2,4-D induced Callusing.<br />BA + 2,4-D => Good Quality Embryogenic Calli.<br />BA + Kn => Shoot and Root Induction.<br />2,4-D+Kn => High Quality Calli.<br />Callus formation on the 10th day – Creamy White.<br />Sub-culturing turned GREEN and into mature embryo.<br />
  7. 7. To the world…?(Cntd)<br />Maturation directly proportional to Dryness of the Medium.<br />Secondary Embryogenesis and Pseudo bulbils formation.<br />Production of Somatic hybrids.<br />Tendency to fruit drop (Kinnow).<br />Gada-dehi, maximum number of healthy seeds.<br />
  8. 8. My point of view…<br />POSITIVES<br /><ul><li>Explant - Nucellus.
  9. 9. Detailed study of plant growth hormones on Explant
  10. 10. Detailed study of seed characteristics.
  11. 11. Production of SomaticHybrids.
  12. 12. Formation of Pseudobulbils and Secondary Embryogenesis.</li></li></ul><li>My point of view…(Contd.)<br />NEGATIVES<br /><ul><li>Nucellus exhibit the phenomenon of Juvenility and delayedfruiting.
  13. 13. Higher concentration of 2,4-D => Browning of medium.
  14. 14. Explant collected from field grown trees induced Contamination.
  15. 15. Notable difference and its explanation in weight of seeds is missing.
  16. 16. Tendency to fruitdrop in hybridplants.</li></li></ul><li>To sum it up…<br />Tissue culture and micro propagation – an integral part of plant transformation.<br />Production of Citrus increased steadily.<br />Research was indeed successful and they came up with variety of explants.<br />Resulted in the propagation of healthy citrus plants.<br />
  17. 17. References.<br />
  18. 18. Thnkz….<br />

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