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 Monoclonal antibodies are
monospecific , made by
Identical immune cells that are
all clones of unique parent cell.
 Hav...
 Immunization of mouse
 Fusion of myeloma cells and immune spleen
cells
 Culturing in HAT media
 Separation of hybrido...
 Cell ,cell debris ,lipids,
clotted materials are first
removed by
 Charged impurities such
nucleic acids and endotoxins...
 Quick or single step method is
is problematic for
antibodies which can easily damaged.
Whole antibody is of murine origin.
Murine variable region fused onto
human C region.
By grafting murine hyper variable
am...
Biosensors & Microarrays
Cancer , Transplant rejection,
Infectious diseases
Purification of drugs , Imaging
the target
Fig...
NAME TYPE TARGET APPLICATION
OKT3 Murine CD3 molecules Acute rejection
of organ
INFLIXIMAB Chimeric Inhibits TNF-a Rheumat...
When monoclonal antibody attaches to cancer cell :
Rituximab drug attaches to specific
protein CD20 found on B cells makes...
Bevacizumab blocks growth signal
which attract blood vessels then tumor
shrinks.
By combining radioactive particle with
mo...
Monoclonal antibodies
Monoclonal antibodies
Monoclonal antibodies
Monoclonal antibodies
Monoclonal antibodies
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Monoclonal antibodies

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Monoclonal antibodies

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Monoclonal antibodies

  1. 1.  Monoclonal antibodies are monospecific , made by Identical immune cells that are all clones of unique parent cell.  Have monovalent affinity, They bind to same epitope.
  2. 2.  Immunization of mouse  Fusion of myeloma cells and immune spleen cells  Culturing in HAT media  Separation of hybridoma cells  Cloning of hybridoma cells
  3. 3.  Cell ,cell debris ,lipids, clotted materials are first removed by  Charged impurities such nucleic acids and endotoxins seperated by
  4. 4.  Quick or single step method is is problematic for antibodies which can easily damaged.
  5. 5. Whole antibody is of murine origin. Murine variable region fused onto human C region. By grafting murine hyper variable amino acid domains into human antibody.
  6. 6. Biosensors & Microarrays Cancer , Transplant rejection, Infectious diseases Purification of drugs , Imaging the target Fight against Bioterrorism
  7. 7. NAME TYPE TARGET APPLICATION OKT3 Murine CD3 molecules Acute rejection of organ INFLIXIMAB Chimeric Inhibits TNF-a Rheumatoid arthritis OMALIZUMAB Humanized IgE Moderate to serve allergic asthma PALIVIZUMAB Humanized Inhibits an RSV fusion protein RSV infection in children
  8. 8. When monoclonal antibody attaches to cancer cell : Rituximab drug attaches to specific protein CD20 found on B cells makes cell more visible to immune system Cetuximab attaches to growth factor receptors , blocking its signal and suppress cancer growth
  9. 9. Bevacizumab blocks growth signal which attract blood vessels then tumor shrinks. By combining radioactive particle with monoclonal antibodies, radiation are deliver to cancer cells only , cancer cell dies.

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