Study of anti-microbial activity of
Hibiscus rosacinensis leaf extract.
-Arijit Chakraborty
Project done under supervision...
Theory:
Anti-microbrial:
An anti-microbial is a substance that kills
or inhibits the growth of microorganisms[1] such
as b...
• Synthetic agents:
– Sulphonamides, cotrimoxazole, quinolones
– Anti-virals
– Anti-fungals
– Anti-cancer drugs
– Anti-mal...
 Apparatus required:
i) Round bottom flask (RBF)
ii) Socks let apparatus.
iii) Beaker (1000 ml, 500 ml, 250 ml)
iv) spatu...
Procedure of Solubility chake:
8 test tubes are taken. 4 of them cold
water and rest are hot water.
Procedure of Thin Laye...
Procedure:
I. Leaves are collected and dried in room
temperature up to one week.
II. Then leaves are crushed in mixing gri...
VIII. Extraction sample are taken in to petridishs.
IX. Then dried the Petridis.
X. Then chake the solubility of the extra...
Result
 Observation table of solubility:
Sample Cold water Hot water
Ethanol extract Soluble Soluble
Chloroform extract I...
Observation table of TLC:
Sample Response
Ethanol extract + ve
Chloroform extract -Ve
PET-Ether extract -Ve
Water extract...
Observation table of R.W.C:
Concentration Time Of contact (gm)
2(1/2) 5 7(1/2) 10
1/100
(phenol)
+ + - -
1/100 (ethanol) ...
Concentration Time Of Contact (gm)
2(1/2) 5 7(1/2) 10
1/100 (phenol) + + - -
1/100 (water) + + + -
1/200 (water) + + - -
1...
 Calculation:
Formula:
Rideal walker coefficient= (MIC of phenol/ MIC of
sample)
Ethanol extraction:
R.W.C = (MIC of phen...
 Conclusion:
After performing the experiment, it
was found that Rideal walker coefficient of
ethanol extraction is 1 and ...
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Study of anti microbial activity of hibiscus rosacinensis leaf

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Study of anti microbial activity of hibiscus rosacinensis leaf

  1. 1. Study of anti-microbial activity of Hibiscus rosacinensis leaf extract. -Arijit Chakraborty Project done under supervision of Biswanath Ghosh
  2. 2. Theory: Anti-microbrial: An anti-microbial is a substance that kills or inhibits the growth of microorganisms[1] such as bacteria, fungi, or protozoan's. Antimicrobial drugs either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects or outside the body. Main classes: There are mainly two classes of antimicrobial drugs: Those obtained from natural sources:  Beta-lactam antibiotic (such as penicillins, cephalosporins) Protein synthesis inhibitors (such as aminoglycosides, macrolides, tetracyclines, chloramph enicol, polypeptides)
  3. 3. • Synthetic agents: – Sulphonamides, cotrimoxazole, quinolones – Anti-virals – Anti-fungals – Anti-cancer drugs – Anti-malarials – Anti-tuberculosis drugs – Anti-leprotics • Anti-protozoals
  4. 4.  Apparatus required: i) Round bottom flask (RBF) ii) Socks let apparatus. iii) Beaker (1000 ml, 500 ml, 250 ml) iv) spatula v) Glass rod vi) Funnel  Chemicals required: i) Ethanol ii) Chloroform iii) Pet ether iv) Phenol v) Peptone, Nacl, Beef extract vi) Distilled water
  5. 5. Procedure of Solubility chake: 8 test tubes are taken. 4 of them cold water and rest are hot water. Procedure of Thin Layer Chromatography: At first TLC plate are prepared by silica gel and water and dry it. Then TLC chamber are prepared by organic materials like acetic acid, methanol and water (3:2:1).
  6. 6. Procedure: I. Leaves are collected and dried in room temperature up to one week. II. Then leaves are crushed in mixing grinder. (Weight of leaf is 50 gm.) III. Then the leaves are extract by using socks let apparatus. IV. Four types of extraction are performed using Organic material. I) Ethanol Extraction. II) Chloroform Extraction. III) Pet. Ether Extraction. IV) Water Extraction. V. In ethanol extraction ethanol is used 250 ml. VI. In chloroform extraction chloroform is used 250 ml. VII. In pet ether extraction pet ether is used 250 ml.
  7. 7. VIII. Extraction sample are taken in to petridishs. IX. Then dried the Petridis. X. Then chake the solubility of the extract in hot and cold water. XI. Then chake the phenol coefficient by thin layer chomatography (TLC) method. XII. Than redal walkar coefficient test are performed to chake the anti microbial activity of Salmonella typhi in nutrient broth. XIII. Then chake the result.
  8. 8. Result  Observation table of solubility: Sample Cold water Hot water Ethanol extract Soluble Soluble Chloroform extract Insoluble Insoluble PET-Ether extract Insoluble Insoluble Water extract Soluble Soluble
  9. 9. Observation table of TLC: Sample Response Ethanol extract + ve Chloroform extract -Ve PET-Ether extract -Ve Water extract + ve
  10. 10. Observation table of R.W.C: Concentration Time Of contact (gm) 2(1/2) 5 7(1/2) 10 1/100 (phenol) + + - - 1/100 (ethanol) + + - - 1/200 (ethanol) + - - - 1/400 (ethanol) + - - - 1/500 (ethanol) + - - -
  11. 11. Concentration Time Of Contact (gm) 2(1/2) 5 7(1/2) 10 1/100 (phenol) + + - - 1/100 (water) + + + - 1/200 (water) + + - - 1/400 (water) + - - - 1/500 (water) + - - -
  12. 12.  Calculation: Formula: Rideal walker coefficient= (MIC of phenol/ MIC of sample) Ethanol extraction: R.W.C = (MIC of phenol/MIC of ethanol) = {(1/100)/(1/100)} = 1 Water extraction: R.W.C = (MIC of phenol/ MIC of water) = {(1/100)/(1/200)} = 2
  13. 13.  Conclusion: After performing the experiment, it was found that Rideal walker coefficient of ethanol extraction is 1 and water extraction is 2. So, 1/100 dilution of ethanol extraction is equal to 1/100 dilution of phenol and 1/200 dilution of water extraction is effective as the 1/100 dilution of phenol.

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