IMT’s Food QA/QC Assays Andy Moreno Vice President, Microbiology Sales Engineering Cell: +559-827-8245 FAX: +866-776-3746 ...
Industrial Microbial Testing (IMT) <ul><ul><li>Manufactures assays to support rapid real-time quantitative Polymerase Chai...
Traditional Culture Media vs. ~1 HOUR qRT-PCR Real-Time Data Standard Curve versus 3 to 14 days ~ One (1) Hour Slide 3 of 8
Slide 4 of 8
“ Organism Prospecting Screens” <ul><li>Water or Matrix </li></ul><ul><ul><li>Irrigation water/Ingredients/In Process/Fina...
IMT’s OneStep Assays 85  µl  molecular grade water added to primers with PCR chemistry in dried-down format 5  µl  purifie...
Typical Food QA Station & Team <ul><li>IMT TBC Assay and Cepheid SmartCycler </li></ul><ul><li>Minimally-trained QA/QC tea...
IMT Assays <ul><li>Total Bioburden Assays </li></ul><ul><ul><li>OneStep Total Bacteria Count Assay </li></ul></ul><ul><ul>...
IMT’s Assays Validate  Food Producer’s Statistical Process Control Program Periodic Testing of Randomized samples against ...
Food: Positive Release Program <ul><li>Irrigation water tested </li></ul><ul><li>Raw fruit and vegetables are washed and c...
Sample Environmental Testing* Surface Sample Collection Collect bacterial samples in filter One sample, 5 tests -Total Bac...
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Im Ts Food Qa Qc Assays 07 07 2010

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Empowering Food QA/QC teams to screen for Total Bacteria Count, Total Yeasts and Molds, E. coli, Salmonella, Listeria, in-house in about an hour.

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Im Ts Food Qa Qc Assays 07 07 2010

  1. 1. IMT’s Food QA/QC Assays Andy Moreno Vice President, Microbiology Sales Engineering Cell: +559-827-8245 FAX: +866-776-3746 [email_address] http://www.imt-pcr.com Linkedin Profile SKYPE: Andy Moreno USA Slide 1 of 8
  2. 2. Industrial Microbial Testing (IMT) <ul><ul><li>Manufactures assays to support rapid real-time quantitative Polymerase Chain Reaction microbial testing. </li></ul></ul><ul><ul><ul><li>Actionable Information: Rapid Release/99% product safe </li></ul></ul></ul><ul><ul><ul><li>Establish Positive Release Program </li></ul></ul></ul><ul><ul><ul><li>Food Safety Screening: Hold/Release time </li></ul></ul></ul><ul><ul><ul><ul><li>~One (1) hour/PCR vs. Traditional Culture Media Testing </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Ingredients, In-process Solutions, Finished Product </li></ul></ul></ul></ul><ul><ul><ul><li>Sensitivity: One (1) cell per 5 µl of sample </li></ul></ul></ul><ul><ul><ul><li>One (1) sample, many tests </li></ul></ul></ul>Slide 2 of 8
  3. 3. Traditional Culture Media vs. ~1 HOUR qRT-PCR Real-Time Data Standard Curve versus 3 to 14 days ~ One (1) Hour Slide 3 of 8
  4. 4. Slide 4 of 8
  5. 5. “ Organism Prospecting Screens” <ul><li>Water or Matrix </li></ul><ul><ul><li>Irrigation water/Ingredients/In Process/Final Product sample </li></ul></ul><ul><li>Total Bacterial Count (TBC) screen </li></ul><ul><ul><li>Is there bacteria? </li></ul></ul><ul><ul><ul><li>Presence/Absence </li></ul></ul></ul><ul><ul><ul><li>Quantitation </li></ul></ul></ul><ul><li>Total Yeasts and Molds Count (YMC) screen </li></ul><ul><ul><li>Are there yeasts and molds? </li></ul></ul><ul><ul><ul><li>Presence/Absence </li></ul></ul></ul><ul><ul><ul><li>Quantitation </li></ul></ul></ul>Slide 5 of 8
  6. 6. IMT’s OneStep Assays 85 µl molecular grade water added to primers with PCR chemistry in dried-down format 5 µl purified DNA Sample per Cepheid Tube plus 20 µ l water and primer chemistries: total 25 µ l—4 test batch ~30 minute run time Slide 6 of 8
  7. 7. Typical Food QA Station & Team <ul><li>IMT TBC Assay and Cepheid SmartCycler </li></ul><ul><li>Minimally-trained QA/QC team(s) </li></ul><ul><li>30 minute, easy-to-use, accurate DNA results </li></ul>Slide 5 of 7
  8. 8. IMT Assays <ul><li>Total Bioburden Assays </li></ul><ul><ul><li>OneStep Total Bacteria Count Assay </li></ul></ul><ul><ul><li>OneStep Total Yeasts & Molds Count Assay </li></ul></ul><ul><li>Commercialized Pathogen Assays </li></ul><ul><ul><li>E. coli spp. Assay </li></ul></ul><ul><ul><li>Salmonella spp. Assay </li></ul></ul><ul><ul><li>C. botulinum “E” Assay </li></ul></ul><ul><ul><li>Porcine Assay </li></ul></ul><ul><li>R&D Pipeline </li></ul><ul><ul><li>O157:H7 </li></ul></ul><ul><ul><li>Campylobacter Assay </li></ul></ul><ul><ul><li>Listeria mono. Assay </li></ul></ul><ul><ul><li>Enterococcus spp. Assay </li></ul></ul><ul><ul><li>Staphylococcus spp . Assay </li></ul></ul><ul><ul><li>Pseudomonas spp. Assay </li></ul></ul><ul><ul><li>Xanthomonas spp . Assay </li></ul></ul><ul><ul><li>Mycoplasma spp . Assay </li></ul></ul>Slide of 8 Samples Total Bacteria Count Pathogen of Interest Positive Release Program
  9. 9. IMT’s Assays Validate Food Producer’s Statistical Process Control Program Periodic Testing of Randomized samples against established maximum and minimum standards.
  10. 10. Food: Positive Release Program <ul><li>Irrigation water tested </li></ul><ul><li>Raw fruit and vegetables are washed and cleaned </li></ul><ul><li>Peracetic Acid and Chlorine wash bath tested at the end of each run </li></ul><ul><li>Finished product is tested </li></ul><ul><li>Environmental samples collected in plant “hot spots” </li></ul>Slide of 8
  11. 11. Sample Environmental Testing* Surface Sample Collection Collect bacterial samples in filter One sample, 5 tests -Total Bacterial Count - E. coli spp. -Salmonella spp. -Listeria spp. Collection and preparation of samples = 30 minutes Quantitative real-time PCR results in 30 minutes *Quantitatively track the presence pathogens in the environment and correlate with spoilage using statistical process control methodologies.

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