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mbbs ims msu

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mbbs ims msu

  1. 1. 1<br />WBC Count<br />Specific Equipment<br />1. White pipette<br />2. Rubber pipe<br />3. Plastic syringe<br />4. Pipette vibrator<br />5. Hemacytometer & cover slip<br /> (Counting chamber)<br />6. Microscope<br />7. Counter (hand tally)<br />8. Report form<br />
  2. 2. WBC Count<br />Reagent<br />WBC diluting fluids<br />1. Turk’s solution<br /> Glacial acetic acid<br /> Gential violet<br /> Distilled water<br />2. Two % acetic acid<br />3. One % hydrochloric acid<br />
  3. 3. 3<br />WBC Count<br />Specimens<br />1. Fresh blood<br />2. Anticoagulated blood<br /> EDTA<br /> Double Oxalate<br /> Citrate<br /> Heparin<br /> Etc.<br />
  4. 4. WBC Count<br />Procedure<br />1. Mix the blood sample thoroughly.<br />2. Fill blood into white pipette at 0.5 mark.<br />3. Fill reagent add up into the pipette <br /> to 11 mark.<br />4. Shake the pipette on the vibrator for 1 min.<br />5. Discarded the first 3-4 drops.<br />6. Fill in the hemacytometer nicely.<br />7. Allow WBC to set down for 2-3 min.<br />8. Count 4 white squares under microscope <br />9. Calculate the WBC concentration.<br />
  5. 5. 5<br />WBC Count<br />1 mm.<br />W<br />W<br />1 mm.<br />R<br />R<br />1 mm.<br />R<br />R<br />R<br />W<br />W<br />High 0.1 mm.<br />3 mm.<br />
  6. 6. 6<br />WBC Count<br />W<br />1 mm.<br />High 0.1 mm.<br />1mm.<br />
  7. 7. 7<br />WBC Count Calculation<br />1. Volume of 1 white square = 1 x 1 x 0.1 cu.mm.<br /> = 0.1 cu.mm.<br />2. Volume of 4 white square = 0.1 x 4 cu.mm.<br /> = 0.4 cu.mm.<br />3. In 0.4 cu.mm. the WBC count = N (counted No.)<br />4. In 1 cu.mm. the WBC count = N x 1 / 0.4<br /> = N x 2.5<br />5. The dilution for WBC = 0.5 / 10<br /> = 20<br />6. The final WBC count = N x 2.5 x 20<br /> = 50 N (/cu.mm.)<br />
  8. 8. 8<br />WBC Count<br />Calculation<br />White cell count = number of cells counted (N) <br /> x volume factor (=2.5)<br /> x dilution factor (=20)<br /> = N x 50<br />Normal range = 5,000 - 10,000 / cu.mm.<br /> 5 - 10 x 103 / cu.mm.<br />

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