1.
CD Based Questions
Experiment No. 1 to 10
Prof. Amol Deore
Department of Pharmacology
MVP Samaj’s Institute of Pharmaceutical Sciences, Adgaon, Nashik

2.
Identify this animal and mention its uses in experimental
pharmacology.
Answer: New Zealand Rabbit
• Used in Pyrogen testing of parenterals
• Used in Anti-diabetic studies
• Used in Anti-asthmatic studies

3.
Identify this animal and mention its uses in experimental
pharmacology
Answer: Wister rat
• Used in toxicity studies
• Used in anti-psychotic studies
• Used in analgesic studies

4.
Identify this animal and mention its uses in experimental
pharmacology
Answer: Guniea Pig
• Used in study of local anesthetics
• Used in Anti-TB studies
• Used in Anti-asthmatic studies

5.
Identify route of drug administration in mice as shown
in figures.
Answer
• Intraperitoneal route of injection in peritoneal cavity

6.
Identify route of drug administration in mice as shown
in figure.
Answer
• Oral route of administration

7.
Identify route of drug administration in mice as shown
in figures.
Answer
• Intravenous route of injection in ventral tail vein

8.
Identify route of drug administration in mice as shown
in figures.
Answer
• Subcutaneous route of injection

9.
• Which research or study is done within an entire living organism?
Answer
In vivo is Latin for “within the living.”
It refers to tests, experiments, and procedures that researchers perform in or on a whole living
organism, such as a person, laboratory animal, or plant.

10.
• Which research or study is done outside of an living organism?
Answer
In vitro (“in glass”) It describes medical procedures, tests, and experiments that
researchers perform outside of a living organism

11.
• What is meant by in silico drug design?
Answer
In silico drug design is a term that means 'computer-aided molecular design', or in
other words the rational design or discovery of drugs using a wide variety of
computational methods.

12.
Identify the blood withdrawal technique in mice as
shown in figures.
Answer
• Blood sample collection by retro-orbial plexus
in mice

13.
Identify the blood withdrawal technique as shown in
figures.
Answer
• Blood sample collection by ventral tail vein in rats

14.
Identify and mention the uses of the instrument as shown in
figure.
Answer: Eddys hot plate Analgesiometer
• The hot plate test is used in testing the effect of analgesics by
observing the reaction to pain caused by heat.

15.
Identify and mention the uses of the instrument as shown in
figure.
Answer: Electroconvulsiometer
• Electroconvulsiometer was used to induce electroshock seizures in all
experimental animals. Electrodes were applied to both the ear pinna of
animals

16.
Identify and mention the uses of the instrument as shown in
figure.
Answer: Actophotometer
• Actophotometer is used to measure locomotor activity of animals.

17.
Identify and mention the uses of the instrument as shown in
figure.
Answer: Elevated plus maze apparatus
• The elevated plus maze test is one of the most widely used tests
for measuring anxiety-like behavior.

18.
Identify and mention the uses of the instrument as shown in
figure.
Answer: Rotarod apparatus
• Rotarod apparatus is widely used to evaluate drug effects on motor
coordination, balance and motor learning in rodents. The principle of this test
is that rats or mice are first trained to walk on a rod rotating at a certain speed

19.
Identify and mention the uses of the instrument as shown in
figure.
Answer: Cook's pole climbing apparatus
• Cook's pole climbing apparatus was used to determine the
antipsychotic activity.

20.
Identify and mention the uses of the instrument as shown in figure.
Answer: Rabbit holder
• Rabbit holders are used for transporting and
restraining rabbits during experimental protocols.

21.
Identify and mention the uses of the instrument as shown in figure.
Answer: Student organ bath assemly
• This equipment is commonly used to study the physiology and
pharmacology of in-vitro tissue preparations.

22.
Label 1 to 5 as mentioned in this figure.
Answer
• 1- Dendrites, 2- Trigger Zone, 3- Axone,
• 4- Cell Body, 5- Axone terminals

23.
Label the no. 1 to 5 in eye diagram.
23
Answer
• 1- Cornea,
• 2- Pupil,
• 3- Lens,
• 4- Iris,
• 5- Retina.

24.
Give conclusion from observation table of effect of xylocaine.
24
Answer
• Loss of corneal reflex from the test eye indicate loss of corneal sensation,
therefore such drug xylocaine, procaine are used as local anesthetics.

25.
Describe the state. Give two drugs showing similar action.
25
Answer
• Because of midriasis an individual can see objects which are far away but fails to
observe objects which are nearer. The inability is called as cycloplegia.
• Mydriatics drugs: Atropine, Homatropine, Cyclopentolate

26.
Describe the state. Give two drugs showing similar action.
26
Answer
• Because of miosis an individual can see the objects which are nearer but fails to
observe objects which are far away. This inability produced is called “paralysis
of accommodation” or “accommodation of spasm”
• Miotics drugs: Physostigmine, Pilocarpine,

27.
Give conclusion from the observation table.
27
Answer
• Cyclopentolate is an anticholinergics shows Mydriasis (increases pupil size) and produces
photophobia.

28.
Give conclusion from the observation table.
Answer
• Pilocarpine is parasypathomimetics shows miosis and dose not affect the corneal and
light reflexes.