Below is a diagram showing the digestion of the lmd24 plasmid cut with the restriction enzymes
BamH1 and EcoRI. The digestions are carried out with each enzyme individually, then the two
enzymes combined.
a- What is the total size of the plasmid?
b- How many restriction sites does each enzyme have?
C- Using the diagram above, reconstruct the plasmid. begin{tabular}{|l|l|l|l|} hline & EcoRI &
BamH1 & EcoRI + BamH1 hline 20Kb & & & 18Kb & & - & 12Kb & & & 8Kb & &
6Kb & & - 4Kb & & - 2Kb & & 1Kb & & & hline end{tabular}