Mangrove Development Proposal Presentation


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  • Salt Marsh environment and dominants that biome because other plants cannot grow competively It out competes other plants because of morphological and physiological adaptations enable it to grow in waters with high salt concentrations.
  • Mangrove Development Proposal Presentation

    1. 1. Mr. Michael Gómez Ms. Ana Laura Velázquez Dr. Robert Ross UPR at Cayey RISE Program
    2. 2. Introduction <ul><li>Mangrove environment </li></ul><ul><li>Water with high concentrations of dissolved salts </li></ul><ul><li>Morphological adaptations to Salt </li></ul><ul><li>Salt secretion </li></ul><ul><li>Sequester Salt </li></ul><ul><li>Benefits </li></ul><ul><li>Secrete excess salts </li></ul><ul><li>Prevent water stress </li></ul><ul><li>Purpose </li></ul><ul><li> Observe structure of </li></ul><ul><li>salt glands </li></ul><ul><li> Observe maturation </li></ul><ul><li>of leaves </li></ul>
    3. 3. Hypothesis <ul><li>Young mangrove salt glands morphology is more capable of salt secretion than old mangrove salt glands. </li></ul>Objectives Compare the morphology of young and old mangrove salt glands.
    4. 4. Goals <ul><li>Short term goals </li></ul><ul><li>Describe histological features </li></ul><ul><li>of young and mature </li></ul><ul><li>mangrove leaves & their salt glands </li></ul><ul><li>Long term goals </li></ul><ul><li>Compare the normal growth observed in Salinas </li></ul><ul><li>to a polluted area exposed to </li></ul><ul><li>different chemicals. </li></ul>
    5. 5. Procedures <ul><li>Obtaining samples </li></ul><ul><li>Microscopy techniques </li></ul><ul><li>Paraffin Embedding </li></ul><ul><li>Sectioning </li></ul><ul><li>Mounting </li></ul><ul><li>Staining </li></ul>
    6. 6. Obtaining samples <ul><li>Salinas, PR </li></ul><ul><li>Salina’s Bay </li></ul><ul><li>White mangroves </li></ul><ul><li>Young and mature leaves (with glands) </li></ul>
    7. 7. Obtaining samples (cont.) Petioles with salt glands
    8. 8. Obtaining samples (cont.) Leaves with salt glands
    9. 9. Microscopy techniques <ul><li>Disecting scope images </li></ul>
    10. 10. Microscopy techniques (cont.) Epidermal surface (reflected vs. transmitted light) Reflected light Transmitted light
    11. 11. Microscopy techniques (cont.) Upper epidermis (reflected vs. transmitted light) Reflected light Transmitted light
    12. 12. Microscopy techniques (cont.) Upper epidermis Lower epidermis
    13. 13. Microscopy techniques (cont.) Stomata 200x darkfield
    14. 14. <ul><li>Fixation </li></ul><ul><li>Dehydration </li></ul><ul><li>Embedding </li></ul><ul><li>Blocking </li></ul><ul><li>Sectioning </li></ul><ul><li>Mounting </li></ul><ul><li>Staining </li></ul>Histological Techniques
    15. 15. Results <ul><li>Young mangrove salt gland </li></ul>
    16. 16. Results (cont.) <ul><li>Young mangrove leaf </li></ul>
    17. 17. Results (cont.) Young mangrove leaf (cross section)
    18. 18. Results (cont.) Young mangrove leaf (cross section)
    19. 19. Results (cont.) <ul><li>Mature mangrove salt gland </li></ul>
    20. 20. Results (cont.) Mature mangrove leaf (cross section)
    21. 21. Results (cont.) Mature mangrove leaf (cross section)
    22. 22. Conclusion <ul><li>Mangrove salt glands in petioles and leaves are bigger in size in young tissue than in mature tissue. </li></ul>Glands in young leaves Glands in mature leaves
    23. 23. References <ul><li>Chanita, P., et. al. Salt uptake and shoot water relations in mangroves. Aquatic Botany 78 (2004) 349-360 </li></ul><ul><li>Mangroves. 08 Jan. 2008. Marietta College Biology Department. 23 Mar. 2009 http://www/ </li></ul><ul><li>Ruzin, et. al. 1999. Plant Microtechniques and Microscopy. University of Oxford. </li></ul><ul><li>Shua, S., et. al. Molecular phylogenetic analysis of mangroves: independent evolutionary origins of vivipary and salt secretion. Molecular Phylogenetics and Evolution 34 (2005) 193-205. </li></ul><ul><li>Zouhaier, B., et. al. Contribution of NaCl excretion to salt resistance of Aeluropus littoralis (Wild) Parl Molecular. Journal of Plant Physiology 164 (2007) 842-850. </li></ul>
    24. 24. Acknowledgments <ul><li>Dr. Robert Ross </li></ul><ul><li>Dr. Eneida Díaz </li></ul><ul><li>Yadira Ortiz </li></ul><ul><li>RISE Program </li></ul>