Pres jmsb

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Pres jmsb

  1. 1. PhD course: Plant-microbe interactions - exploring microbes to improve crop productivity. (3 credits) at Makerere University, Uganda 2014 Makerere Univ: Thomas Odong, Patrick Rubaihayo SLU: Johan Meijer, Sarosh Bejai, Adnan Niazi
  2. 2. Subjects: Plant-microbe interactions or how can beneficial microorganisms compatible with crop plants be identified and further developed to support sustainable crop production by improving stress management & avoiding agrochemicals. Pathogen Beneficial microbe (PGPB/PGPR) HR-resistant plant Priming (latent defense) Growth promotion (hypersensitive reaction) SA stress abiotic-biotic (salicylic acid) JA (jasmonic acid) SAR – protection (PR-proteins) ISR - protection (?????????) Nature Chemical Biology 5, 308 - 316 (2009)
  3. 3. Target group: • Students with an interest in (sustainable) crop production. • Suitable background agronomy, life sciences,... • Cross-over microbiology, plant biology, soil science, plant pathology. • Some prior lab experience wih steril technique is advantageous. Learning goals: • To know the basic concepts of plant-microbe interactions with emphasis on beneficial interactions and how microbes can support crop production. • A survey of state-of-the-art of tools and concepts are presented. • To develop strategies on how to develop and implement these techniques into the participants specific projects and problems.
  4. 4. Teaching: Read 9 papers before course with some questions provided -survey of the topic (1 poor quality) Seminars (3 papers) - survey of the topic, quality discussion, exp design, knowledge gaps Lectures - traditional - questions at coffee brakes Practical x2 – wetlab + bioinformatics (computer/data bases) low cost: simple techniques, easy access data bases Field trip study visit – farming scale - experimental Group discussions – related to papers and generic potential/problems with biocontrol (SWAT) Exam-project presentation - Own pet subject + generic problem Follow-up - SKYPE disucssion 1/3 months post course
  5. 5. Date Sun 1 Mon 2 Tue 3 Wed 4 Thu 5 Fri 6 Sat 7Sun 8 Mon 9 Tue 10 Wed 11 Thu Read 9 papers from the literature list. Arrival - get together in the evening 9-9.30 Introduction. Course outline. JM, PR, SB, TO 9.30-12 Student presentations (10-10.30 Break) 12-13 Lunch 13.00-14.30 Plants and biotic stress. 14.30-15 Break 15.00-16.30 Plants and abiotic stress. 8.30-10 Molecular Plant-Microbe Interactions. 10-10.30 Break 10.30-12 Plant Growth Promoting Bacteria. 12-13 Lunch 13-14.30 Biocontrol agents. 14.30-15 Break 15-17 Paper discussion I. JM, PR, SB, TO 8.30-12 Practical. SB,JM 12-13 Lunch 13-14 Gene expression - techniques . XX 14-17 Practical. SB,JM 8.30-12 Practical. SB,JM 12-13 Lunch 13-14 Cell signaling, damage & death - techniques. XX 14-17 Practical. SB,JM 8.30-18 Excursion – field trip 9.00-16 Practical + Bioinformatics exercise I. AN,SB,JM, 13-15 Paper discussion 2. JM, PR, SB, TO Not scheduled 8.30-10 Functional genomics. 10-10.30 Break 10.30-12 Bioinformatics. 12-13 Lunch 13-17 Bioinformatics exercise II. AN 8.30-11 Group exercise I. JM, PR, SB, TO 11-12 Group exercise I summary. JM, PR, SB, TO 13-15.30 Group exercise II. JM, PR, SB, TO 15.30-16.30 Group exercise II summary. JM, PR, SB, TO 8.30-10.30 Paper discussion 3. JM, PR, SB, TO 10.30-11 Break 11-12 Lab practical survey. JM, PR, SB, TO 12-13 Lunch 13.30-17 Prepare presentation. 18 Dinner 9 -12 Student presentations. JM, PR, SB, TO 12-13 Lunch 13-16 Student presentations. JM, PR, SB, TO Course summary, course evaluation. JM, PR, SB, TO Follow-up meetings: 1 + 3 months later
  6. 6. Remaining ? Harmonize learning objectives? Teaching resources? Computer connection (bioinformatics)? When can the course be given? Security issues? Health/disease issues? Interaction (mix people)? Follow-up - pressure or inspiration?

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