Dna Profiling Using Pcr Notes

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DNA profiling using PCR student notes

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Dna Profiling Using Pcr Notes

  1. 1. DNA Profiling using PCR Uses these properties of DNA: DNA contains exons and introns (non-coding areas) Non-coding DNA has repetitive sequences Some are short (2 – 6 base pairs ) microsatellites or STRs that can repeat up to 100 times Different people have different length STRs Some STRs are more variable (polymorphic) between people than others Polymorphisms are shared by 5 – 20% of individuals but targeting multiple known STRs gives unique combinations. The more loci, the more discrimination. NZ targets 10 STR sites (the most variable possible) STRs are allelic – you may inherit different ones from each parent (can be homozygous and heterozugous) Homozygous STRs will not separate out with gel electrophoresis because they will be the same length; heterozygous STRs will make separate bands.

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