F A R Z A N A

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F A R Z A N A

  1. 1. Bacterial degradation of textile dyes under saline condition Presented by: Farzana Kausar
  2. 2. Introduction <ul><li>Dyes </li></ul><ul><li>A dye is a colored substance that has an affinity to the substrate to which it is being applied. </li></ul><ul><li>Amongst the synthetic dyes, azo dyes are the largest group. </li></ul><ul><li>Uses </li></ul><ul><li>These dyes are widely used in textile, food, cosmetics, pharmaceuticals and paper industry. </li></ul>
  3. 3. Problems cussed by dyes <ul><li>Chemical nature </li></ul><ul><li>Pollution </li></ul><ul><li>Disturb aquatic life </li></ul><ul><li>Carcinogenic, mutagenic </li></ul>
  4. 4. Biodegradation of textile dyes <ul><li>Microorganisms </li></ul><ul><li>Enzymes system </li></ul><ul><li>Steps involved in degradation </li></ul><ul><li>Advantages </li></ul>
  5. 5. Effects of salt on microbial degradtion <ul><li>Various salts are used in dyeing industry </li></ul><ul><li>Salt concentration up to 15-20% </li></ul><ul><li>High salt concentarion cause plasmolysis </li></ul><ul><li>Halotolerant micoorganisms </li></ul>
  6. 6. Objective of the study <ul><li>This study was planned with the following objectives: </li></ul><ul><li>Isolation of salt tolerating bacteria from dye contaminated wastewater, sludge and soil. </li></ul><ul><li>Evaluate performance of bacteria for the removal of different azo dyes under saline conditions. </li></ul>
  7. 7. MATERIALS AND METHODS <ul><li>Dyes </li></ul><ul><li>Reactive Black-5, </li></ul><ul><li>Reactive Blue BRS and </li></ul><ul><li>Reactive Golden Ovifix </li></ul><ul><li>Culture Medium </li></ul><ul><li>MSM </li></ul><ul><li>pH-7.2 </li></ul>
  8. 8. ISOLATION OF AZO DYE DECOLORIZING BACTERIA <ul><li>Sampling </li></ul><ul><li>Karachi </li></ul><ul><li>Faisalabad </li></ul><ul><li>Khewra </li></ul><ul><li>2. Isolation Procedure </li></ul><ul><li>3. Purification of Bacterial Culture </li></ul>
  9. 9. Table 1: Isolation of salt tolerant bacterial strains S-1 S-2 S-3 S-4 S-5 S-6 S-7 S-8 S-9 S-10 T-1 T-19 T-2 T-20 T-3 T-21 T-4 T-22 T-5 T-23 T-6 T-24 T-7 T-8 T-9 T-10 T-11 T-12 T-13 T-14 T-15 T-16 T-17 T-18 F-1 F-19 F-2 F-20 F-3 F-4 F-5 F-6 F-7 F-8 F-9 F-10 F-11 F-12 F-13 F-14 F-15 F-16 F-17 F-18 KS-1 KS1-19 KS-2 KS-20 KS-3 KS-21 KS-4 KS-22 KS-5 KS-23 KS-6 KS-24 KS-7 KS-25 KS-8 KS-26 KS-9 KS-27 KS-10 KS-28 KS-11 KS-29 KS-12 KS30 KS-13 KS-14 KS-15 KS-16 KS-17 KS-18 KW-1 KW-2 KW-3 KW-4 KW-5 KW-6 KW-7 KW-8 KW-9 KW-10 KW-11 KW-12 KW-13 KW-14 KW-15 KW-16 KW-17 KW-18 Saline soil of khewra salt mines Qazaffi textile mill Noshad textile mill Ocean sludge Ocean water Khewra Faisalabad Karachi
  10. 10. SCREENING EFFICIENT BACTERIAL STRAINS FOR DECOLORIZATION OF AZO DYES <ul><li>On the basis of better decolorization efficiency initially 16 isolates were selected after 24 hours of decolorization results. </li></ul><ul><li>Three most efficient strains were used for further experiment. </li></ul>
  11. 11. DECOLORIZATION OF AZO DYES UNDER SALINE CONDITIONS <ul><li>KS-26, KS-23 and T-14 </li></ul><ul><li>salinity levels ranging from 0-100 g l -1 of NaCl. </li></ul><ul><li>The medium to inoculum ratio was 20:1. </li></ul><ul><li>Decolorization of azo dyes </li></ul>
  12. 12. RESULTS <ul><li>Isolation of salt tolerated bacterial strains </li></ul><ul><li>The selected strains of bacteria capable of degrading azo dye at 50 g NaCl l -1 </li></ul>
  13. 13. Table 2: The selected strains of bacteria capable of degrading azo dye at 50 g NaCl l - 80 S-4 70 F-9 90 T-17 100 T-14 100 KS-26 100 KS-23 % Decolorization Bacterial Strains
  14. 14. DEGRADATION OF REACTIVE BLACK-5 All the three strains of bacteria were able to decolorize Reactive Black-5 up to salt concentration of 60 g NaCl l -1 . The degradation rate was affected with increase in salt concentration
  15. 15. Fig. 1: Potential of bacterial strain KS-23 for decolorization of Reactive Black-5 at different salt concentration (0-60 g NaCl l -1 )
  16. 16. Fig. 2: Potential of bacterial strain KS-26 for decolorization of Reactive Black-5 at different salt concentration (0-60 g NaCl l -1 )
  17. 17. Fig. 3: Potential of bacterial strain T-14 for decolorization of Reactive Black-5 at different salt concentration (0-60 g NaCl l -1 )
  18. 18. DEGRADATION OF REACTIVE BLUE BRS All the selected strains were able to degrade Reactive Blue BRS efficiently. Bacterial strains were able to tolerate salt concentration up to 100 g NaCl l -1 .
  19. 19. Fig. 4: Potential of bacterial strain KS-23 for decolorization Reactive Blue BRS at different salt concentration (0-100 g NaCl l -1 )
  20. 20. Fig. 5: Potential of bacterial strain KS-26 for decolorization Reactive Blue BRS at different salt concentration (0-100 g NaCl l -1 )
  21. 21. Fig. 6: Potential of bacterial strain T-14 for decolorization Reactive Blue BRS at different salt concentration (0-100 g NaCl l -1 )
  22. 22. DEGRADATION OF REACTIVE GOLDEN OVIFIX Bacterial strains were able to decolorize Reactive Golden Ovifix. All the three strains were able to decolorize Reactive Golden Ovifix in the presence of salt concentration 0-60 g NaCl l -1 .
  23. 23. Fig. 7: Potential of bacterial strain KS-23 for decolorization Reactive Golden Ovifix at different salt concentration (0-100 g NaCl l -1 )
  24. 24. Fig. 8: Potential of bacterial strain KS-26 for decolorization Reactive Golden Ovifix at different salt concentration (0-100 g NaCl l -1 )
  25. 25. Fig. 9: Potential of bacterial strain T-14 for decolorization Reactive Golden Ovifix at different salt concentration (0-100 g NaCl l -1 )
  26. 26. CONCLUSION This research suggested that under high saline conditions microbial activity is affected but there are many bacteria that have natural ability to tolerate such saline conditions. Therefore use of such halotolerant bacteria can be useful for the degradation of azo dyes under saline conditions.

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