Tangential flow filtration

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Tangential Flow Filtration [TFF] is used for clarifying, concentrating and purifying proteins.

Filtration is a pressure driven separation process that uses a membrane to separate components in liquid solution or suspension based on size and charge difference.

Types of Membrane Filtration

1. Normal Flow Filtration - Dead End Filtration
2. Tangential Flow Filtration - Cross Flow Filtration

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Tangential flow filtration

  1. 1. Tangential Flow Filtration: Membrane Types and Process Parameters http://technologyinscience.blogspot.com
  2. 2. • Tangential Flow Filtration [TFF] is used for clarifying, concentrating and purifying proteins. • Filtration is a pressure driven separation process that uses a membrane to separate components in liquid solution or suspension based on size and charge difference.
  3. 3. Types of Membranes • 1. Normal Flow Filtration - Dead End Filtration • 2. Tangential Flow Filtration - Cross Flow Filtration
  4. 4. Types of TFF • 1. Micro Filtration • 2. Ultra Filtration Ultra Filtration is catagorized into two • Virus Filtration • High Performance Tangential Flow Filtration
  5. 5. Process Goals • Final Product Concentration • Feed Volume Reduction • Extent of Buffer Exchange • Contaminant Removal Specification
  6. 6. Primary Protein Processing Goals • High Product Yield • High Product Quality • High Product Purity • Controlled Bioburden
  7. 7. TFF Membranes • Polyethersulfone (PES) • Regenerated Cellulose
  8. 8. Types of TFF Membranes • Flat Plate • Spiral Wound • Hollow Fiber
  9. 9. Key Parameters to Optimize • Cross Flow Rate • Transmembrane Pressure • Filtrate Control • Membrane Area • Diafiltration Design
  10. 10. Factors affecting Product Yield These are the four contribution of product loss during Tangential Flow Filtration • Retention Loss • Adsorption Loss • Solubility Loss • Unrecoverable hold up volume loss
  11. 11. Factors affecting Product Quality Quality of product is compromised due to aggregation or denaturation caused by • Micro cavitation • Air-Liquid Interface • High Protein Concentration • Temperature Effects
  12. 12. Thank You • Click here to Visit Bio-Resource for more details on Tangential Flow Filtration • http://technologyinscience.blogspot.com

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