Solid state fermentation

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Solid-state fermentation (SSF) or Solid Substrate Fermentation has been defined as the fermentation process occurring in the absence or near-absence of free water.
SSF employs natural raw materials as carbon source such as cassava, barley, wheat bran, rice bran, sugarcane bagasse, cassava bagasse, various oil cakes (e.g. coconut oil cake, palm kernel cake, soybean cake, ground nut oil cake, etc), fruit pulps (e.g. apple pomace), corn cobs, saw dust, seeds (e.g. tamarind, jack fruit), coffee husk and coffee pulp, tea waste, spent brewing grains, etc

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Solid state fermentation

  1. 1. Solid State Fermentation Prepared By: Ajith Kumar M Biotech Articles Protocols and More... Visit Bio-Resource http://technologyinscience.blogspot.com
  2. 2. What is Solid State Fermentation?? •Solid-state (substrate) fermentation (SSF) has been defined as the fermentation process occurring in the absence or near-absence of free water. •SSF employs natural raw materials as carbon source such as cassava, barley, wheat bran, rice bran, sugarcane bagasse, cassava bagasse, various oil cakes (e.g. coconut oil cake, palm kernel cake, soybean cake, ground nut oil cake, etc), fruit pulps (e.g. apple pomace), corn cobs, saw dust, seeds (e.g. tamarind, jack fruit), coffee husk and coffee pulp, tea waste, spent brewing
  3. 3. Advantages of SSF •Process is simple •Cost Effective •Less Effluent release, reduces pollution •High Titers, (High Product yields) •Aeration Process is easy •Resembles the natural habitat of some fungi and bacteria •Easier downstream processing
  4. 4. Major Factors of SSF •Selection of Micro-organisms •Substrate •Process Optimization •Product Isolation & Purification
  5. 5. Selection of Micro-organism •This is one of the key factor for improved yields of the product. •Bacteria, Yeast and Filamentous Fungi can be used. •Filamentous Fungi has shown better results growing in the solid substrate
  6. 6. Substrate •Substrate also plays important role in determining the growth of micro-organisms, there by increasing the product yield. •Substrate is chosen such a way that it should provide physical support as well as nutrients to the growing culture. •Substrate is of two types: •One is Specific substrate, which requires suitable value-addition and/or disposal. •The second is for producing a specific product from a suitable substrate.
  7. 7. Process Optimization •Includes the optimization of physico-chemical and Biochemical Parameters •Size, initial moisture, pH and pre-treatment of the substrate •Relative humidity, temperature of incubation, agitation and aeration, age and size of the inoculum •Nutrient Supplementation such as N, P and trace elements, supplementation of additional carbon source and inducers •Extraction of product and its purification
  8. 8. Problems in SSF •Heat Transfer: One of the main difficulty is to control the temperature during the fermentation process. •Heat is generated during the metabolic activities of micro-organisms, since the substrate used has low thermal conductivity heat removal will be slow. •When the heat generated goes beyond certain level, which will result in product denaturation and will affect growth of microbe, ultimately ending up in reduction in yield and quality of the product.
  9. 9. Applications
  10. 10. Economic Sector Applications Examples Agro-Food Industry Traditional Food Fermentations Koji, Tcznpch, Rae, Attickc, Fermented cheeses Mushroom Production & spawn Agaricus, Pleurotus, Shn-take Bioconversion By-products Sugar pulp Bagasse Composting, Detoxication Food Additives Flavours. Dyestuffs. Agriculture Biocontrol , Bioinsecticide Beauveria Metarhizium, Tricho derma PlantGrowth Hormones / Enhancers Giberellins, Rhizobium, Trichoderma Industrial Fermentation Enzymes production Amylases, Cellulases Proteases, Pectinases, Xylanases Antibiotic production Pencillin, feed & Probiotics Organic acid Production Citric acid, Fumaric acid,etc Fungal Metabolites Alkaloids Ethanol Production Malting and Brewing
  11. 11. References •General and microbiological aspects of solid substrate fermentation •Basics of SSF •Pandey, Ashok (2008, June 13). Solid-state fermentation. SciTopics
  12. 12. Thank You... Biotech Articles Protocols and More... Visit Bio-Resource http://technologyinscience.blogspot.com

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