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Presentation berlin 2015 alfredo garcia fernandez

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Presentation berlin 2015 alfredo garcia fernandez

  1. 1. Silene ciliata (Caryophyllaceae) as a model species in a climate change scenario: our multidisciplinary approaches García-Fernández, A., Morente-López, J., Lara-Romero, C., Rubio-Teso, M.L., Iriondo, J.M. Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, E-28933 Móstoles, Madrid, Spain.
  2. 2. 10 years with Silene ciliata in 10 minutes using 10 slides García-Fernández, A., Morente-López, J., Lara-Romero, C., Rubio-Teso, M.L., Iriondo, J.M. Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, E-28933 Móstoles, Madrid, Spain.
  3. 3. • Circum-mediterranean alpine chamaephyte • Elevation range: 1500 – 2500 m • 2 important features: • Polyploid karyotypes – 2n = 24 to 2n = 192 • Phenotype variability • Description of subspecies: – Subsp. graffieri (Eastern mountains) – Subsp. ciliata (Western mountains) – Other subsp. and varieties Silene ciliata Pourret Introduction Past studies Current researches Future prospects
  4. 4. Silene ciliata Pourret Introduction Past studies Current researches Future prospects • Plastid markers support the existence of two subspecies • More haplotype diversity in western group • Vicariance processes, dispersal barriers and geographical disconnection associated to glaciation-interglaciation periods Kyrkou et al. (2015) PeerJ
  5. 5. Introduction Past studies Current researches Future prospects Silene ciliata Pourret • During the last decade, more than 10 papers published with the species. • Mediterranean mountains, hotspot of biodiversity and endemisms. • Altitudinal gradients summarize environmental variabilities.
  6. 6. Introduction Past studies Current researches Future prospects Silene ciliata Pourret • Floral asynchrony Intermediate population Low population High population Gimenez-Benavides et al. (2007) New Phyto.
  7. 7. Introduction Past studies Current researches Future prospects Silene ciliata Pourret • Floral asynchrony • Local adaptation, reciprocal transplants Intermediate population Low population High population Gimenez-Benavides et al. (2007) Ann. Bot.
  8. 8. Introduction Past studies Current researches Future prospects Silene ciliata Pourret • Floral asynchrony • Local adaptation, drought resistance Intermediate population Low population High population Fv/Fmphtosynteticstatus Days without water supply García-Fernández et al. (2012) Plant Bio. Low pop. Inter. pop. High pop.
  9. 9. Introduction Past studies Current research Future prospects Silene ciliata Pourret • Central – marginal hypothesis • Niche model prediction in Central Spain
  10. 10. GuadarramaGredosBéjar Introduction Past studies Current research Future prospects Silene ciliata Pourret • Central population • Marginal populations • Central – marginal theory • Niche model prediction in Central Spain • Populations selection, based in: – 3 mountains – Different population size
  11. 11. Introduction Past studies Current research Future prospects Silene ciliata Pourret • Central – marginal theory • Niche model prediction in Central Spain • Populations selection • Two complementary approaches:  Ex situ sowing experiment at optimum conditions in growth chamber and greenhouse environment (mother plant x type of cross x block x pop. = 8640 seeds).  In situ sowing experiment at the locations of the 6 marginal populations (mother plant x type of cross x block x pop. = 24000 seeds)
  12. 12. Introduction Past studies Current research Future prospects Silene ciliata Pourret Reference-based transcriptome assembly Silene latifolia Reference Genome T G T C G G T C T T G T C G G T C T T G T C A G T C T T G T C A G T C T SNP calling – Reads2SNP High Low Differential expression Candidate Genes High Low Candidate Genes Functional annotation & Enrichment analysis • Next Generation Sequencing. • Transcriptome analyses and SNP calling.
  13. 13. Introduction Past studies Current research Future prospects Silene ciliata Pourret Thanks for your attention Prof. Jose M. Iriondo Group leader Javier Morente-López Ph.D student Luisa Rubio Ph.D student Dr. Alfredo García-Fernández Dr. Carlos Lara-Romero
  14. 14. RUI AGI CAM SES NAJMOR F1 F2 F3 F1>F2F1<F3 F1=F2,F3 F1=F2,F3F1=F2,F3 Inbreeding depression No inbreeding depression F1<F2 Adaptation from MOR Maladaptation from RUI In situ F1<F2,F3F1<F2,F3 F1=F2,F3 F1=F2,F3 Inbreeding depression No inbreeding depression F1<F2,F3 Inbreeding depression Ex situ F1=F2,F3 He= 0.63 FIS= 0.33 Ne= 10 He= 0.75 FIS= 0.32 Ne= 25 He= 0.66 FIS= 0.19 Ne= 12 He= 0.70 FIS= -0.12 Ne= inf He= 0.70 FIS= 0.39 Ne= 66 He= 0.51 FIS= 0.31 Ne= 131 Introduction Past studies Current research Future prospects
  15. 15. Introduction Past studies Current research Future prospects Silene ciliata Pourret • Next Generation Sequencing. • Transcriptome analyses and SNP calling – 288 genes expressed differentially

Editor's Notes

  • In parallel we conducted a genetic characterization of the marginal populations using microsatellite markers


    Inbreeding depression in MOR population (García-Fernández et al., 2012a).
    Najarra: He y Fis de los cuatro primeros marcadores de Carlos (uno de ellos distinto al utilizado en el resto del estudio)
    Ne calculado para los 8 marcadores de Najarra
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