Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

1

Share

Blood plasma fractionation process

Human Plasma is the yellowish liquid present in the human blood that constitutes 55% of its volume and is responsible for all the major circulation of it throughout the human body. There are many other proteins and substances present in the human plasma in minute quantities that also can be separated through the process of Blood plasma fractionation.

Related Books

Free with a 30 day trial from Scribd

See all

Related Audiobooks

Free with a 30 day trial from Scribd

See all

Blood plasma fractionation process

  1. 1. Blood plasma fractionation processPlasma contains a large variety of proteins including albumin, immunoglobulin, and clottingproteins such as fibrinogen in it. These proteins present in it have many important therapeuticuses and clinical uses and can work wonders when used in development of various medicines.These substances are also widely used in medical researches to develop therapies and vaccinesfor chronic diseases like cancer, tumors and AIDS etc.In order to get the benefit of these live saving substances plasma is extracted from the humanblood through a process called blood fractionation. Human Plasma is the yellowish liquidpresent in the human blood that constitutes 55% of its volume and is responsible for all the majorcirculation of it throughout the human body. When this extraction is carried out we get aclear solution of plasma in the upper phase, the Buffy coat, which is a thin layer of leukocytesmixed with platelets in the middle and Erythrocytes (Red Blood Cells) at the bottom of thecentrifuge tube. After getting these components separately in the tube, plasma can be separatedeasily and stored for further use.Now once the Human Plasma is obtained, its various components can be separated for furtherusage. However it is also very beneficial and can be used in various forms to create medicationswholly. The process of separating the various components of Human Blood Plasma is called asBlood plasma fractionation.The Human Blood Plasma contains thousands of proteins, however, identifying them presentschallenge due to the wide range of concentrations present in it. Let us see different componentsand their concentration in it. Albumin constitutes about 60% of the total protein in plasma and is present at concentrations between 35 and 55 mg/mL. It is the main contributor to osmotic pressure of the blood and it functions as a carrier molecule for molecules with low water solubility such as lipid soluble hormones, enzymes, fatty acids, metal ions, and pharmaceutical compounds. Due to the structural integrity of albumin it remains stable under conditions where most other proteins denature.
  2. 2. Fibrinogen is present at concentrations 1.5-4.0 g/L in Human Blood Plasma or about 7 µM. It is the principal protein responsible for clotting in the human body. Immunoglobulins are the substances that fight against infections in human body. Our bodies create antibodies (Immunoglobulin’s) against disease causing agents when infections occur.Apart from these there are many other proteins and substances present in the human plasma inminute quantities that also can be separated through the process of Blood plasma fractionation.The first practical large scale method of blood plasma fractionation was developed by Edwin J.Cohn during World War II. This process is also known as cold ethanol fractionation as itinvolves gradually increasing the concentration of ethanol in the solution at 5oC and 3oC. TheCohn Process exploits differences in properties of the various proteins, specifically, thehigh solubility and low pl of albumin. However, at each stage certain proteins are precipitatedout of the solution and removed. The final precipitate is purified albumin.Several variations to this process also exist, including an adapted method by Nitschmann andKistler that uses fewer steps, and replaces centrifugation and bulk freezing with filtration anddiafiltration. However, the ultimate goal of processing is to get a purified component that can beused for making injection or transfusions. In addition to the clinical uses of a variety of itsproteins, it has many analytical uses too. Blood plasma fractionation obtains it in purified statethat can be utilized for many purposes and have many beneficial factors in it.
  • jabdolalizadeh

    Nov. 28, 2016

Human Plasma is the yellowish liquid present in the human blood that constitutes 55% of its volume and is responsible for all the major circulation of it throughout the human body. There are many other proteins and substances present in the human plasma in minute quantities that also can be separated through the process of Blood plasma fractionation.

Views

Total views

8,468

On Slideshare

0

From embeds

0

Number of embeds

4

Actions

Downloads

0

Shares

0

Comments

0

Likes

1

×