Biofilm destructive system


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  • Strong extracellular matrix makes highly resistant to antibiotics. The extracellular matrix prevents molecules from passing through to the bacteria, even molecules as small as hydrogen peroxide do not have a strong effect on biofilms. Biofilms also have a much slower growth rate making antibiotics more ineffective. Many biofilms also exhibit over expression of multiple drug resistant pumps which make it even harder to destory.
  • Biofilms area serious problem in health care because they can form on many types of catheters such as urinary, heart, respiratory, and intravenous just to name a few. Biofilms can also form on prosthetic valves, teeth and contact lenses. The CDC reports that biofilms are responsible for around 65% of nosocomial infections. The formation of biofilms on catheters and valves can lead to chronic infections or chronic inflammatory responses in patient. The current protocol is to just have an emergency surgery to remove and replace the infected valve or catheter which not only raises the cost of health care for the patient but can also further weaken the patient’s immune system. Formation can lead to further tissue damage in the patient
  • There are 38 million contact lens wearers in the US and 138 million contact lens wearers worldwide. Poor cleaning habits can lead to biofilm formation on contact lens cases. The rate of contamination varies from 24 to 81%. \1 in 3 users clean their contact lens case monthly even less often. Contact lens contamination can lead to a number of infections and disease such as conjunctivitis (pink eye), saphylococci, and microbial keratitis. Microbial keratitis is the most severe of these infections which is the inflammation of the cornea and is specifically related to contact lens use and improper cleaning methods. There is no universal regimen for cleaning these contact lens cases or product that prevents that cleans biofilms off of contact lens cases.
  • Contact lens case disinfection is often thought of as a consequence of contact lens disinfection because it is assumed that lens cleaning and disinfection solutions also clean and disinfect the case. Cleaning with heat is the least effective and currently not used because it can distort the contact lens Currently the existing solutions involve hydrogen peroxide solutions or multipurpose solutions that you would find at walgreens. These solutions you normally just soak and wipe them down. Regarding contact lens cases some have silver incorporated into the biomaterials to make the surface hydrophilic to dissuade biofilm formation, but obviously this is a problem for people who are allergic to silver. These solutions rely a lot on user compliance. A study showed that only 26% of people cleaned their contact lens cases daily and one in three clean their case monthly or even less. We hope to create a product for this niche.
  • Purilens is designed to clean the lends and case without the use of solution. The process only takes 15 minutes.Novelens claims to keep used solution deposits away from the fresh solution while in the case to discourage biofilm formation.Finally ,QuickPure is a battery powered device that generates ozone to clean and disinfect the lens.
  • In addition to the aforementioned solutions, there are a couple of patented design solutions. The Johnson and Johnson vision care uses UV radiation to clean the contact lens case and contact lens. The Compliance Case corporation has a “self-destructive” case. So the case will leak if there is a change in environment in the case and alert the user that the case needs to be changed. Both are potential embodiments of the design
  • Needs to cost $60 for us to make
  • To measure the efficacy of our product, we will be using the following equation where CFU stand for colony forming unit.Similar power consumption to a videogame player we estimated the product to have a power output of around 0.25kW. The 0.099 dollars/kWhr is the national average for power draw.Conversational speech is 70dB
  • I’m the consumer liason and lead ergonomics designer so I will draft the interview questions for contact lens users and lead the interviews. I will also focus on making sure that the design of the product contributes to easy and effective use by the consumer. Deborah is the materials expert and lead mechanical designer so she will focus on the physical mechanisms and design of the product. Nick is the lead electrical designer and time manager. He will focus on creating functional circuitry and ensure that the team stays on track. As a group we will all stay in contact with our client through email. We will all update the website and perform risk management after integrating all aspects of the project.
  • Biofilm destructive system

    1. 1. BIOFILM DESTRUCTIVE SYSTEM Nkele Davis Partners: Deborah Ohiani-Jegede, Nick Xydis
    2. 2. Biofilms  Created by free- floating bacteria that adhere to a surface  Form a complex and layered community with extracellular matrix  Biofilms then create more free- floating bacteria
    3. 3. Biofilm Resistance  Resistant to:  antibiotics  immune response  Typical response to biofilm growth is to dispose of the infected product
    4. 4. Biofilms and Health Care  Formation on catheters (urinary, heart, respiratory, intravenous etc.)  Prosthetic valves, artificial hips, teeth, contact lenses  CDC reports biofilms are responsible for 65% of infections
    5. 5. Biofilms and Contact Lenses  Improper cleaning methods by users can lead to biofilms forming on contact lens cases  Rates of contamination on contact lens cases vary from 24% to 81%  Contact lens contamination can lead to microbial keratitis, conjunctivitis, staphylococci
    6. 6. Project Scope  Design a product that can destroy biofilms on contact lens cases  Focus on biofilms that cause microbial keratitis  Portable, handheld device  Reasonable application time (<1hr)  Biofilm inactivation rate of at least 95%
    7. 7. Existing Solutions  Case cleaning happens as a consequence of lens cleaning  Heat  Hydrogen Peroxide solutions  Multipurpose solutions  Silver impregnated cases to dissuade biofilm formation
    8. 8. Existing Solutions Purilens  Magnetic pedal on a flexible arm is used to agitate the saline solution and loosen deposits from the lenses  UV light (253.7 nm) is used to disinfect the lenses, case, and saline solution  15 minute application period Novelens  Two chamber case that keeps lens used solution (containing deposits) separate from fresh solution during the disinfection process to discourage biofilm formation QuickPure  Battery-powered device which generates ozone as needed from the oxygen in air and bubbles it into barrel to clean and disinfect the lenses and the case  3 minute application period
    9. 9. Existing Solutions Johnson & Johnson Vision Care  A patent for a lens storage case that uses disinfecting UV radiation to clean lenses and the case The Compliance Case Co  A patent for a limited use, self destructive contact lens case  case leaks in a pre- determined fashion to alert user of end point of safe utilization
    10. 10. Design Specifications/Requirements  Usability  Within 3” x 5”  Weight: < 5 lb  Operating time: < 1 hours  Visual timer display  Set up Time: < 30 seconds  Sound during application: < 40 dB  Autonomous system, turns on and off automatically  Waterproof  Economics  Cost: < $200 (prescribed by doctor)  Power Draw: < .25kWhr per application  Effectiveness  95% biofilm destruction  biofilm growth prevention  No damage to lens case and lens  Durability  meets all other specification requirements after 5
    11. 11. Preliminary Analysis 
    12. 12. Design Schedule September  30th – Preliminary Presentation October  2nd – Preliminary Report Due  5th – Complete User Interviews  7th – Website Operational  9th – DesignSafe Report  11th – Brainstorm Application Types  13th – Brainstorm User Practices  18th – Evaluate/Improve Design Ideas  30th – Progress Presentation/Report
    13. 13. Design Schedule November  2nd – Narrow Physical Design  8th – Contact Manufacturers  10th – CAD model of Design  15th – List of Materials  16th – Design Verification  22nd – Final Paper Draft  29th – Final Paper Revision December  2nd – Final Presentation  4th – Final Report Due
    14. 14. Organization of Team  Nkele  Consumer Liaison, Lead Ergonomics Designer  Deborah  Materials Expert, Lead Mechanical Designer  Nick  Time Management, Lead Electrical Designer  Shared  Client Contact  System Integration  Informational Research  Risk Management  Website Upkeep
    15. 15. Questions??