Shojinmeat Project slides for New Harvest 2017 NYC
New Harvest 2017
11.Oct.2017, New York City
Shojinmeat Project -
Citizen Science for Cellular Agriculture
Citizen Science movement for cellular agriculture
#Culture & thoughts
The first startup
Overview - latest developments
Still self-funded and independent - not affiliated to university or corporation
Need bigger and cheaper
Need to be understood
view on clean
with Kyoto Uni.
Biohacking / DIY-bio?
Accessible, transparent, low-cost
“It’s all on the web!”
“You can do it too!” “We don’t need that high grade”
Where do we do experiments?
Leave-a-Nest teaching lab
Open Bio Club, FabCafe Tokyo
Homes and kitchens
Tissue and organ factory, TWIns lab
Sports drink culture media
Proliferation of mouse L6 in DMEM/Dakara 10% FBS
#pH and osmolality of Dakara adjusted by NaHCO3(s) and 2M NaOH
Fluid name osmolality pH
DMEM(-, hi glu) 345 7.4
Pocari 338 3.4
Aquarius 291 3.37
Amino-Value 4000 289 3.63
AminoVital Gold 186 3.33
Vitamin Water 302 3.3
Green DAKARA 322 3.28
Amiiru Water 249 3.4
Mamoru Chikara 546 3.58
Yeast extract as serum alternative?
“Yeast extract for animal cell culture”
(Sheffield Bioscience Ltd.)
Phosphate buffer & salt
Papain, 65℃, stir, ~24h
to “digest” yeast
use in cell culture
No bacterial contamination
Preparation cost: 10¢/L
Species Cell identity sYE conc. Result
Human HeLa Human cervical cancer 10％ Successful (but for how long?)
Human Human Embryonic Kidney 293T 10％ Successful (same as above)
Mouse Germ cell 10％ Unsuccessful
Mouse Ovarian somatic cell, primary 10％ Successful (but for how long?)
Mouse Hepatocyte, primary 10％ Successful (same as above)
Mouse Fetal cell, primary 10％ Successful (same as above)
Mouse Myoblast, primary 10％ Initially successful, but cell
division halts after ~3gen.
Mouse Embryonic stem cells 10％ Unsuccessful
Yeast extracts do have limits.
Some growth factors are missing.
Networked cell culture setup “Culnet System” supplies the
ingredients missing from yeast extract
Ohlsson C et al., 2009, Endocr Rev
Francis GL, 2010, Cytotechnology
“Culnet System” prototype
Japan pat. 6111510
Breaking the limits
So, where are we for 100g of meat?
Co-culture Use yeast
Use food grade
Use food grade
“fish fat beef”
“beef/pork cellular mix”
“DHA/Omega3 plus meat”
Integriculture takes care
of those business things
Shojinmeat Project and Integriculture Inc.
How to make ⇒ Open
How to scale ⇒ Proprietary
※We need legal assistance for opensourcing
“how to make”- will Creative Commons work?
Level of personalization
“Democratized Cellular Agriculture”
Citizens act and
set the direction
A direction being set - “teens can do it”
“Growing meat at home”,
Takeaways and acknowledgements
Clean meat needs to be cheap
“DIY bio” based research is
finding the way. (“Culnet System”)
Integriculture Inc. aims to
scale this technology.
Citizens set the direction and
businesses scale it.
And Everyone in Shojinmeat Project!!