Clearly state the purpose—the goal of this project was to study and analyze tea bark to characterize the chemical composition
We need to separate out the different components of the bark and compare to what is known in the literature. Experimental methods that we use to extract and analyze are compared to studies already done to identify components of the natural product.
These people who live in Phillippines often use natural products---those of us in industrial countries usually buy pharmaceutical products over the counter but they have side effects. Allegra—expensive some people get sick from generic drubs, unbuffered aspirin or ibuprofen hurts your stomach but cheaper. What is in there? What are they drinking is it more gentle than the drugs we use?
These trees have been used for medicinal studies in the literature---details of the medicinal compounds will be given in my paper----we have designed protocols from our samples based on these.
Talk about the process low solubility making tea---dark not transparent
This is like a tea bag and we are making tea----we boil 60 minutes let it steep for 18-24 hrs. we need to extract as much as possible moving forward to analyze
We concentrated the sample from 50 mL to 5 mL (10X concentrated) We crystallize to to purify (How you make aspirin) this process is based on solubility of compounds and temperature
We separated solid from liquid by vacuum filtration
We separate into different components to isolate pure compounds of the natural mixture to identfy them
This is a physical method we use to separate based on differences in boiling points
Explain the process
Identify the compounds a positive control is benzoic acid and benzamide because these are common deriviatives of ingredients in food (preservative) and medicine. Also tested the pH
Compare melting points to to literature values (known and researched compounds). Benzoic acid others
Important to society
Why is this topic relavent or significant ---for the classroom new protocols for instruction but for us (society) we need to study more natural products in genreal because they are accessible for everyone and less side effects but the challenge is how compouns are presented in the mout (amylase) is very different than how they are absorbed in the gue (ph in stoach and enzymes) and how cells will process those molecules—VERY HARD TO IDENTIFY THE “MAGIC BULLET” COMPOUND that is helpsul
Tree Bark Sample
FROM THE PHILIPPINES, AS A NATURAL PRODUCT
Honors Component for SCI 281
Prof. Kimberly Stieglitz
Dec. 15, 2014
Unknown Tree Bark Sample from Philippines
Medicinal or Analgesic Properties?
The Aeta People – An indigenous tribe of the Philippines
Pterocarpus Indicus Willd,
Trees in the Philippines
with known medicinal properties
Tea of the
Re-suspended in water)
of Sample A (Filtered Liquid)
Products of Sample A
Distillate Liquid Remaining solution
Vacuum filtered Vacuum filtered
Thin Layer Chromatography
Liquid Samples (A, C, D, and E)
Sample C (the initial solid, re-suspended in water) was the
only one to test positive for an active compound
Melting Point Apparatus
Melting Points of Solid Samples (B, F, and G)
Melting Points of 2 out of 3 solids
were determined to be above 150 °C
Further testing needed in order to
determine exact melting points
Closer to characterization based on
comparison to literature
WHOLE PRODUCTS EXTRACTION
• Shriner, Ralph L., Christine K. F. Hermann, Terence C. Moril, David Y.
Curtin, and Reynold C. Fuson. The Systemic Identification of Organic
Compounds. 8th ed. Hoboken, NJ: Johnson Wiley & Sons, 2004. Print.
• Seager, Spencer L., and Michael R. Slabaugh. Chemistry for Today:
General, Organic, and BioChemistry. 8th ed. Belmont, CA: Mary Finch,
/Cole, Cengage Learning, 2011. Print.
• Seager, Spencer L., and Michael R. Slabaugh. Safety-Scale Laboratory
Experiments for Chemistry for Today. 8th ed. Belmont, CA: Mary Finch,
/Cole, Cengage Learning, 2011. Print.
• Sasidharan, S., Y. Chen, and L. Yoga Latha. "Extraction, Isolation and
Characterization of Bioactive Compounds from Plants' Extracts." African
Journal of Traditional, Complementary, and Alternative Medicines (2010).
NCBI. African Networks on Ethnomedicines. Web. 15 Nov. 2014.
• S, Wang, Ding S, Zhang X, and Zhang L. "A New Medicinal Plant
Pterocarpus Indicus." NCBI (1997). NCBI. Web. 15 Nov. 2014.
• "A Bioactive Polyphenolic Constituent in the Bark of Pterocarpus Indicus,
Willd. I. Isolation and Characterization.” CiNii. Web.
• Mbikay, Majamba. "Therapeutic Potential of Moringa Oleifera Leaves in
Chronic Hyperglycemia and Dyslipidemia: A Review." Frontiers in
Pharmacology (2012). Web. 25 Nov. 2014.
• Fahey, Jed W. "Moringa Oleifera: A Review of the Medical Evidence for
Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1." Tree For
Life Journal (2005). Trees for Life Journal. Web. 20 Nov. 2014.