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THC in Hair Presentation.

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THC in Hair Presentation.

  1. 1. THCA in Head Hair: A Comparison with Fingernail
  2. 2. Conflict of Interest Employees of USDTL • Privately held company • Commercial laboratory • Sells hair testing services • Internally funded • No role in: • Study design • Data collection • Analysis • publication
  3. 3. OBJECTIVES Marijuana usage trends THCA in hair and nail Validation w 10 fg/mg LOD Comparison hair and nail Conclusions
  4. 4. Trends of Marijuana Use Most common illicit drug 2010-17.4 M past month users 2009 factor 376k ED visits
  5. 5. Trends of Marijuana Use 23 States and DC Medical use Decriminalized Both Legalized
  6. 6. Trends of Marijuana Use Daily use 12th graders 6.5% from 5.1% in 2007 12th graders negative perception of regular use 44.1%, lowest since 1979
  7. 7. What Do We Look For? THC THCA
  8. 8. Detection Windows
  9. 9. HOW DO DRUGS GET INTO THE HAIR? #1 ENVIRONMENTAL EXPOSURE SMOKE FUMES TRANSFERANCE CONTACT
  10. 10. HOW DO DRUGS GET INTO THE HAIR? #2 SWEAT AND OIL THESE DRUG AND METABOLITE CONTAINING FLUIDS BATHE THE HAIR SHAFT
  11. 11. HOW DO DRUGS GET INTO THE HAIR? #3 THE BLOOD
  12. 12. Specimen Type: Why Fingernail?
  13. 13. Anatomy of Fingernail NAIL MATRIX NAIL PLATE NAIL BED
  14. 14. Fingernails Are Made of Keratin o Keratin: fibrous structural protein of hair, nails, horn, hoofs, wool, feathers, and of the epithelial cells in the outermost layers of the skin. The polypeptide chains of keratin are arranged in parallel sheets held together by hydrogen bonding. ~Encyclopedia Britannica
  15. 15. Fingernails Are Made of Keratin o These keratin sheets stack on top of each other, giving the nail thickness and length, making adulteration of the specimen difficult.
  16. 16. Incorporation of Drug Biomarker: a measurable substance whose presence is indicative of ingestion or exposure to substances of abuse (SOA).
  17. 17. Biomarker Absorption o Biomarkers can be found in nails as early as 1-2 weeks after substances of abuse are ingested. o The time period during which drug ingestion can be detected depends on the substance used, the amount used, and personal metabolism.
  18. 18. THCA Validation Specs Single point Calibrator – 50 fg/mg LOQ – 20 fg/mg LOD – 10 fg/mg ULOL – 1000 fg/mg Controls 20, 62.5, and 400 fg/mg Robust – minimum 22 consecutive days
  19. 19. THCA Validation Results LOD 10 fg/mg LOQ 20 fg/mg ULOL 500 fg/mg Imprecision < 13.5% Bias < 13.8% Stable
  20. 20. What is a Femtogram 1000 fg = 1 pg 1000 pg = 1 ng
  21. 21. THCA Low Control
  22. 22. The Collection of Specimen NIAAA – 1 R44 AA016463-02 (Phase II) UW- Milwaukee- CABHR De-identified Remnants
  23. 23. POSITIVITY RATE THCA HAIR/NAIL
  24. 24. MEAN CONCENTRATIONS THCA HAIR/NAIL Paired Sample t-Test Difference 1449.2 +/- 745.3 t = 1.944, df = 59, P < 0.05
  25. 25. 60 Matched Pairs of Hair and Nail 14000 12000 10000 8000 6000 4000 2000 0 r = 0.974 0 500 1000 1500 2000 2500 Nail THCA (fg/mg) Hair THCA (fg/mg)
  26. 26. LIMITATIONS Accurate self-report Verified dose administration Demographics Prevalence not generalizable Personal hygiene Cosmetic treatment
  27. 27. Published Results
  28. 28. CONCLUSION Method is very sensitive & robust Nail is a suitable alternative [THCA] hair/nail strongly associated Slightly higher positivity rate in nail [THCA] up to 5x higher
  29. 29. Thank You All Rights Reserved. Copyright © 2013 United States Drug Testing Laboratories, Inc.

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