Biotechnology

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Biotechnology

  1. 1. A wide ranging scientificfield which includes themanipulation of livingorganisms that results innew products orprocesses by that cell.
  2. 2. Gregor Johann MendelDiscovered the LawsGoverning the GeneticInheritance of Traits byScientific Experimentation.Founded Modern Genetics.
  3. 3. *Creation of RECOMBINANTDNA*GENE CLONING*GENE TRANSFER
  4. 4.  When DNA is extracted from an organism, all its genes are obtained In gene (DNA) cloning a particular gene is copied (cloned)
  5. 5. WHAT IS GENE CLONING ?• To "clone a gene" is to make many copies of it• Act of making many identical copies of gene• Gene can be an exact copy of a natural gene• Gene can be an altered version of a natural
  6. 6. Cell based DNA cloning Cell-free DNA cloning (PCR)
  7. 7. Blood sample DNA is extracted- here from blood DNA Restriction enzymes, e.g. EcoRI, HindIII, etc., cut the DNA into small pieces Different DNA pieces cut with the same enzyme can join, or recombine. RESTRICTION ENDONUCLEASE
  8. 8. PCR technique ds DNAStep 1 DenatureStep 2 AnnealStep 3 Extend
  9. 9. 1st cycle 2nd cycle 3rd cycle 4th cycle 5th cycle 6th cycle
  10. 10. * Flavr Savr Tomato*Golden Rice *BT Cotton * BT Brinjal
  11. 11. DELAYED RIPENING IN TOMATO
  12. 12. VIRUS RESISTANT TOMATO
  13. 13. NORMAL GOLDENRICE RICE
  14. 14. BIOTECHNOLOGICALAPPLICATIONS IN MEDICINE ProteinPharmaceuticals Vaccines & Therapeutic Agents Diagnostics: Protein or DNA Based Gene Therapy Stem Cell Research Cancer Treatment
  15. 15.  Insertion of a new “healthy” gene into the organism to provide needed (usually) proteins, hormones etc. Gene is carried into the host by a viral vector that has been disabled Can provide relief for many genetic diseases Problems: Jessie Gelsinger, xscid babies immune responses, and cancer
  16. 16. ORAL VACCINEHUMULIN
  17. 17. 1983 Transgenic Mice 1997 Cloned sheep
  18. 18. THANKSSS !!!

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