Generation of 2D PAGE Experimental Data:
The Horse gram leaf proteome study is employed to examine the differential proteins expressed in leaf during drought stress and irrigated condition. The proteins are separated using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometric analysis of differential proteins spots. In that 10 protein spots were differentially expressed in leaf tissues of varied molecular weight ranging between 17-54 kD and isoelectric point covering the range of 4-7. The protein spots are analyzed using matrix-assisted laser desorption ionization time of-flight mass spectroscopy (MALDI-TOF-MS).
This database runs on WAMP server with HTML as the front end and MySQL 5.5.20. as the backend using PHP 5.3.10(Hypertext Preprocessor) as interface. The results are displayed in such a manner that the proteins that were expressed in the irrigated and unirrigated leaves are compared and their results are displayed. It also includes calculated properties of proteins such as molecular weight, pI value, expression sequences obtained using MALDI-TOF and accession id. Then homology search is implemented to determine the most identical protein using protein database uniprot.The link which is provided for the accession id will connect directly to NCBI.The spot information includes homologous protein name, sequences coverage, mascot score(%),mascot result, sequence coverage. Theoretical molecular weight and pI.