TNAU PDB- Tamil Nadu Agricultural University proteome database- Horse gram proteome

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Generation of 2D PAGE Experimental Data:
The Horse gram leaf proteome study is employed to examine the differential proteins expressed in leaf during drought stress and irrigated condition. The proteins are separated using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometric analysis of differential proteins spots. In that 10 protein spots were differentially expressed in leaf tissues of varied molecular weight ranging between 17-54 kD and isoelectric point covering the range of 4-7. The protein spots are analyzed using matrix-assisted laser desorption ionization time of-flight mass spectroscopy (MALDI-TOF-MS).
Database Schema:
This database runs on WAMP server with HTML as the front end and MySQL 5.5.20. as the backend using PHP 5.3.10(Hypertext Preprocessor) as interface. The results are displayed in such a manner that the proteins that were expressed in the irrigated and unirrigated leaves are compared and their results are displayed. It also includes calculated properties of proteins such as molecular weight, pI value, expression sequences obtained using MALDI-TOF and accession id. Then homology search is implemented to determine the most identical protein using protein database uniprot.The link which is provided for the accession id will connect directly to NCBI.The spot information includes homologous protein name, sequences coverage, mascot score(%),mascot result, sequence coverage. Theoretical molecular weight and pI.

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TNAU PDB- Tamil Nadu Agricultural University proteome database- Horse gram proteome

  1. 1.  TNAU PDB –Horse Gram ProteomeVisit TNAU Genomics at www.tnaugenomics.comVisit TNAU Genomics at www.tnaugenomics.com
  2. 2. IntroductionVisit TNAU Genomics at www.tnaugenomics.comVisit TNAU Genomics at www.tnaugenomics.com• TNAU PBD –Horse gram Proteome, openaccessible database.• Horse gram –drought tolerant crop.• Stress tolerance.• No organized database at present.• Information incorporated in user friendlyapproach.
  3. 3. PurposeVisit TNAU Genomics at www.tnaugenomics.com
  4. 4. MethodologiesVisit TNAU Genomics at www.tnaugenomics.com
  5. 5. Schema andUser’s AccessTnau genomicsTNAU PBDSpot informationHorse gramleaf2D gelMw pI AccessionVisit TNAU Genomics at www.tnaugenomics.comNCBIMYSQLPHPHTML
  6. 6. HomepageVisit TNAU Genomics at www.tnaugenomics.com
  7. 7. Gel ImageVisit TNAU Genomics at www.tnaugenomics.comVisit TNAU Genomics at www.tnaugenomics.comGel information
  8. 8. Spot informationVisit TNAU Genomics at www.tnaugenomics.com
  9. 9. Feature ProspectusVisit TNAU Genomics at www.tnaugenomics.com
  10. 10. Reference• Murukarthick, J., N. Senthil, M. Raveendran, P. Prabhakaran, G.Sreedevi, M. Sumanth Kumar, V. G. Shobhana, S. Dhanya, R. Khushboo,S. Arumugasamy, R. Ravikesavan and S. V. K. Jagadish. 2011. BiogenBase – An Interactive Maize Database for Phenomics Platform.International Journal of Computer Application. 2: 56–61Visit TNAU Genomics at www.tnaugenomics.com
  11. 11. Visit TNAU Genomics at www.tnaugenomics.comWon Best Poster Presentation Award on the topic “TNAU PDB” in theINTERNATIONAL CONFERENCE ON ADVANCES INBIOTECHNOLOGY AND PATENTING held in the Department ofBiotechnology and Genetic Engineering , Bharathidasan University ,Tiruchirappalli..Won Best Poster Presentation Award on the topic “TNAU PDB” in theINTERNATIONAL CONFERENCE ON ADVANCES INBIOTECHNOLOGY AND PATENTING held in the Department ofBiotechnology and Genetic Engineering , Bharathidasan University ,Tiruchirappalli..Under the guidanceDr.N.Senthil,Professor BiotechnologyCPMB S.JeyarekhaBTI-09-010

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