Floristic, genomic andfunctional turnover across   elevation gradients                  Marlien van der Merwe             ...
Scope• Conservation efforts that aim to improve  climate adaptation outcomes need to  consider evolutionary drivers that m...
Aims1. Species diversity and turn-over:  –   a plot based approach1. Neutral genetic diversity and turn-over:  –   a multi...
Approach• Finer scale: investigate climatic gradients  across one soil type representing similar  floristic assemblages• B...
BATS (Sydney sandstone)      Highly diverse floristically on similar substrate, and        strong gradients within a relat...
BATS Climatic Bins (Bioclim)    and sampled plots
BATS: plot sampling                                                      1600•   50 x 50 m plots                          ...
BATS: plot sampling•   50 x 50 m plots•   Current: 24; aim: 28•   Herbarium voucher and DNA    voucher for every woody    ...
Differentiation along the gradient:               Telopea speciosissima   The NSW WaratahRossetto et al. (2011) BMC Evol B...
BATS: multi-species population study• Investigate relative magnitude of gradient-  level variations in genetic diversity• ...
BATS: multi-species population study• Within-species turn-over for  species occurring across the  gradient                ...
Adaptive capacity of coastal vs. upland Telopea populations                   Rymer et al (in prep)
BATS: genetic adaptation along the gradient        4.6°C                            10.8°C                        8.6°C   ...
BATS: obtaining transcriptome data• Four libraries from each species : Petrophile pulchella, Isopogon anemonifolius and ad...
BATS: obtaining transcriptome data                                                     RNA                                ...
BATS: adaptation or plasticity?• Identify allelic and  expression level variation  between populations• Differentiate betw...
BATN: rainforest gradient• 24 (50x20) permanent plots on basalt• 278 species; 205 genera; 81 families• Full abundance (inc...
BATN: rainforest gradient
BATN: functional diversity                                      RainfallPeter D Wilson (MQU)
Bringing it all together• Integrating results:  – Associations between molecular,    environmental and functional diversit...
Acknowledgements• Foundation and Friends of RBG&DT; ARC  Linkage (Lowe, Rossetto, Summerell)• Volunteer team: Alexander Do...
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Maurizio Rossetto_Floristic and functional turnover across elevation gradients

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  • And how do they all relate to each other?
  • Maurizio Rossetto_Floristic and functional turnover across elevation gradients

    1. 1. Floristic, genomic andfunctional turnover across elevation gradients Marlien van der Merwe Hannah McPherson Maurizio Rossetto NATIONAL HERBARIUM OF NSW Andrew Lowe UNIVERSITY OF ADELAIDEAustralian Transect Network
    2. 2. Scope• Conservation efforts that aim to improve climate adaptation outcomes need to consider evolutionary drivers that maximise adaptive potential, as well as floristic and genetic variation• Use a multi-species, landscape-level approach to measure floristic and genetic turn-over along an environmental gradient
    3. 3. Aims1. Species diversity and turn-over: – a plot based approach1. Neutral genetic diversity and turn-over: – a multi-species landscape level approach1. Transcriptomic variation: – variation in expressed genes between climatic extremes1. Functional diversity and turn-over: – a multi-trait approach
    4. 4. Approach• Finer scale: investigate climatic gradients across one soil type representing similar floristic assemblages• Biodiversity and Adaptation Transect Sydney (BATS)• Biodiversity and Adaptation Transect Northern NSW (BATN)• Plus a range of satellite projects
    5. 5. BATS (Sydney sandstone) Highly diverse floristically on similar substrate, and strong gradients within a relatively small areaMean temperature in coldest quarter Annual precipitation
    6. 6. BATS Climatic Bins (Bioclim) and sampled plots
    7. 7. BATS: plot sampling 1600• 50 x 50 m plots 1200 DF P WH Rainfall• Annual rainfall Current: 24; aim: 28 LV MM CR GH C TN PR GW NJ BM MT PF KC MW BF WR• Herbarium voucher and DNA 800 AP AM GS BI GD voucher for every woody 400 species• Measure of abundance 0 0 5 10 15 20 25 plot• Temperature loggers at each plot 1200 NJ GW TN BF AP• 400+ species across all plots 800 MW• Species per plot: 40-70 Altitude altitude GD GS CR MT BI GH• Genera per plot: 28-50 400 BM PF AM PR• Families per plot:15-27 WH KC DF P LV WR MM C 0 0 5 10 15 20 25 plot number
    8. 8. BATS: plot sampling• 50 x 50 m plots• Current: 24; aim: 28• Herbarium voucher and DNA voucher for every woody species• Measure of abundance• Temperature loggers at each plot• 400+ species across all plots• Species per plot: 40-70• Genera per plot: 28-50• Families per plot:15-27
    9. 9. Differentiation along the gradient: Telopea speciosissima The NSW WaratahRossetto et al. (2011) BMC Evol Biol 11:126McIntosh et al. (2013) Ann Bot
    10. 10. BATS: multi-species population study• Investigate relative magnitude of gradient- level variations in genetic diversity• Investigate landscape-level associations between climatic gradients and genetic diversity, richness and functional diversity Hannah McPherson
    11. 11. BATS: multi-species population study• Within-species turn-over for species occurring across the gradient Isopogon anemonifolius• Sample genetic diversity for species distributed across the gradient and place into overall distributional context Petrophile pulchella• Use genomic (NGS reduction- based) methods to assess distribution of diversity, and current vs. historical dynamics• Inform transcriptome studies on same species Leptospermum trinervium
    12. 12. Adaptive capacity of coastal vs. upland Telopea populations Rymer et al (in prep)
    13. 13. BATS: genetic adaptation along the gradient 4.6°C 10.8°C 8.6°C 11°C Mean temperature in coldest quarterM vanderMerwe
    14. 14. BATS: obtaining transcriptome data• Four libraries from each species : Petrophile pulchella, Isopogon anemonifolius and additional Petrophile canescens (Proteaceae)• Each library: 25 seedlings from 5 mother plants• Future: major increase in species sampled (IBM support)
    15. 15. BATS: obtaining transcriptome data RNA extraction at two cotyledon stage Seedling germination at 20°C 12h day/12h night Pool extractions from a population One lane Illumina HiSeqBetween 20 million and 30million reads per library Contigs, coverage, Fst values, Blasts, etc …
    16. 16. BATS: adaptation or plasticity?• Identify allelic and expression level variation between populations• Differentiate between putative selection and neutral variation• Develop markers and screen across gradient• Identify multispecies patterns of ‘genetic response’ across gradient
    17. 17. BATN: rainforest gradient• 24 (50x20) permanent plots on basalt• 278 species; 205 genera; 81 families• Full abundance (including demographic, disturbance and temporal data)• Currently no DNA samples but pilot genomic study• Extensive functional dataset R Kooyman Reproduced from Crisp et al. 2004
    18. 18. BATN: rainforest gradient
    19. 19. BATN: functional diversity RainfallPeter D Wilson (MQU)
    20. 20. Bringing it all together• Integrating results: – Associations between molecular, environmental and functional diversities – Biodiversity, adaptive and landscape genetics – BATS / BATN / TREND (SA)
    21. 21. Acknowledgements• Foundation and Friends of RBG&DT; ARC Linkage (Lowe, Rossetto, Summerell)• Volunteer team: Alexander Dohms, Brendan Molloy, Lawrence Mou, Emma Oldman, Juelian Siow, Aurelia Webster-Hawes• Field work assistance: Louisa Murray, Michael Elgey, Susan Rutherford, Andrew Orme, Seanna McCune, Doug Benson, Melita Milner, Richard Milner• Doug Benson; Bob Coveny; Robert Kooyman

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