This document discusses phylogenetic tree construction for sequences A, B, and C using distance-based methods. It provides the sequences for A, B, and C, represents their evolutionary relationships using Newick format as (((A, B), C), D), and outlines the MEGA 11 protocol for converting alignment files, selecting tree construction methods, configuring bootstrapping, and computing the tree. The document was produced for a bioinformatics lab at Asian University for Women.

1. Lab#8: Phylogenetic Tree Construction
Asian University For Women
BINF 2000: Introduction to Bioinformatics
(Lab - Fall 2022)
Syed Mohammad Lokman
Instructor
Asian University for Women

4. The evolutionary relationships between A-D can also
be represented using Newick format as follows:
(((A, B), C), D)

5. Ref: https://doi.org/10.21203/rs.3.rs-105281/v1

6. Distance-Based Methods
A: AACTGGCTTA
B: AACTCGGTTA
C: CATTGGCATA

7. Distance-Based Methods
A: AACTGGCTTA; B: AACTCGGTTA; C: CATTGGCATA

8. Distance-Based Methods
A: AACTGGCTTA; B: AACTCGGTTA; C: CATTGGCATA

9. Distance-Based Methods
A: AACTGGCTTA; B: AACTCGGTTA; C: CATTGGCATA

10. Distance-Based Methods
A: AACTGGCTTA; B: AACTCGGTTA; C: CATTGGCATA

14. MEGA 11 Protocol:
1. Convert Alignment File to MEGA file
2. Select Tree Construction Method
3. Configure BootStrap
4. Compute to Construct the Tree

15. Thank You