2. 1.DISCOVERY AND ISSUES
ZIKV first isolated from Rhesus monkeys in
Uganda in 1947 which causes ZIKA fever.
Propagated to Pacific in 2007 and 2013.
Then to the Americas and Africa in 2015.
Now more than 13 countries in the America
have reported sporadic Zika virus infections
indicating rapid geographic expansion of Zika
virus.
3. 2.TAXANOMY AND NOMENCLATURE
Family :Flaviviridae
Order :Unassingned
Genus :Flavivirus
Species :Zika virus
It is so called “ZIKA” where it is found.
Zika virus bears a striking resemblance to
chikungunya.
Same clade of ZIKA are Spondweni virus ,St. Louis
encephalitis virus, Ilheus virus and Rocio virus.
4. 3.STRUCTURE AND COMPOSITION
It is an enveloped virus with icosahedral structure.
Contains non segmented Single-stranded, positive
sense RNA .
Complete genome sequence of ZIKA has been
published.
The open reading frame of the Zika virus reads as
follows:
5′-C-prM-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5-
3′.
6. 4.TRANSMISSION
Transmitted by Aedes species mosquitoes.
Major contribution by A. aegypti.
This is the same mosquito that transmits dengue,
chikungunya and yellow fever.
This usually bite during the morning and late
afternoon/evening hours.
Incubation period is about three to 12 days after the
bite of an infected mosquito.
8. 5.MECHANISM OF INFECTION
This ZIKA has enzootic mosquito-monkey-mosquito
cycle, with only occasional transmission to humans.
Dermal fibroblasts, epidermal keratinocytes are
permissible to ZIKV.
The virion attaches to host cell membrane receptors
via envelope protein E which induces virion
endocytosis.
The virus membrane fuses with the endosomal
membrane .
9. 5.MECHANISM OF INFECTION-CONTD…,
The ssRNA genome of the virus is released into the
cytoplasm.
It is then translated into a polyprotein. Translation
takes place by viral initiation.
Replication follows the positive stranded RNA virus
replication model and takes place as a cytoplasmic
viral factory in the endoplasmic reticulum resulting in
a dsRNA genome.
10. 5.MECHANISM OF INFECTION-CONTD…,
The dsRNA genome is then transcribed to ssRNA
New virions are transported to the Golgi apparatus
and then excreted by budding into the intracellular
space where the new virions cause infection.
ZIKV activates an antiviral innate immune
response with type 1 interferon in infected cells.
12. 5.HUMAN IMMUNE RESPONSE
Flavivirus cause acute sporadic disease and are
not persistent.
The outcome of flavivirus infection in an animal is
determined by a balance between the speed of viral
replication and spread, and the immune system
response.
Flaviviruses have evolved specific tactics to evade
the innate and adaptive immune response.
13. 5.HUMAN IMMUNE RESPONSE-CONTD..,
Flaviviruses, have genomic size constraints, and are
unable to acquire exogenous genes.
Smaller viruses evolve multifunctional genes that
regulate viral life cycle, yet also modulate the host
response.
The infected cells migrate to draining lymph nodes
where arise early immune response.
Then reach secondary lymphoid tissues, leading to
entry into the circulation via the efferent lymphatic
system and thoracic duct.
14. 5.HUMAN IMMUNE RESPONSE-CONTD..,
Now spread to visceral organs , then to neurotropic
flaviviruses disseminate to the central nervous system
The mechanism by which flaviviruses avoid being
targeted by its entry into brain and spinal cord remains
unclear
There is an assumption that the virus enters via a
haematogenous route.
15. 5.HUMAN IMMUNE RESPONSE-CONTD..,
Flaviviruses may cross the blood–brain barrier by
passive transport across the endothelium, by active
replication in endothelial cells.
'Trojan horse' mechanism in which the virus is
carried into the brain by infected inflammatory cells
The IFN-dependent innate immune response is
essential for protection against flavivirus infections.
Type I and II IFN inhibit flavivirus infection in cell
culture and in animals.
16. 5.HUMAN IMMUNE RESPONSE-CONTD..,
Type I IFN block flavivirus infection by
preventing translation and replication of
infectious viral RNA.
Type II IFN inhibit flavivirus replication via the
generation of proinflammatory and antiviral
molecules including nitric oxide .
17. 6.SYMPTOMS AND DISEASE
Fever.
Skin rashes.
Conjunctivitis.
Muscle and joint pain.
Malaise or headache.
These symptoms normally last for 2-7 days.
Affects nervous system eg:microcephaly in foetus.
Fig : 6.1 microcephaly head IMR
image
21. 7.DIAGNOSIS
Can be confirmed by presence Zika virus RNA in
the blood or other body fluids, such as urine or saliva
Zika virus RNA has been detected in urine up to 10
days after onset of the disease.
From day five post onset of disease, serological
investigations can be conducted by detection of
Zika-specific IgM antibodies and confirmation by
neutralisation, seroconversion or four-fold antibody
titer increase of Zika specific antibodies in paired
serum samples.
22. 8.AVAILABLE TREATMENT
There is no vaccine or specific prophylactic
treatment.
Bharat biotech international company of India
declared that they patented for medicine for ZIKV.
The treatment is symptomatic and mainly based
on pain relief, fever reduction and anti-histamines
for pruritic rash.
23. 9.PREVENTION
The best form of prevention is protection against
mosquito bites.
Repellents should contain DEET (N, N-diethyl-3-
methylbenzamide), IR3535 (3-[N-acetyl-N-butyl]-
aminopropionic acid ethyl ester) or icaridin (1-
piperidinecarboxylic acid, 2-(2-hydroxyethyl)-1-
methylpropylester).
24. 10.EXPERMENTAL TREATMENT
The Bharath biotech produced one inactivated and
one recombinant vaccines which are seeking for
patent.
People sick with Zika virus get plenty of rest, drink
enough fluids, and treat pain and fever with common
medicines.
Hypochlorite is a natural product for human
organism, and elaborated by immune cells for
protection against infection.
25. REFERENCES
1.R. M. Kofler, J. H. Aberle, S. W. Aberle, S. L. Allison, F. X. Heinz, and C. W. Mandl,
Proc. Natl. Acad. Sci. USA 7:1951-1956, 2004
2.Evasion of innate and adaptive immunity by flaviviruses,Michael
S Diamond1,1Departments of Medicine, Molecular Microbiology and Pathology &
Immunology, Washington University School of Medicine, Saint Louis, Missouri, USA
3. Oliveira Melo, A. S.; Malinger, G.; Ximenes, R.; Szejnfeld, P. O.; Alves Sampaio,
S.; Bispo de Filippis, A. M. (2016-01-01). "Zika virus intrauterine infection causes
fetal brain abnormality and microcephaly: tip of the iceberg?". Ultrasound in
Obstetrics & Gynecology 47 (1): 6–7. doi:10.1002/uog.15831. ISSN 1469-
0705. PMID 26731034.
4. Tick-borne flaviviruses: dissecting host immune responses and virus
countermeasures.
Robertson SJ1, Mitzel DN, Taylor RT, Best SM, Bloom ME
26. REFERENCES
5. Immune evasion strategies of flaviviruses.
Ye J1, Zhu B, Fu ZF, Chen H, Cao S.
6. Evasion of innate and adaptive immunity by
S Diamond1,Departments of Medicine, Molecular
Microbiology and Pathology & Immunology, Washington
University School of Medicine, Saint Louis, Missouri, USA