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PPT 1

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PPT 1

  1. 1. A STUDY ON THE BIOCONTROL ACTIVITYAND PLANT GROWTH PROMOTING ACTIVITY OF PSEUDOMONAS FLUORECENS ISOLATED FROM SOIL PRESENTED BY: DIEP VAN MINH Guided by Dr. T. VINOTH KUMAR, M.Sc., M.Phil. PGDCA, Ph.D. ASSOCIATE PROFESSOR AND DIRECTOR BIOTECHNOLOGY DIVISION SCHOOL OF BIOLOGICAL SCIENCES CMS COLLEGE OF SCIENCE AND COMMERCE COIMBATORE
  2. 2. INTRODUCTION  Biological control is defined broadly as the "use of natural or modified organisms, genes, or gene products” to reduce the effects of pests and diseases.  As we know bofore Pseudomonas Fluorecensen showed a high degree of antagonism against the phytopathogen  It promote plant growth.  Fungi of the genus Fusarium are necrotrophic pathogens that infect crops in all regions of cultivation to cause root rot, foot rot.  Fusarium species produces one or more mycotoxins. The Fusarium causes systemic infections of wheat plants by attacking their roots, stems, and spikes.
  3. 3. AIM OF THE PRESENT STUDY  Collection of soil sample  Isolation and characterization of the organism  Screening and identification of the organism  Screening and identification of the phytopathogen  Antagonistic activity of the organism against phytopathogen  Plant growth promoting effect of the organism
  4. 4. MATERIAL AND METHOD Isolation of Pseudomonas Fluorescence: the bacteria were isolated from soil sample which is collected from different areas parts of Kerala and Tamil Nadu by using King’B medium. Maintenance of pure culture: The selected colonies were steaked on to Centrimide Agar Medium plates to obtain pure culture. Screening and Identification of Pseudomonas fluorescens. 1. Gram staining 2.Culture Method 3. Biochemical characterization • IMVC test. • Oxidase test. • Catalase test. • Triple sugar iron test.
  5. 5. Screening and identification of pathogenic fungi.  The sample was collected from Tamil Nadu Agriculture University (TNAU).  Microscopic examination of pathogenic fungi.  Sub culturing of pathogen in medium.
  6. 6. Antagonistic activity of Pseudomonas against Fusarium mysporum o Antagonist, activity of Pseudomonas strain was tested against Fusarium mysporum by using dual culture technique. It can be done in 3ways. • Method I. • Method II. • Method III. Plant growth promoting activity.
  7. 7. Results Gram staining of the organism Cetrimide agar plates showing green colour colonies under UV
  8. 8. Pseudomonas Fluorecense under UV (360nm)
  9. 9. Bio-chemical characterization Citrate utilization test Triple sugar iron test
  10. 10. Indole test Methyl red test Voges-Proskauer Test Catalase test
  11. 11. Biochemical Test of Bacterial isolation Sl.No Test Results 1. Indole - 2. Methyl red - 3. Voges-proskauer - 4. Simmon citrate + 5. Catalase test + 6. Oxidase test + 7. TSI test +
  12. 12. Screening and identification of pathogenic fungi. PDA plate of Fusarium oxysporum
  13. 13. Antagonistic activity of Pseudomonas against Fusarium mysporum Method I Method II
  14. 14. Method III
  15. 15. Plant growth promoting activity
  16. 16. Plant Infection
  17. 17. Healthy Plant
  18. 18. SUMMARY AND CONCLUSION The present study the antagonistic activity of Pseudomonas fluorescens against the major phytopathogen Fusarium was tested. The Pseudomonas fluorescens was isolated from soil sample collected from different part of Kerala and Coimbatore.The phytopathogen was collected from TNAU, Coimbatore, dual plate culture technique revealed that the Pseudomonas Spp isolated show antagonistic activity against the phytopathogen. The plant growth promoting efficacy of the Pseudomonas Spp was also studied . The organism showed enhance the growth of Soya bean when compare to the seed that was untreated.

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