Prof. dr. Simona CavaluFaculty of Medicine and PharmacyUniversity of OradeaROMANIA
As the average age of population grows, the need for medical devices to replace damaged or worn tissues increases.
As patients have become more and more demanding regarding esthetic and biocompatibility aspects of their dental restorations .
Alumina/zirconiaceramics were successfully used in total hip/knee arthroplastyin the last decades.
For dental application: root canal posts, orthodontic brackets, implant abutments and all-ceramic restorations.
Bioceramicinteraction with living tissue
Surface modifications and post –synthesis treatments for better performances
Composition: 80%Al2O3–20%YSZ with 5%TiO2addition
Spark plasma sintering method at 1350-1400◦C.
Structural characterization by FTIR and XRD spectroscopy
Morphological details of the surface investigated by SEM
Fracture toughness 5.3 MPam½ (under a load of 19.6 N)
Vickers hardness 16.7 GPa(under a load of 9.8N).
O. Ormanci, S. Cavalu-Mater SciEng C 40 (2014)
1200 1000 800 600 400
Modifications of stretching
vibration modes AlO6 octaedra
80Al2O3-20YSZ with 5% TiO2
Texture of protein (fibrinogen) coating on alumina/zirconiaceramics- electrodeposition
FTIR spectroscopy and deconvolution
αhelix % βsheet% βturns % Random % Side chain% 19.99.2
Surface treatment with SnF2and NaBF4-ATR FTIR evidence
Fig. 1 ATR FTIR spectra of SnF2 and NaBF4 powders as received from the supplier .
Fig. 2 ATR FTIR spectra recorded on specimen surface before and after treatment using SnF2 and NaBF4.
Surface treatment- XPS evidence
1200 1000 800 600 400 200 0
Sn 4d Zr 4p F 2s
Binding Energy (eV)
In vitro test: cells culture
Human fibroblast (HLF) seeded in a concentration of 2x104/cm2 cells on the surface of each sample (SnF2 respectively NaBF4 treated ) and cultured for 3h, 7h and 24h.
Cell nuclei were stained with 5 mMDraq5 diluted 1:1000 in distilled water for 5 min at room temperature.
Visual inspection demonstrating initial adherence and proliferation of fibroblasts.
Fibroblasts adherence/proliferation evidence by confocalmicroscopySnF2NaBF4
The label * indicates p<0.001 versus control, **indicates p<0.01 and *** indicates a p<0.001 with respect to specimen 1. SnF2NaBF4
In vivo tests: animal model (rabbit)
Implant 1-SnF2 treatment
Implant 2-NaBF4 treatment
New bone proliferation
New bone proliferation
siteHistology; implant 1 = SnF2 treatmentimplant 2 = NaBF4 treatment
Ca/P= 1.62- 1.80
Bone morphology after 4 and
8 weeks post -surgery
XRD spectrum of the femoral bone
0 20 40 60 80 100
Animal model-The presence of young, compact lamellar bone and osteocytesnear the implant surface indicated good biocompatibility, and certainly the presence of the implant did not disturb the processes of bone formation at the interface, for both organic/inorganic treatment.
Microstructure details (including Haversiancanals) of bone and bone marrow tissue and elemental composition at the interface indicated Ca/P =1.62 -1.77 SummaryConclusions:Organic(proteic)filmorfluorideassurfaceconditioningmightbeanalternativeapproachtoinducethebioactivityandimprovethebiocompatibilityofdensebioceramicsdesignedtoloadbearingbonereplacement(hipjoint,dentalabutments)andtooptimizethebiologicalresponseforspecificapplicationsofbiomedicalimplants.
O. Ormanci, I. Akin, F. Sahin, O. Yucel, V. Simon, Simona Cavalu, G. Goller, Spark Plasma sintered A2O3-YSZ-TiO2 composites: Processing, characterization and in vivo evaluation, Materials Science and Engineering C, 40 (2014) 16-23.
Simona Cavalu, C. Ratiu, O. Ponta, V. Simon, D. Rugina, V. Miclaus, I. Akin, G. Goller, Improving osseointegrationof alumina/zirconiaceramic implants by fluoride surface treatment, Digest Journal of Nanomaterialsand BiostructuresVol. 9, No. 2 (2014) 797 –808.
Simona Cavalu, V. Simon, F. Banica, I. Akin, G. Goller, Surface modification of alumina/zirconiabioceramicsupon different fluoride-based treatments, Int. J. Appl. Ceram. Technol., 11  402–411 (2014).
Simona Cavalu, V. Simon, I. Akin, G. Goller, Adherence properties of acrylic bone cement to alumina ceramics designed for clinical application, ActaPhysicaPolonicaA, nr.2,vol.125 (2014) 603-605
S. Cavalu, V. Simon, C. Ratiu, I. Oswald, R. Gabor, O. Ponta, I. Akin, G. Goller, Correlation between structural properties and in vivo biocompatibility of alumina/zirconiabioceramics, Key Engineering Materials vols. 493-494, 1-6(2012)
Acknowledgments: UEFISCDIproject PNII-ID-PCE 2011-3-0441 contract nr. 237/2011 and Bilateral Cooperation RO-TR.
•Prof. dr. VioricaSimonBabes-BolyaiUniversity, Faculty of Physics & Institute of Interdisciplinary Research in Bio-Nano- Sciences, Cluj-Napoca, Romania.
•Dr. Cristian Ratiu, Ioan Oswaldand Silviu Vlad, University of Oradea, Faculty of Medicine and Pharmaceutics, Oradea, Romania.
•Dr. Dumitrita Rugina, USAMV Cluj- Napoca.
•Prof. dr. GultekinGollerand assist. prof. Ipek Akin, Istanbul Technical University, Materials Science Department.