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Le cell

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Le cell

  1. 1. LE CELL DEMONSTRATION
  2. 2. The lupus erythermatosus (LE) cell: Is a mature neutrophilic polymorphonuclear leukocyte, which has phagocytized a spherical, homogeneous-appearing inclusion, itself derived from nuclear material of degenerating leukocytes and coated with antinuclear antibody; a characterisitic of lupus erythematosus
  3. 3. LE cell seen in….. • Systemic lupus erythematosis • Rheumatoid arthritis • Discoid lupus erythematosis
  4. 4. Methods of demonstration of LE cell • Clotted blood • Defibrinated blood • Citrated or heparinized blood • Rotary method
  5. 5. Clotted blood • Simplest method • 10 ml of clotted blood is placed in water bath at 32o C for 1 to 2hrs. • Mash the clot with fine wire • The cells expressed from the clot are placed in a Wintrobe haematocrit tube and centrifuged at high speed (2,000 r.p.m.) for 5 minutes. • The buffy coat is then ,carefully removed with a fine glass pipette and smeared on glass slides. • Any of the stains for routine blood films are satisfactory.
  6. 6. Defibrinated blood • The blood is collected in a flexible container (conical flask) of appropriate size into which is placed a sufficient quantity of glass beads. • Blood is added to it. • It is rotated in 8 shape for 15min. • Incubated for 2hrs at 370 • Smears are made after centrifugation same as clotted blood • Stain with wright stain.
  7. 7. Rotary method • 5 glass beads 3mm in diameter are added to a heparinised sample of blood. • Tube is then roated at 50rpm for 30 min at 37o • Buffy coat smears are prepared.
  8. 8. • LE cell are seen at the edge of a smear. • 500 polymorhs are counted before a negative result is given. • Rosette – group of polymorhs will collect around altered nuclear material.

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