1. NONONO TOKEY

2. Ok sir

3. FISHPRESERVATION

4. GRAPH NHI HAI YHA
 Keep on it

5. FISHPRESERVATION
⚫FirstdocumentedbyBoyle(1663).
⚫Firstconceptofspermpreservationby
Lazzarospallanzini(1760).
⚫ Most significantdiscoverywastheuseof
glycerolascryoprotectant byPolgeand
smith(1949)

6. PRINCIPLE
1. noFREEZI
NG
2. No
STORING
3. THAWING

7. CONT
..
⚫ Theabilityofglycerol toprotectcellsfromfreezing
injurywasdiscoveredaccidentally
.
⚫ Thesubsequentdevelopmentof
FISHPRESERVATION techniqueshashad ahuge
impactinmanyfields, mostnotablyin
reproductivemedicine.
⚫ OK Lets do it
⚫

8. TUSSI mat BATAO