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CANDIDIASIS – ClinicalManifestations andlab diagnosis of Oral Candidiasis
CONTENTS TO STUDY• Clinical manifestations of candida• Lab diagnosis of oral candidiasis
Clinical Manifestations of CandidaCandidiasisInfectiousdiseasesMucocutaneousmanifestationsCutaneousmanifestationsSystemicm...
Mucocutaneous ManifestationsMucocutaneousmanifestationsOral candidiasisAngular cheilitisOesophagitisVulvovaginitis,Balanit...
Cutaneous ManifestationsCutaneousManifestationsIntertrigoParonychia andOnychomycosisDiaper dermatitisCandidialgranuloma
Systemic ManifestationsSystemicmanifestationsUrinary tract candidiasisCandiduriaEndocarditisPulmonary candidiasisMeningiti...
Allergic DiseasesAllergicdiseasesCandididsEczemaAsthmaGastritis
Lab diagnosis of Oral CandidiasisSamples• Whitish patches from mucous membrane of mouth• SputumMethod of collection• Steri...
Lab diagnosis continued…Fungal culture• Sabouraud Dextrose Agar + Antibiotics ( Cream, Pasty, Smooth colonies in 3-4days o...
Lab diagnosis continued…Biochemical tests• Glucose and Maltose fermented with acid and gasproduction, sucrose and lactose ...
Lab diagnosis continued…Detection of metabolites• Detection of D-mannose and D-arabinitol in sera by gasliquid chromatogra...
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Candidiasis – clinical manifestations and lab diagnosis of oral candidiasis

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Candidiasis – clinical manifestations and lab diagnosis of oral candidiasis

  1. 1. CANDIDIASIS – ClinicalManifestations andlab diagnosis of Oral Candidiasis
  2. 2. CONTENTS TO STUDY• Clinical manifestations of candida• Lab diagnosis of oral candidiasis
  3. 3. Clinical Manifestations of CandidaCandidiasisInfectiousdiseasesMucocutaneousmanifestationsCutaneousmanifestationsSystemicmanifestationsAllergicdiseases
  4. 4. Mucocutaneous ManifestationsMucocutaneousmanifestationsOral candidiasisAngular cheilitisOesophagitisVulvovaginitis,Balanitis,BalanoposthitisChronicmucocutaneouscandidiasis (CMC)Ocular candidiasis
  5. 5. Cutaneous ManifestationsCutaneousManifestationsIntertrigoParonychia andOnychomycosisDiaper dermatitisCandidialgranuloma
  6. 6. Systemic ManifestationsSystemicmanifestationsUrinary tract candidiasisCandiduriaEndocarditisPulmonary candidiasisMeningitisCandidemia and SepticemiaDisseminated candidiasisArthritisOsteomyelitisEndopthalmitisNosocomial candidiasis
  7. 7. Allergic DiseasesAllergicdiseasesCandididsEczemaAsthmaGastritis
  8. 8. Lab diagnosis of Oral CandidiasisSamples• Whitish patches from mucous membrane of mouth• SputumMethod of collection• Sterile swabsDirect examination (Microscopy)• KOH wet mount• Gram staining ( Presence of yeast cells and pseudohyphae)• Other stains: PAS and Gomori’s methenamine silver stain
  9. 9. Lab diagnosis continued…Fungal culture• Sabouraud Dextrose Agar + Antibiotics ( Cream, Pasty, Smooth colonies in 3-4days of incubation at 37 C)• Cornmeal Agar (Characteristic terminal chlamydospores, yeast cells andpseudohyphae in clusters at 25 C)• CHROMagar (light green to bluish green colonies)Germ tube test (Reynold’s-Braude Phenomenon)• Culture of candida treated with sheep/normal human serum is incubated at 37C for 2-4 hours• No constriction seen at the point of attachment to the yeast cell• Appear in 2 hours for C.albicansChlamydospore formation• Suspected strain of Candida isolates gron on CMA or rice starch agar andincubated at 25 C• Formation of large, highly refractile, thick walled, terminal chlamydospores in2-3 days of incubation
  10. 10. Lab diagnosis continued…Biochemical tests• Glucose and Maltose fermented with acid and gasproduction, sucrose and lactose not fermented.• Pale pink coloration in Tetrazolium reduction mediumTyping of Candida strains• Tests include serotyping and isoenzyme profiling• RFLP and Southern blotting technique usedImmunodiagnosis• PCR based tests for candida-DNA detection• Detection of Candida albican-derived molecules• ELISA, RIA, CIE, PHA and LPA
  11. 11. Lab diagnosis continued…Detection of metabolites• Detection of D-mannose and D-arabinitol in sera by gasliquid chromatography• G-test for detection of glucanSkin tests• Testing delayed hypersensitivity to candidial antigensAnimal Pathogenicity• Tests on rabbits and mice for susceptibility to differentcandidia species
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