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Power point presentation for science research

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Power point presentation for science research

  1. 1. Using Polycaprolactone for Tissue Engineering<br />A Research Study by: Satish Bhat<br />UCONN - STORRS<br />
  2. 2. Abstract<br />Biomedical engineering and tissue regeneration are novel fields of research. Lives are lost everyday across the world because of the lack of donor organs. By producing these organs artificially, those people would be able to continue their lives. It was hypothesized that polycaprolactone would be a viable material to use in tissue engineering. This hypothesis was based on previous research that had been done. In this study, many specific analytical tests had to be done to find valid results. For example, a NMR (Nuclear Magnetic Resonance) test was done as well as thermal analysis. During the course of the study, several important results were gained. Thus far, polycaprolactone has been shown as a valid material to be used in tissue engineering. The results of the NMR test showed the presence of stable ions in the polymer as polylactic acid. Polylactic acid is a polymer that is currently being successfully used in tissue engineering. Also, thermal analysis tests showed that the polymer is a thermoplastic and has very similar characteristics to polylactic acid. Results obtained suggest that polycaprolactone is a strong contender for tissue scaffolding. NMR results show the magnetic pulse for polycaprolactone was radiated back out at the same frequency as polylactic acid. Polylactic acid is successfully being used, and because polycaprolactone has similar characteristics; it can possibly be used in scaffolding. Lives can be saved with this research, and polycaprolactone also is better for the environment because of its biodegradability characteristics.<br />
  3. 3. Motivation<br /><ul><li> Polycaprolactone is a seven-membered polymer with the formula (CH2)5CO2
  4. 4. Lives are lost because organs die due to old age and disease – donor organs save those people
  5. 5. Donors are not always available, synthetic organs come into play</li></ul>Example of Tissue Scaffold<br />
  6. 6. Hypothesis<br /><ul><li> It has been hypothesized that polycaprolactone will have similar characteristics to polylactic acid, and will be a viable material for scaffolding
  7. 7. Prior research has shown that PCL scaffolds “possess mechanical properties within the lower range of…bone, suggesting that they may have the ability to withstand early functional loading.”</li></ul>How to create grafts for human use<br />
  8. 8. Research Question<br /><ul><li> Can polycaprolactone be used in tissue engineering?
  9. 9. Being investigated by comparing to already used polymer, polylactic acid
  10. 10. IF similar characteristics, then strong contender for use in scaffolding.</li></ul>How to create polycaprolactone<br />
  11. 11. Materials<br /><ul><li> 0.0620g of Schwartz catalyst (Cp2ZrClH) at room temperature
  12. 12. 0.0161g of initiator (terepthaldehyde) at room temperature
  13. 13. 3mL of solvent (toluene) at room temperature
  14. 14. Argon gas to replace atmosphere
  15. 15. Access to a clock
  16. 16. Liquid nitrogen
  17. 17. 1.0300g of caprolactone at room temperature
  18. 18. NMR test
  19. 19. GPC test
  20. 20. DSC test
  21. 21. Thermal analysis
  22. 22. Mechanical analysis
  23. 23. Stress test
  24. 24. Lab notebook
  25. 25. Ring
  26. 26. Test tube
  27. 27. Hot plate that can spin test tubes
  28. 28. Thermometer
  29. 29. Two 5mL syringes
  30. 30. Analytical balance
  31. 31. DSC pans</li></ul>Chemical Structure of Caprolactone<br />
  32. 32. Procedure<br />
  33. 33. Calculations<br />This figure shows the calculations used to obtain the amount of each substance.<br />
  34. 34. NMR Results<br />This figure is the results of the NMR (Nuclear Magnetic Resonance) Test<br />
  35. 35. Structures of Materials<br />Polycaprolactone <br />Caprolactone/Zr = 1/2 <br />Cp2ZrHCl (Schwartz Reagent)<br />Caprolactone <br />
  36. 36. GPC Results<br />
  37. 37. Mechanical Testing Results<br />PCL Mn = 218,000<br />modulus<br />
  38. 38. DSC Results<br />Heating Scan<br />Cooling Scan<br />PCL<br />PCL<br />P(DL)LA<br />Heat Flow<br />P(DL)LA<br />PCL-b-P(DL)LA<br />PCL-b-P(DL)LA<br />PCL-co-P(DL)LA<br />PCL-co-P(DL)LA<br />Temperature (C)<br />Temperature (C)<br />
  39. 39. Conclusions<br /><ul><li> NMR Results show that peaks (hydrogen bonds) are in the same location as polylactic acid
  40. 40. Peaks at 6.8, 4.0, 1.8, 0.5, 0.6, and 0.0 (same locations as polylactic acid)
  41. 41. Possibly strong contender for tissue scaffolding</li></ul>Polycaprolactone<br />
  42. 42. Error Analysis<br /><ul><li> Further research includes finding more materials that can be used in tissue engineering
  43. 43. Argon gas was not injected perfectly due to the ring on the test tube not being tight enough
  44. 44. All measurements are never perfect</li></ul>Front light mount from PCL<br />
  45. 45. Acknowledgements<br />Research was assisted by Assistant Professor at UCONN – Storrs Dr. Alexandru Asandei and Graduate Student Christopher Simpson. <br />Research was conducted at the Institute of Material Sciences – Polymer Building, 97 North Eagleville Road, Storrs, CT.<br />
  46. 46. Citations<br />[1] = "Tissue Engineering | Science/AAAS." Science. Web. 13 Jan. 2011. <http://www.sciencemag.org/content/260/5110/920>.<br />[2] = "Access : Bridging the Gap : Nature." Nature Publishing Group : Science Journals, Jobs, and Information. Web. 13 Jan. 2011. <http://www.nature.com/nature/journal/v433/n7021/full/433019a.html>.<br />[3] = "Book Article." Wiley Online Library. Web. 16 Jan. 2011. <http://onlinelibrary.wiley.com/doi/10.1002/14356007.a05_031/full>.<br />[4] = (177), In Scopus. "ScienceDirect - International Journal of Pharmaceutics : Poly-ε-caprolactone Microspheres and Nanospheres: an Overview." ScienceDirect - Home. Web. 16 Jan. 2011. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7W-4C82NG4-1&_user=10&_coverDate=06/18/2004&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=9ebb0ef4eebbbf9125e4194ad60da65d&searchtype=a>.<br />[5] = (5), In Scopus. "ScienceDirect - Process Biochemistry : Biodegradability and Mechanical Properties of Polycaprolactone Composites Encapsulating Phosphate-solubilizing Bacterium Bacillus Sp. PG01." ScienceDirect - Home. Web. 16 Jan. 2011. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THB-4MVF4YJ-1&_user=10&_coverDate=04/30/2007&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1608852147&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=73559a6c1ffbf79377bbad975fa8cba9&searchtype=a>.<br />[6]= (157), In Scopus. "ScienceDirect - Biomaterials : A Novel Degradable Polycaprolactone Networks for Tissue Engineering." ScienceDirect - Home. Web. 16 Jan. 2011. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TWB-47DH8T7-C&_user=10&_coverDate=02/28/2003&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1608854140&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7e54ef58465c59b352217964b017717c&searchtype=a>.<br />[7] = William, Jessica M., Adebisi Adewunmi, Rachel M. Schek, Colleen L. Flanagan, and Stephen E. Feinberg. "Bone Tissue Engineering Using Polycaprolactone Scaffolds Fabricated via Selective Laser Sintering." ScienceDirect - Home. MIT - Webs, 8 Nov. 2004. Web. 16 Jan. 2011. <http://web.mit.edu/course/3/3.042/team1_06/reference/modulus/Bone%20tissue%20engineering%20using%20polycaprolactone%20scaffolds%20fabricated%20via%20selective%20laser%20sintering.pdf>.<br />[8] = Kweon H. "Http://www.ncbi.nlm.nih.gov/pubmed/12485798." Pub Med. 24 Feb. 2003. Web. 16 Jan. 2011. http://www.ncbi.nlm.nih.gov/pubmed/12485798<br />[9]= Domingos, Marco. "Polycaprolactone Scaffolds Fabricated via Bioextrusion for Tissue Engineering Applications." Hindawi Publishing Corporation. 11 Oct. 2009. Web. 16 Jan. 2011. <http://www.hindawi.com/journals/ijbm/2009/239643.html>.<br />[10]= "Fabrication of Modified and Functionalized Polycaprolactone Nanofibre Scaffolds for Vascular Tissue Engineering." IOPscience::.. Welcome! Web. 16 Jan. 2011. <http://iopscience.iop.org/0957-4484/16/10/028>.<br />

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