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Meditory Journal (Vol. 1, No. 2, Desember 2013)

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Meditory Journal (Vol. 1, No. 2, Desember 2013)

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Meditory Journal (Vol. 1, No. 2, Desember 2013)

  1. 1. The Journal of Medical Laboratory Meditory Editorial Pembaca yang terhormat, Salam hangat dari kami kepada para pembaca meditory journal, tidak terasa sudah 6 bulan yang lalu dari terbitan meditory journal yang perdana. Meditory journal hadir kembali pada edisi Vol. 1 No. 2, yang pada kesempatan ini terdapat sembilan informasi kesehatan yang berhubungan dengan laboratorium baik dalam bentuk artikel ilmiah maupun hasil penelitian di tengah-tengah para pembaca, dengan harapan dapat menjadi salah satu sumber bacaan dalam menambah pengetahuan/wawasan dan pengembangan ilmu dibidang kesehatan, khususnya berkaitan dengan laboratorium. Pada pada penerbitan edisi kali ini, sembilan artikel ilmiah yang dimuat, disajikan beberapa bidang kajian, antara lain: pada bidang kimia klinik, Ni Luh Putu Erayanti, dkk., membahas tentang gambaran proteinuria pada ibu hamil trimester III di puskesmas I Denpasar Selatan dan Yully Trisna Dewi, dkk., membahas tentang hubungan indeks massa tubuh (IMT) dengan kadar glukosa darah sewaktu pada lansia Pada bidang hematologi, Putu Purwanthi, dkk., membahas tentang hubungan kadar hemoglobin dan perilaku sarapan terhadap prestasi belajar siswa di SMPN 2 Sukawati. Pembahasan tentang swamedikasi hiperkolesterolemia dengan ekstrak air daun gonda dan buah juwet pada Rattus norvegicus, disajikan oleh: Lely Cintari, dkk., dan artikel optimasi macam pengikat (PVP K-30-Amylum manihot) dan macam pengisi (Laktosa: Manitol-Laktosa: Sorbitol) dalam formula tablet hisap ekstrak daun sirih merah (Piper crocatum), disajikan oleh: Gusti Ayu Made Ratih Kusuma Ratna Dewi. Pada bidang mikroorganisme (virologi dan bakteriologi), Luh Ade Wilan Krisna, dkk., membahas tentang isolasi virus Avian Influenza subtipe H5 pada unggas di wilayah Surabaya periode Tahun 2011- 2012; Ni Made Dwijayanti, dkk., membahas tentang perbedaan berbagai konsentrasi ekstrak bawang putih (Allium sativum linn) terhadap zona hambat pertumbuhan Escherichia coli; dan Ayu Sukma Wedha Kristyanti, dkk., membahas tentang kejadian infeksi saluran kemih pada ibu hamil di desa Dalung, Kecamatan kuta Utara, Kabupaten Badung-Bali. Pada bidang kimia makanan dan minuman, pembahasan tentang tinjauan kandungan boraks pada bakso yang dijual di Desa Sidakarya Kota Denpasar, disajikan oleh: Ni Luh Putu Ari Sundari, dkk. Harapan kami, semoga para pembaca dapat mengambil manfaat dari penerbitan meditory journal ini, namun kami sadar bahwa masih banyak kekurangan yang kami alami. Kami juga tiada hentinya meminta doa, dukungan, serta saran dan kritik yang membangun dari berbagai pihak, yaitu: pembaca, penulis maupun pengelola meditory journal, agar kami selalu dapat memberikan yang terbaik dan lebih baik kedepannya. Akhir kata, kami sampaikan ucapan selamat membaca dan terima kasih. Denpasar, Desember 2013 Redaksi Alamat Redaksi: Jurusan Analis Kesehatan Poltekkes Denpasar Jl. Sanitasi No. 1 Sidakarya, Denpasar-Bali 80224 Indonesia Telp. +62-361-710 527, Fax. +62-361- 710 448, E-mail:meditoryjournal@gmail.com
  2. 2. Meditory | Vol. 1, No. 2, Desember 2013 IV Gambaran Proteinuria Pada Ibu Hamil Trimester III di Puskesmas I Denpasar Selatan Ni Luh Putu Erayanti., Ida Ayu Made Sri Arjani., IGA. Dewi Sarihati. Hubungan Indeks Massaa Tubuh (IMT) Dengan Kadar Glukosa Darah Sewaktu Pada Lansia Yully Trisna Dewi., IGA. Dewi Sarihati., Burhannuddin. Hubungan Kadar Hemoglobin dan Perilaku Sarapan Terhadap Prestasi Belajar Siswa di SMPN 2 Sukawati Putu Purwanthi., Cok. Dewi Widhya Hana Sundari., IGA. Sri Dhyanaputri. Swamedikasi Hiperkolesterolemia Dengan Ekstrak Air Daun Gonda dan Buah Juwet Pada Rattus norvegicus Lely Cintari., A.A. Nanak A., I.A. Eka P. Optimasi Macam Pengikat (PVP K-30-Amylum manihot) dan Macam Pengisi (Laktosa: Manitol-Laktosa: Sorbitol) Dalam Formula Tablet Hisap Ekstrak Daun Sirih Merah (Piper crocatum) Gusti Ayu Made Ratih Kusuma Ratna Dewi. Isolasi Virus Avian Influenza Subtipe H5 Pada Unggas di Wilayah Surabaya Periode Tahun 2011-2012 Luh Ade Wilan Krisna., dkk. Perbedaan Berbagai Konsentrasi Ekstrak Bawang Putih (Allium sativum linn) Terhadap Zona Hambat Pertumbuhan Escherichia coli Ni Made Dwijayanti., I Wayan Merta., Burhannudin. Kejadian Infeksi Saluran Kemih Pada Ibu Hamil di Desa Dalung, Kecamatan kuta Utara, Kabupaten Badung-Bali Ayu Sukma Wedha Kristyanti., I Nyoman Jirna., Heri Setiyo Bekti. Tinjauan Kandungan Boraks Pada Bakso Yang Dijual di Desa Sidakarya Kota Denpasar Ni Luh Putu Ari Sundari., IGA. Sri Dhyanaputri., A.A. Made Sucipta. Diterbitkan oleh: Jurusan Analis Kesehatan Poltekkes Denpasar Persatuan Ahli Teknologi Laboratorium Kesehatan Indonesia (PATELKI) DPW Bali Original Article Daftar Isi Vol. 1, No. 2, Desember 2013 Meditory The Journal of Medical Laboratory halaman 70 - 76 77 - 83 84 - 93 94 - 107 108 - 117 118 - 125 126 - 135 136 - 145 146 - 154 11. 12. 13. 14. 15. 16. 17. 18. 19.
  3. 3. The Journal of Medical Laboratory Meditory Meditory | Vol. 1, No. 2, Desember 2013 V Pedoman Bagi Penulis Ruang lingkup Meditory (the Journal of Medical Laboratory) adalah jurnal ilmiah resmi yang diterbitkan oleh Jurusan Analis Kesehatan Poltekkes Denpasar bekerjasama dengan Persatuan Ahli Laboratorium Kesehatan (PATELKI) DPW Bali. Jurnal ini diterbitkan untuk menyebarluaskan informasi mengenai penemuan/penelitian dibidang teknologi laboratorium yang meliputi ilmu pengetahuan patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi. Naskah (teks) yang dimuat dalam Meditory adalah laporan yang belum pernah dipublikasikan sebelumnya baik sebagian maupun keseluruhan. Bentuk naskah Naskah disusun menggunakan bahasa Indonesia, diketik spasi 1,5 dengan garis tepi minimum 2,5 cm. Panjang naskah tidak melebihi 10 halaman yang dicetak pada kertas A4 (21x30 cm). kirimkan 2 (dua) kopi naskah beserta soft file (micrososft word) atau melaui e-mail. Naskah dikirimkan kepada: • dr. IGA. Dewi Sarihati, M.Biomed Ketua Dewan Redaksi Meditory, Jl. Sanitasi No. 1 Sidakarya, Denpasar-Bali Telp. (0361) 710 527, Fax. (0361) 710 448 atau E-mail: meditoryjournal@gmail.com Sistematika 1. Naskah hasil penelitian memuat: a. Judul b. Abstrak c. Pendahuluan d. Metode e. Hasil dan pembahasan f. Simpulan dan saran g. Daftar pustaka 2. Naskah non hasil penelitian memuat: a. Judul b. Abstrak c. pendahuluan d. Pembahasan e. Simpulan dan saran f. Daftar pustaka Judul dan Nama Pengarang Judul ditulis lengkap dan jelas, tanpa singkatan. Nama pengarang (atau pengarang-pengarang) ditulis lengkap disertai gelar akademiknya, institusi tempat pengarang bekerja, dan alamat pengarang serta no telepon, fax, atau e-mail untuk memudahkan korespodensi. Abstrak Naskah tinjauan pustaka dan artikel asli hendaknya disertai abstark, ditulis pada halaman pertama di bawah nama dan institusi. Panjang abstark 200-250 kata. Abstark ditulis secara terstruktur, dengan urutan sebagai berikut: latar belakang, tujuan, metode, hasil, kesimpulan dan kata kunci.
  4. 4. Meditory | Vol. 1, No. 2, Desember 2013 VI Tabel dan gambar Tabel harus singkat dan jelas, hendaknya ditulis di atas dan catatan di bawahnya. Jelaskan semua singkat yang dipergunakan. Gambar hendaknya jelas, hendaknya judul gambar di tulis di bawahnya. Asal rujukan atau gambar dituliskan di bawahnya table atau gambar hendaknya dibuat dengan program power point, photoshop atau CS (menggunakan format jpeg, tiff denagn resolusi minimal 300dpi) Daftar pustaka Rujukan di dalam nas (teks) harus disusun menurut angka sesuai dengan urutan penampilannya dalam nas, dan ditulis menurut system Vancouver. Tuliskan semua nama pengarang bila kurang dari tujuh; bila tujuh atau lebih, tuliskan hanya 3 pengarang pertama dan tambahkan dkk. Tuliskan judul artikel dan halaman awal- akhir. Akurasi data kepustakaan menjadi tanggung jawab pengarang. Sumber rujukan sedapat mungkin merupakan pustaka-pustaka terbitan 10 tahun terakhir. Rujukan yang diutamakan adalah sumber-sumber primer berupa laporan penelitian (termasuk skripsi, tesis, disertasi) atau artikel-artikel penelitian dan/ atau majalah ilmiah. • Buku dan Monograf 1. Bannister, B.A., Begg, N.T.Y., Gillespie, S.H. Infectius Disease. Edisi pertama. Oxford: Blackwell science; 1996. 2. Departement of Health. Caring for People with Severe mental illness. London: Departement of Helath; 1994. 3. Murti B. Prinsip dan Metode Riset Epidemologi. Jilid pertama, Edisi kedua. Yogyakarta: Gajah Mada University Press; 2003. 4. Singarimbun, M., dan Efendi, S. Metode Penelitian Survai. Edisi revisi. Jakarta: LP3ES; 1989. 5. White, R., dan Efan, C. Conflict Management. London: Chapman and Hall; 1996. 6. Sudiono, H., dkk. Patologi Klinik Urinalisis, Jakarta: Bagian Patologi Klinik Fakultas Ukrida; 2006. 7. Merta, I Wayan. Pengaruh Berbagai Kontainer dan Sumber Air Terhadap Perkembang biakan Larva Aedes aegepty [thesis]. Fakultas Pascasarjana Universitas Mahasaraswati; 2010. • Majalah/jurnal/koran 1. Connors, N.M. Risk Perciptaion, Risk Taking and Risk Management, Soc-Sci Med 1998, 34 (6): 595-607. 2. Sucipta Made, A.A. Risk Factors For Cefotaxime Resistance in Children With Pneumonia, Paediatrica Indonesiana 2012, 52 (5): 255-259. 3. Sjaaf, A.C. Analisis Layanan Biaya Kesehatan Rumah Sakit, Medika 1991: 123-164. 4. Baraldi, E., and Filippone, M. Chronic Lung Disease after Premature Birth. N Engl J Med 2007; 357:1946-55. • Publikasi Lain 1. Morse S.S. Factors in the emergence of infectious disease. Emerg Infect Dis [serial online] Jan-Mar 1995 [cited 5 Jan 1996]; 1910:[24 screnn]. Didapat dari: URL: http: //www.cdc.gov/ncidod/EID/ eid.htm. 2. Hemodynamis III: the ups and downs
  5. 5. Meditory | Vol. 1, No. 2, Desember 2013 VII Jadwal Penerbitan : Jurnal terbit 2 kali setahun, tiap bulan Juni dan bulan Desembar of hemodynamics [program komputer]. Versi 2.2. Orlando (FL); Computerized Educational System; 1993. 3. Amsjari, F. [lemlite@unair.edu]. berbagai Macam Uji Stastitik Untuk Uji Hipotesis. E-mail pribadi untuk Choirul Hadi [choirul@yahoo.com] [cited 28 Nop 2004].
  6. 6. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 70 Number 1 Gambaran Proteinuria Pada Ibu Hamil Trimester III di Puskesmas I Denpasar Selatan Ni Luh Putu Erayanti, Ida Ayu Made Sri Arjani, IGA. Dewi Sarihati Abstract Background proteinuria in pregnant women is a state of protein in the urine identifying the symptoms of preeclampsia. Some causes of the proteinuria are glomerular diseases, tubular disease, non-renal disease (fever, strenuous exercise and heart failure), and urinary tract diseases. Objective this study aims at describing the proteinuria in the pregnant women of the third trimester in the South Denpasar Public Health Center 1. Methods this research applied a descriptive survey by means of cross sectional method. The amount of samples taken was using non-probability sampling method with saturated sampling technique where the data were collected from 30 respondents during the period of February to May 2013. The samples were examined semi-quantitatively by using boiled/heating test with 6 percent acetic acid. Results of the examination to 30 samples indicated that 21 urine samples (70%) was negative, five urine samples (16,66%) positive + (1+), two urine samples (6.67%) positive ++ (2+) and two urine samples (6.67%) positive ++++ (4+). Conclusions to pregnant women that her proteinuria results are mainly positive (4+), we suggest in order to consult a midwife or obstetrician to get a better treatment. Besides that, avoiding stress and have an enough rest can prevent the occurrence of preeclampsia. Keywords: proteinuria; pregnant women; third trimester.
  7. 7. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 77 Number 2 HUBUNGAN INDEKS MASSA TUBUH (IMT) DENGAN KADAR GLUKOSA DARAH SEWAKTU PADA LANSIA Yully Trisna Dewi, IGA. Dewi Sarihati, Burhannuddin Abstract Background recently, obesity has become an epidemic with the assumed increasing prevalence of obesity shall reach 50% by 2025 within developed countries population. Obesity is one of the causes of insulin resistance that occurs in the elderly which implies the increasing risk of diseases, such as hyperglycemia and Diabetes Mellitus. Objective this study aims to examine the relationship of Body Mass Index (BMI) towards blood glucose level of the elderly in Banjar Buana Kubu, Tegal Harum Village, District of West Denpasar. Methods is an analytical survey research with Cross Sectional Design. The research was conducted in the Banjar Buana Kubu, Tegal Harum Village District of West Denpasar. The population in this study shall be all elderly in Banjar Buana Kubu as many as 50 people. Sample are 34 elderly and non probability sampling method with saturation sampling technique based on inclusion and exclusion criteria to obtain. Result showed that 17, 4% elderly with overweight have high blood glucose level, and 11,1% elderly with normal BMI. Coclusion of data showed that p < 0.05, (r = 0.497, p = 0.003) there is a significant relationship between BMI and blood glucose level. Keywords: Body Mass Index; Blood glucose level; Elderly.
  8. 8. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 84 Number 3 HUBUNGAN KADAR HEMOGLOBIN DAN PERILAKU SARAPAN TERHADAP PRESTASI BELAJAR SISWA DI SMPN 2 SUKAWATI Putu Purwanthi.,Cok. Dewi Widhya Hana Sundari., IGA. Sri Dhyanaputri Abstract Background learning activities need an adequate nutrition from breakfast habits. In addition to losing concentration, nutrient deficiencies can also cause anemia of nutrients that affect to learning achievement of student. Anemia can be determine based on the decrease in hemoglobin values below 12,00 g/dL. Objective this study aims to know the relationship between hemoglobin value and breakfast habits with learning achievement of students in SMPN 2 Sukawati. Methods this research applied a with cross Sectional design that conducted in February 2013 to 38 respondents based on simple random sampling technique. Results the Chi square test result showed that there is a relationship between hemoglobin value with learning achievement of students (sig 0,007), there is a relationship between breakfast habits with learning achievement of students (sig 0,006) and there was not relationship between the breakfast habits with hemoglobin value (sig 0,938). Conclusions the researches recomended to the following reasearch, should be investigate about more factors that can be affect to hemoglobin value and learning achievement of students, which is carried out in a longer period of time. Keywords: hemoglobin; breakfast; learning achievement.
  9. 9. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 94 Number 4 SWAMEDIKASI HIPERKOLESTEROLEMIA DENGAN EKSTRAK AIR DAUN GONDA DAN BUAH JUWET SECARA PRAKLINIS Lely Cintari., A.A.Nanak A., I.A.Eka P. Abstract Background determination of Effect of extract chlorophyll irrigate the leaf gonda (Splenocle a Zeylanica Gaertn) and antocyanin of fruit juwet (Syzygium Cumini ( L.) Skeels (Myrtaceae) or more knowledgeable as black plum fruit by comparing between extract irrigate the leaf of gonda of fruit juwet ( EADGBJ) and sediaan patent " E" what have circulated in marketing. Obstetrical of chlorophylin in leaf gonda representing compound of chlorophyl show the ability antioxidatif by in vitro and ex vivo, and also energy hipocholesterolemic by in is vivo 1 . content of Fruit of juwet antocyanin representing compound of acid of amino phytosterols, saponins, steroids, and triterpenoids. Result of research show by having a meaning of that there is correlation by having a meaning of between extract concentration given by prosentase pursue the happening of free radical and peroksidasi lipid. Others, Alfa-Phytosterol that is of a kind sterol having the character of anticholesterolemic, able to prevent the cholesterol excess 2 . Objective to identify the influence of extract chlorophyll irrigate the leaf of gonda and antocyanin of juwet fruit ( EABJDG) to reduce cholesterol level Rattus Norvegicus Hypercholesterolemia. Methode this research represent the true eksperimental study with the device of Randomized Control Trial ( RCT). Research executed at Laboratory of Nutrition in Health Polytechnic Denpasar with the minimum amount sampel 30 tail divided into 5 same group, then fasted by during 24 hour fixed is given to drink the white water sufficiently. Group I (standard), group II (cholesterol + patent " E"), group III (cholesterol+EABJ), group IV (cholesterol+EADG) and group V (cholesterol + EADGBJ). As comparator used drug " E" what have circulated in Indonesia. Blood taken twice that is before and after treatment gift that is on to 0 and 28 days. The total Cholesterol serum level specified by kit from CHOD-PAP Merckotest with the test of fotometric enzimatic ( enzymatic photometric test). Result the study is indicate that degradation of cholesterol also improvement after gift of extract of Juwet fruit, extract of gonda leaf and mixture of juwet fruit extract and leaf of gonda extract by significantly (p< 0.05). Juwet fruit extract and gonda leaf extract can degrade the profile lipid after treatment during 28 days. Keywords : Swamedication hypercholesterolemia; Ekstract etanol of leaf gonda; fruit of juwet extrac; Praclinical.
  10. 10. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 108 Number 5 OPTIMASI MACAM PENGIKAT (PVP K-30 - AMYLUM MANIHOT) DAN MACAM PENGISI (LAKTOSA:MANITOL - LAKTOSA:SORBITOL) DALAM FORMULA TABLET HISAP EKSTRAK DAUN SIRIH MERAH (PIPER CROCATUM) Gusti Ayu Made Ratih Kusuma Ratna Dewi Abstract Background red betle has long been used by people as a medicine to cure various kinds of diseases. Objective the aim of this study was to know the influence of binder type (PVP K-30 - amylum manihot), filler type (lactose:mannitol - lactose:sorbitol) and their interactions in the lozenges formula of red betle (Piper crocatum) leaf extract and obtain the optimum composition of the lozenges formula that has appropriate physical properties. Methods optimization techniques used this research is factorial design method with two factors and two levels are binder type with PVP K-30 5% as a low level and amylum manihot 5% as a high level, and filler type with lactose:mannitol (2:1) as a low level and lactose:sorbitol (2:1) as a high level. The observed response to determine the optimum formula is the hardness, friability, and disintegration time. Result showed that PVP K-30 and amylum manihot as a tablet binder, combination of lactose:mannitol and lactose:sorbitol as a tablet filler material, as well as their interactions significantly influenced the hardness, friability, and disintegration time of red betle (Piper crocatum) leaf extract lozenges. Conclusion based on the Design – Expert optimization program obtained the lozenges formula with optimum physical properties that is by using a combination of PVP K-30 - lactose:sorbitol, a combination of PVP K-30 - lactose:mannitol, and combinations of amylum manihot – lactose:sorbitol. Keywords: amylum manihot; factorial design; lactose; lozenges; mannitol; optimization; Piper crocatum; PVP K-30; red betle leaf; sorbitol.
  11. 11. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 118 Number 6 ISOLASI VIRUS AVIAN INFLUENZA SUBTIPE H5 PADA UNGGAS dI WILAYAH SURABAYA PERIODE TAHUN 2011-2012 Luh Ade Wilan Krisna., Masaoki Yamaoka., Aldise Mareta Nastri., Emmanuel Djoko Poetranto., Laksmi Wulandari., Resti Yudhawati., Landia Setiawati., Retno Asih Setyoningrum. Abstract Background the H5N1 subtype of avian influenza viruses have recently become an important zoonotic pathogen. Since 2003, H5N1 highly pathogenic avian influenza (HPAI) has been endemic in poultry found in Southeast Asia (Soda, 2008). Among the Southeast Asian countries, Indonesia is the most affected by H5N1 HPAI, with the virus being found in 31 of its 33 provinces. 1 Yet, little is known about the diversity of H5N1 HPAI viruses circulating in backyard farms, where chickens and ducks often intermingle. Objective here, we report on H5N1 HPAI viral strains isolated from domestic poultry (backyard chickens and ducks) in west Surabaya, East Java, Indonesia during 2011-2012. Methods we obtained a total of 30 cloacal swabs. These samples were inoculated into 11-day-old embryonated chicken eggs, incubated at 37°C for two days, and followed by harvesting of allantoic fluid. Eleven samples were positive for hemagglutinin (HA). We obtained two positive samples for the H5 subtype by RT-PCR using a H5-specific primer pair. HA and neuraminidase (NA) genes from the samples were subsequently sequenced and processed for phylogenetic analysis. Results molecular characterization of the H5N1 viruses confirmed that they harbored the cleavage site in HA genes characteristic of HPAI, although none had mutations that accelerate mammalian adaptation. Phylogenetic analyses revealed that H5N1 viruses belong to clade 2.3.1, which is prevalent in Indonesia. Gene homology analysis revealed a more than 90% match with the sequences of human isolates. Conclusions our findings suggest that virus surveillance studies are useful for understanding the genetic characteristics and evolution of endemic influenza viruses in Indonesia. Keyswords: Avian Influenza; poultry; H5N1.
  12. 12. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 126 Number 7 PERBEDAAN BERBGAGAI KONSENTRASI EKSTRAK BAWANG PUTIH (Allium Sativum Linn) TERHADAP ZONE HAMBAT PERTUMBUHAN Escherichia coli Ni Made Dwijayanti., I Waya Merta.,Burhannudin Abstract Background Allium sativum linn is a well know advantageous herbal . Previous studies show that Allium sativum linn had antibacterial effect to Escherichia coli . Objective the purpose of this research was to know the differentiation of multy concentrate garlic extract (Allium sativum linn) against zone inhibition germination to Escherichia coli. Methods this research was an experimental study with posttest only control group design with difusi disk method . The sample of this experimental study was extract of Allium sativum linn with multy concentrate. The antibacterial activity test uses difusi disk method , these were divided into five test group with concentration 20% , 40% , 60% , 80% , 100% , positive control , and negative control . Minimum Inhibition Results consentration was determined by ability to prevent Escherichia coli from growing on Mueller Hinton Agar .Statistic analysis was done by using One Way Anova test and LSD (Least Significant Different). Conclusions concentration of effective or sensitive is concentration 20% significantly inhibited bacterial growth and had a sensitive compared to positive control . All concentration did show different ability in bactericidal activity . Minimum Inhibition Concentration to Escherichia coli is concentration 20% . Keywords: concentrate; garlic; Escherichia coli.
  13. 13. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 136 Number 8 KEJADIAN INFEKSI SALURAN KEMIH PADA IBU HAMIL DI DESA DALUNG, KECAMATAN KUTA UTARA, KABUPATEN BADUNG – BALI Ayu Sukma Wedha Kristyanti., I Nyoman Jirna., Heri Setiyo Bekti. Abstract Background urinaryTract Infection (UTI) that caused by bacteria is commonly happened in women, such as pregnant women. Untreated UTI can leads to premature birth and low weight in newborn baby. Objective this study aims to determine whether there is any indication of the incidence of UTI in the pregnancy women. Methods research is descriptif study with cross sectional design. Total samples urine 38 from pregnant women at various weeks of gestation who live in the Dalung Village, Sub District of North Kuta, Badung- Bali are cultured. Samples urine that examined are morning urine samples and clean mid-stream urine. Bacterial examination of urine samples is done using colony count method. Results of the study showed that 5 pregnant women (13%) have colony count results more than 100.000 CFU/ml. The colony count results more than 100.000 CFU/ml presences of bacteria in the urine (bacteriuria). Untreated cases of bacteriuria can leads to infections in the urinary tract. Conclusion to pregnancy women to do some laboratory tests if there are some symptoms of UTI and for pregnancy women to be more concerned with the hygiene of urinary tract. Keywords: Colony Count Method; Pregnant Women; Urinary Tract Infection; Urine.
  14. 14. Meditory Original Article The Journal of Medical Laboratory Meditory | Vol. 1, No. 2, Desember 2013 146 Number 9 TINJAUAN KANDUNGAN BORAKS PADA BAKSO YANG DIJUAL DI DESA SIDAKARYA KOTA DENPASAR Ni Luh Putu Ari Sundari., I Gusti Ayu Sri Dhyanaputri., A.A.Made Sucipta. Abstract Backgroud meatballs are one of the typical food in Indonesia. The use of Food Additives in food production need to be monitored. Based on Permenkes 1168/Menkes/Per/X/1999 about food additive, boric acid and its compounds is one of the prohibited use of additives in food, because borax can give harmful effects for human health. Meatballs are food products made of the main ingredients of meat crushed, mixed with other additional materials, round-shaped circle and then boiled. Objectives the purpose of this research was to prove the existence of borax content on the meatballs that sold in the Sidakarya village. Methods this study was an observational study. The sample studied was meatballs non packaging sold in the stalls meatballs and branded packaging meatballs sold in traditional markets. Total sample meatballs used in this study are 15 samples. then testing with the laboratory flame test method and the results were analyzed descriptively. Results the results showed there were two positive meatballs samples (13.3%), while 13 other sample obtained negative results boraks contain (86.7%) Conculsions borax was found in two meatballs sample of branded packaging meatballs sold in traditional markets in the Sidakarya village, the branded packaging meatballs was without registration number of the national authorities. Keywords: borax, meatballs, flame test method

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