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20150924 smb noviocell juliette van den dolder


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Presentation Science Meets Business @ Health Valley From Molecule to Business 20150924

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20150924 smb noviocell juliette van den dolder

  1. 1. 1 Polymeric nanofiber matrix as the 3D solution for stem cell research Juliette van den Dolder, PhD
  2. 2. 2 Noviocell BV • Spin-off: founded Sept 2015 • Prof A. Rowan, Dept Molecular materials, RU and Prof W. Feitz and Dr E. Oosterwijk, Dept Urology, RIMLS, Radboud UMC • Our mission is to accelerate life science research in (stem) cell growth and differentiation by making 3D cell culture feasible for every (stem) cell biology researcher. • Polyisocyanopeptide hydrogel is a fully synthetic biomimetic extracellular matrix with identical biomechanical properties as natural matrices
  3. 3. 3 Stem cell researchers need 3D scaffold to mimic human nature • Stem cells for tissue engineering and regenerative medicine 3D human organs2D cell culture ware
  4. 4. 4 Extracellular Matrix - mimetic material Schematic representation of extracellular matrix. Nature Nanotech 2011, 6:13 Polyisocyanopeptide (PIC) hydrogel • Physical support for the cells • Chemical composition of the ECM
  5. 5. 5 Batch variation, animal origin, cell recovery are drawbacks of current 3D scaffolds Batch to batch variation Animal origin Cell recovery with harsh methods such as scraping, dissolving in solvents or enzyme treatment
  6. 6. 6 PIC hydrogel is reproducible, synthetic & allows ease of cell recovery  Fully reversible thermo-sensitive which allows for ease of cell or tissue recovery  Easy downstream processing after culturing (DNA, protein analysis or microscopy) hotcold Polyisocyanopeptide (PIC) hydrogels  No batch to batch variation  Synthetic origin "Responsive biomimetic networks from polyisocyanopeptide hydrogels", Nature 2013, 493:651-655 P. Kouwer Dept. Molecular Materials, Radboud University.
  7. 7. 7 PIC hydrogel functions as a cell instructive and porous scaffold Tubulur structures of Huvec cells in PIC hydrogel with RGD peptide, VEGF and FGF Can be easily functionalized for different cell types 3b "Novel Polyisocyanopeptide Hydrogels for 3D cell culture", W.Sun et al. Dept of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2015, submitted.
  8. 8. 8 Cells grow, spread and organize in PIC hydrogel PIC hydrogel tested with several cells types
  9. 9. 9 PIC hydrogel performs uniquely like human collagen PIC hydrogel follows same strain stiffness as human collagen “Ultra-responsive soft matter from strain-stiffening hydrogels” M. Jaspers Nature Communications 2014, 5:5808 collagen
  10. 10. 10 Current status and future challenge Batch to batch variation Biomechanical properties Biophysical properties Biocompatibility Sterility Product stability PIC Hydrogel with fibrin PIC Hydrogel with RGD peptide Biofunctionalized PIC hydrogel for specific cell types Defining essential product requirementsDevelopment of products 2016 2017 2018 UPSCALING
  11. 11. 11 Questions? Noviocell BV Dr. Juliette van den Dolder Reinier Postlaan 2 6525 GC Nijmegen, the Netherlands T: +31-646155064 Questions? Thank you for your attention