1. HAN BioCentre
Centre of Expertise in Biotechnology & Analysis
Christien Lokman
28 May 2015

2. WHY?
 For well educated students
 For teachers who know the ‘state of
the art’
 For companies, to support in research
and innovation

3. Industry
and
universities
Research
HAN
BioCentre
Education:
Bachelor,
Master,
Courses
BIODISCOVERY

4. DISCOVERY

5. Development of smart screeningssystems
 antimicrobial/antifungal
– Medical
– Agro & Food
– other
 C. elegans screeningssystem
– Medical (obesity)
– Safety/Toxicity

6. Oil from yeast
 In cooperation with Wageningen UR
 Companies: Nuplex, van Wijhe, Latexfalt,
Holland Colors, Parenco, Dyadic, MicCell, MVO

7. ANALYSIS

8. Biological screening systems
Analytical chemistry
Bio-informatics
Molecular biology
 PCR/qPCR
 SDS-PAGE
 LC-MS
 HPLC
 FPLC
 GC
 Fluorescence microscopy
Amphotericin
0
20000
40000
60000
80000
100000
120000
0:00
0:37
1:15
1:53
2:30
3:08
3:46
4:23
5:01
5:39
6:17
6:54
7:32
8:10
8:47
9:25
10:03
10:40
11:18
11:56
12:33
13:11
13:49
14:26
15:04
15:42
time (h)
Luminescens
water
NaPb
DMSO 1:100
Amph 20 µM
Amph 10 µM
Amph 5 µM
Amph 2,5 µM
Amph 1 µM
Amph 0,5 µM
Amph 0,1 µM

9. PRODUCTION

10. Fermentation & downstream processing
• From 1 to 20 L (E. coli, lactic acid bacteria, Bacillus,
Helicobacter, filamentous fungi, basidiomycetes,
yeast)
• Downstream processing
– IEX
– GPC
– Microfiltration
– Ultrafiltration
• Strain selection
• Genetic modification of m.o.

11. APPLICATION

12. In collaboration with companies and
universities
 Food/Feed
 Agro
 Health
 Sustainable energy

13. • AM-Pharma
• Aquamar
• Argos Oil
• Avatar Biotechnologies (USA)
• Avebe
• Basidiofactory
• Benilabs
• B.O.B.
• Cellution Biotech
• C4C
• Christian Hansen (DK)
• CSK Food Enrichment
• CU+TOP
• Cyto-Barr
• DMV International
• DSM
• Dyadic Nederland
• European Veterinary Laboratory
• Falco Diagnostics
• Filtex
• Innovative SeedTechnology
• TNO Quality of Life
• Koppert
• LabTie
• Meddens Diagnostics
• MicCell
• Micreos
• Nsure
• Nuplex
• NVWA
• NYtor
• Parenco
• Productschap Tuinbouw
• ProteoNic
• Quaker
• Rainbow Sustainable solutions
• RIVM
• Shell
• Uniqema/Croda
• Unilever
• Van Wijhe
• Vitaleech Bioscience
• Vivalt screening
• Xenikos

14. Consortia
 Centre of Biobased Economy
 Green Health
 IJ5-lab
 others

15. What do we offer?
- Contract research
- Cooperative research
• Basidiofactory
• Vivalt screening
- Lab facilities
• Basidiofactory
• Vivalt screening
- Workshops
APPLICATIONDISCOVERY ANALYSIS PRODUCTION

16. What do companies bring?
- Knowledge/new developments
- Guest lectures
- Facility sharing
- Internships
APPLICATIONDISCOVERY ANALYSIS PRODUCTION

18. www.hanbiocentre.nl
28th of January 2016
Conference HAN BioCentre 10 years