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SPIO Uptake by Macrophages
Maamoun A.Qamar, MD
Morteza Naghavi, MD
The peak iron level in the serum after
administering the recommended dose of
Feridex to humans (i.e. 0.56 mg of Fe/ kg) is...
•To compare it to the ratio of monocytes in
1ml of blood, we would have to consider the
following:
 3-8 % of leukocytes a...
In order to simulate the in vivo SPIO-to-
Macrophage ratio, we applied the same ratio
to our ex vivo study.
We used 100 mi...
Protocol:
• We used 8 flasks of CBM macrophages
• After preparing the cells, Feridex was
added with the proper concentrati...
time concentration
20
Min
1
Hour
6
Hours
24
Hours
50µl 100µl 250µl 500µl control control
Macrophage Uptake of Feridex
After 20 Min
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20 min
Feridex (µL)
Macrophage Uptake of Feridex
After 60 Min
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60 min
Feridex (µL)
Macrophage Uptake of Feridex
After 6 Hours
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6 Hours
Feridex (µL)
Macrophage Uptake of Feridex
After 24 Hours
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24 Hours
Feridex (...
Macrophage uptake of Feridex
increases with increasing the
Amount of Feridex
0
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2...
Macrophage Uptake of Feridex
Increases With Time
0
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20 Min 60 Min 6 Hours 24 Hours
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50...
Spio uptake by macrophages
Spio uptake by macrophages
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Spio uptake by macrophages

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Spio uptake by macrophages

  1. 1. SPIO Uptake by Macrophages Maamoun A.Qamar, MD Morteza Naghavi, MD
  2. 2. The peak iron level in the serum after administering the recommended dose of Feridex to humans (i.e. 0.56 mg of Fe/ kg) is: 5.6 µg/ml
  3. 3. •To compare it to the ratio of monocytes in 1ml of blood, we would have to consider the following:  3-8 % of leukocytes are monocytes  There are 4000- 10000 leukocytes/ µl of blood That means we have 120- 800 monocytes/ µl or 12Χ 104 – 8 Χ 105 / ml After injection of Feridex, there will be 1Χ10-4 mmol Fe/ ml
  4. 4. In order to simulate the in vivo SPIO-to- Macrophage ratio, we applied the same ratio to our ex vivo study. We used 100 million macrophages in each one of our 24 tubes used in the study. We chose 4 concentrations of Feridex that provided us with a wide range of SPIO-to- Macrophage Ratio and included the in vivo ratio too.
  5. 5. Protocol: • We used 8 flasks of CBM macrophages • After preparing the cells, Feridex was added with the proper concentration to each labeled tube • Incubation was at 37° C • For each time point, we centrifuged the tubes at 1000 rpm’s for 5 min • Washed with 1X PBS for 5 min • Resuspended in 2% paraformaldehyde to fix the cells
  6. 6. time concentration 20 Min 1 Hour 6 Hours 24 Hours 50µl 100µl 250µl 500µl control control
  7. 7. Macrophage Uptake of Feridex After 20 Min 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 20 min Feridex (µL)
  8. 8. Macrophage Uptake of Feridex After 60 Min 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 60 min Feridex (µL)
  9. 9. Macrophage Uptake of Feridex After 6 Hours 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 6 Hours Feridex (µL)
  10. 10. Macrophage Uptake of Feridex After 24 Hours 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 24 Hours Feridex (µL)
  11. 11. Macrophage uptake of Feridex increases with increasing the Amount of Feridex 0 10 20 30 40 50 60 70 80 90 50 250 control 20 min 60 min 6 hours 24 hours
  12. 12. Macrophage Uptake of Feridex Increases With Time 0 10 20 30 40 50 60 70 80 90 20 Min 60 Min 6 Hours 24 Hours 50 100 250 500 control control

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