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Spio cytokinejan28

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SHAPE Society

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Spio cytokinejan28

  1. 1. Cytokine-SPIO Protocol •Purpose: To compare the iron particles present in macrophages of apo E-deficient atherosclerotic plaques under baseline conditions (control group) and after the administration of the cytokines TNF-alpha and IL-1beta (test group). •Protocol: Eight retired female breeders, approximately 12-month old were divided in 2 groups. Five received I.P. 0.2 µg each of mouse recombinant TNF- and IL-1ß; the 3 control received 0.5 mL saline containing 1% BSA. Three hours later, all the animals were I.V. injected with Feridex 1 mMol/kg of iron. Seven days later, the recipients were euthanized with CO2 and perfused under physiological pressure. The entire aorta up to the iliac bifurcation was formalin-fixed, serially sectioned transversally and stained for H&E and iron.
  2. 2. No cytokines C1Cx10
  3. 3. No cytokines C1Cx40
  4. 4. No cytokines C3Ex10
  5. 5. No cytokines Only plaque with Fe C3Ex40
  6. 6. No cytokines C3E
  7. 7. TNFα/IL-1ß T2Fx10
  8. 8. TNFα/IL-1ß T2Fx40
  9. 9. TNFα/IL-1ß T2Fx40ab
  10. 10. TNFα/IL-1ß T2Fx40cd
  11. 11. TNFα/IL-1ß T3Fx10
  12. 12. Coronary artery involvement T3F coronary
  13. 13. TNFα/IL-1ß T3Fx40
  14. 14. TNFα/IL-1ß T4Bx10
  15. 15. TNFα/IL-1ß T4Bx40
  16. 16. TNFα/IL-1ß T4B
  17. 17. TNFα/IL-1ß T4F
  18. 18. TNFα/IL-1ß T4Cx10
  19. 19. TNFα/IL-1ß T4Cx40
  20. 20. TNFα/IL-1ß T4Fx10
  21. 21. TNFα/IL-1ß T4Fx10bc
  22. 22. Infarct and coronary involvement T4F
  23. 23. TNFα/IL-1ß T4Fx40bcd
  24. 24. TNFα/IL-1ß T3Ex10
  25. 25. TNFα/IL-1ß T3Efex40
  26. 26. TNFα/IL-1ß T3Bx10
  27. 27. TNFα/IL-1ß T3Bx40ab
  28. 28. TNFα/IL-1ß T3Bfex40ab
  29. 29. TNFα/IL-1ß T4E

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