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Nir poster

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SHAPE Society

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Nir poster

  1. 1. A Novel Method for Detection of CRP and Other Elements of Arterial Inflammation Paul Cherukuri1 , Silvio Litovsky1 , MD, Ward Casscells1 , MD, James T. Willerson1 , MD, Morteza Naghavi1 , MD, Robert Lodder2 , PhD University of Texas – Houston1, Department of Cardiology and the Texas Heart Institute1 , Houston, TX University of Kentucky2 , College of Pharmacy2 , Lexington, KY Near-IR Spectrometry Near-IR spectrometry uses the absorption, emission, or scattering of light in the near-infrared portion of the electromagnetic spectrum (700 - 3000 nm) by atoms or molecules to determine sample composition or characteristics. Near-IR Spectrometry Near-IR spectrometry uses the absorption, emission, or scattering of light in the near-infrared portion of the electromagnetic spectrum (700 - 3000 nm) by atoms or molecules to determine sample composition or characteristics. Texas Heart Institute® C Optical Biopsy Results Predicted Actual Thick cap overlying lipid core Thick Fibrous Plaque Vulnerable Plaque(thin cap, overlying large lipid core Eachstripthenscannedunder referencevisiblemicroscopefor histologicalcorrelation EachaorticstripwascannedinaNIR spectrometer1100-2500nm ShowDrawingHERE of Plaquesegmentedintostrips Eachaortacutintocircumferentialsegments Humanaorta-invitro n=6aorta EachaorticstripwasscannedinaNIR spectrometer1100-2500nm Catheterusedwtihatunablelaserlightsource andspectracollectedasaortawaswithdrawn Insertcatheterintofemoralarteryuptothelevelof oftheaorticarch Rabbitaorta-invivo n=6aorta MethodlogyforPhotonicdeterminationofplaquecomponents Plaquesamples Material and Methods Results Conclusions • Introduction • The purpose of this study was to test the hypothesis that dynamic near-infrared (NIR) photonic analysis is capable of detecting inflammatory vulnerable blood components such as CRP and BNP. Furthermore, this study also tested the hypothesis that NIR was capable of accurately predicting vulnerable structural components such as cholesterol, collagen and elastin, and macrophage content within the arterial walls of both in vitro (human) and in vivo (rabbit) aorta samples. What is a vulnerable plaque? A vulnerable atherosclerotic plaque is a highly active region of inflammation within an arterial wall that in the presence of an appropriate triggering mechanism, ruptures, erodes, or ulcerates. Structure:Vulnerable plaques typically contain a substantial lipid core (>40%) composed primarily of cholesterol and cholesterol esters covered by a thin (typically <100 microns) fibrous cap, which is composed mainly of collagen and elastin with a dense macrophage infiltrate located subendothelially. Clinical significance: Rapid thrombus formation after rupture overlying the plaque produces a rapid occlusion and is the major cause of sudden cardiac death. What is vulnerable blood? Constituents within the blood that have been shown to be indicators of inflammation or elevated in cardiac disease, such as CRP(C-reactive protein) and BNP(B-naturietic peptide. Clinical significance: CRP is an indicator of inflammation. It has been shown that people who died sudden cardiac death had an abundance of CRP in the blood, although few had had outward signs of heart problems. The finding bolsters the case for using CRP as a diagnostic tool to detect heart ailments because autopsies revealed that high CRP concentrations in blood coincide with the presence of dangerously unstable atherosclerotic plaques in the coronary arteries. Near-IR Spectrometry Near-IR spectrometry uses the absorption, emission, or scattering of light in the near-infrared portion of the electromagnetic spectrum (700 - 3000 nm) by atoms or molecules to determine sample composition or characteristics.

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